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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000, MBL Life Science, cat. No. M214-3) or anti-GAPDH-HRP (1:50,000, Invitrogen, cat. No. MA5-15738-HRP). Membranes were visualized with 0.5x ECL (Amersham ...
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bioRxiv - Bioengineering 2020Quote: ... RT-qPCR was performed on a 1:3 dilution of the extracted RNA using TaqPath™ 1-Step RT-qPCR Master Mix (Thermo Fisher Scientific) with the following forward and reverse primer sequences ...
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bioRxiv - Cell Biology 2021Quote: ... 3 x 1 h) and incubated with the secondary antibody (Alexa-labelled goat anti-mouse 555 plus, A32727, Thermo Fisher Scientific, 1:1000), an anti-GFP antibody conjugated with Alexa 488 (A-21311 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nepa Gene) with 3 µg plasmid DNA and 1 × 106 cells in 100 µl Opti-MEM (1×) Reduced Serum Medium (Gibco® ThermoFisher Scientific). The electroporated cells were cultured in a non-selective complete medium in a 100 mm × 20 mm cell culture dish (Falcon ...
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bioRxiv - Cancer Biology 2020Quote: ... The assembled PDMS layers were treated with oxygen plasma (100 watt, 1 ccm, 140 torr, 10 seconds) for irreversible bonding to a glass slide (Fisherbrand 1×3”, Fisher Scientific, Pittsburgh, PA). Prior to flow experiments ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed three times for 3 minutes and then incubated 1 hour at RT with secondary antibody (1:500, #A21202, #A21449, #A10042, #A32731, Life Technologies, Carlsbad, California) and Hoechst 33342 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... The wells were washed with 1% BSA/LBB and incubated for one hour with L9393 (1:5000 dilution) in 3% BSA/LBB followed by incubation with Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG (Invitrogen, 1:5000 dilution) in 3% BSA/LBB for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Deposited ECM was fixed by exposure to 100% methanol at −20°C and stained with Alexa Fluor488-conjugated anti-fibronectin (EBioscience, clone FN-3, 1:500 dilution) or Alexa Fluor594-conjugated (Invitrogen, 1:1000 dilution) anti-ColI/III antibodies (EMD ...
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bioRxiv - Molecular Biology 2024Quote: ... Brain slices were blocked with 5% donkey serum in 0.3% PBS-T for 1 h at RT and then incubated with a sheep anti-GFRAL antibody (1:500, Thermofisher, Cat. PA5-47769) and/or a rabbit anti-c-Fos antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
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bioRxiv - Pathology 2019Quote: The infected rice sheaths were incubated with CellTracker™ Blue CMAC Dye (7-amino-4-chloromethylcoumarin, Molecular Probes, C2110) at a final working concentration of 10 μM for 2 h at 37 °C ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Biochemistry 2019Quote: ... 7 μl of Superscript III reverse transcription master mix (containing 4 μl of 5x First Strand Buffer (Life Technologies), 1 μl of 100 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 250 μM Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) dye (Life Technologies, Carlsbad, CA). Cells were then plated onto 35 mm glass bottom microwell dishes that were poly-d-lysine coated (MatTek Corporation ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Neuroscience 2023Quote: Organ of Corti explants were dissected at postnatal day 4 through 7 (P4-P7) in Leibovitz’s L-15 cell culture medium (Invitrogen), containing the following inorganic salts (in mM) ...
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bioRxiv - Cell Biology 2024Quote: Cells cultured for 4 days at a starting density of 7 x 104/cm2 were lysed in TRIzol (Ambion) for 5 min and transferred to an RNA-free microcentrifuge tube following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: ... Raji B cells were labelled in serum-free medium with 10 μM 7-amino-4-chloromethylcoumarin (CMAC, Thermofisher, USA) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... A final concentration of 10 μM Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) dye (Life Technologies; Carlsbad, CA) and 1 μM of MG-TAU dye (cell permeant dye αRed-np1 ...
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bioRxiv - Genomics 2021Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 (Thermo Fisher Scientific) using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar twisted pair platinum-iridium insulated electrodes (MS333/8-BIU/SPC, Plastics One, Roanoke, VA) coated with Vybrant® DiI cell-labeling solution (Invitrogen, Eugene, OR) were placed bilaterally in one of four regions of interest ...
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bioRxiv - Bioengineering 2024Quote: ... The electroporated sample was then transferred directly into one well of an 8-well microscopy slide (Nunc Lab-Tek II for widefield imaging) containing 300 µl prewarmed medium without antibiotics and was incubated for 24 hr in 5% CO2 atmosphere at 37 °C.
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bioRxiv - Microbiology 2021Quote: Vero-6 cells (ATCC CRL-1586) were maintained in DMEM medium (Gibco, 4.5 g/L glucose, w/o pyruvate) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: ... L4-6 ganglia were isolated from adult male Sprague Dawley rats (175-250 g) and dissociated with collagenase (Invitrogen). Cells were washed and then plated with DMEM/F12 (Cellgro ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.5 μg pMD2.G were transfected into HEK293T cells in 6-well plates by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... The chromatin with the antibodies was incubated with 6 μl of Dynabeads Protein G (Thermo Fisher Scientific, Cat# 10003D) for one hour ...
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bioRxiv - Immunology 2020Quote: ... whereby the transfected population is selected for removal of HXGPRT and replacement with the complementation allele in 6-thioxanthine selection medium [177 µg/mL of 6-thioxanthine (TRC, cat# T385800) in 4.5 g/liter D-glucose in GlutaMAX DMEM (Gibco), with 1% dialyzed FBS (Omega Scientific ...
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bioRxiv - Genomics 2024Quote: ... 3 µg of each plasmid was mixed with 6 µl of Plus reagent and 7.5 µl of Lipofectamine LTX (Invitrogen) reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was resuspended in 1 ml of DMEM before adding the mixture to a 6-well plate containing 2 ml of DMEM with 4.5 g/L D-Glucose and L-Glutamine (Gibco) supplemented with supplemented 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 4T1 cells were injected and mice treated with either 1 or 3 doses of 8 mg/kg carboplatin or vehicle by tail vein injection staggered by one day with anti-VEGFR3 antibody (100 μg per injection × 3 total injections, I.P., eBioscience (now ThermoFisher), Control IgG ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative expression was calculated using the ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative genomes were calculated using the ΔCT method ...
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bioRxiv - Immunology 2024Quote: ... One cm pieces of small intestine were then incubated in HBSS containing 3 mM EDTA and 7.5% FBS (ThermoFisher) for 1 hour in a shaker at 37° C ...
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bioRxiv - Genomics 2023Quote: ... One microliter of each cDNA sample was used for TaqMan PCR (Eppendorf RealPlex Mastercycler and Applied Biosystems QuantStudio 3) with 50 heating cycles at 94°C for 30 seconds ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were cultured for 4 days with 1 µM 4-OHT in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with GlutaMax (Thermo Fisher Scientific), non-essential amino acids (NEAA ...
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bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µg/mL Blasticidin S HCl and 1 µg/mL Puromycin Dihydrochloride (Gibco) were used to select for stable transformants.
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bioRxiv - Developmental Biology 2021Quote: ... and quenched 1:5 in DMEM supplemented with 10% FBS (Life Technologies, 10270106). Cells were monthly tested for mycoplasma contamination by PCR.
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bioRxiv - Microbiology 2020Quote: ... grown in DMEM with 5% fetal bovine serum and 1% antibiotic/antimytotic (Gibco). Calu3 2B4 cells were grown in DMEM with 10% defined fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 5 minute incubation in DAPI (1:5,000; ThermoFisher Scientific, Waltham, MA). Slides were then washed ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin (Invitrogen) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: Cells were stained with Nile Red (5◻μg.mL−1 final concentration; Invitrogen) according to Cakmak (2012 ...