Labshake search
Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... and human antibody (bound to phage) was immunoprecipitated using 40ul of a 1:1 mix of protein A/G magnetic beads (Thermo Fisher, Waltham, MA, #10008D & #10009D). Beads were washed 4 times and antibody-bound phage were eluted into 1ml of E ...
-
bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
-
bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
-
bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
-
bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Cell Biology 2020Quote: ... The mEGFP-tagged Tom20 line was transfected with mCherry-Mito-7 construct (Michael Davidson, addgene #55102) using 6 μl per well of transfection mixture containing 25 μl Opti-MEM (ThermoFisher #31985-070), 1.5 μl GeneJuice (Millipore #70967 ...
-
bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
-
bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
-
bioRxiv - Microbiology 2020Quote: ... Reactions were run using primer/probes at 1:20 dilution and Taqman FastVirus one-step reaction mix (Thermo Scientific), and amplified using an Applied Biosystems QS3 cycler ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed three times with PBS and incubated for one hour with secondary antibody (Alexa Fluor 594, chicken anti-mouse 1:1’000, Invitrogen) in PBS with 0.1 % Triton X-100 and 6 % FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... and one or more of the following antibodies: goat anti-rabbit IgG Alexa Fluor 568 (1:1000, #A11036, Invitrogen), goat anti-mouse IgG Alexa Fluor 647 (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... in 0.1%TBS-Tween for one hour and incubated with anti-Biotin (Invitrogen, Cat # 31852, RRID: AB_228243, 1:1000) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for one series included rabbit anti-beta Amyloid (1:300, Cat# 715800, Lot# SH257822; Invitrogen, Waltham, MA). Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500 ...
-
bioRxiv - Immunology 2022Quote: ... After one washing step (1% BSA/PBS) cells were permeabilized in PermBuffer (saponin-based permeabilization and wash buffer; ThermoFisher) for 15min and subsequently stained with pAKT Ser473 (CellSignaling ...
-
bioRxiv - Neuroscience 2020Quote: ... and AMG44 (final concentration: 5μM) treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018], 1% N2 [Gibco, 17502048] ...
-
bioRxiv - Molecular Biology 2021Quote: Cells were lysed by adding one volume of TNES buffer (TNE + 1% SDS) supplemented with RNaseA (Invitrogen cat #12091021) at final concentration of 100 μg/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... 1:2000) and secondary antibody for one hour (Optogenetics experiment: Alexa Fluor 488 goat anti chicken, Invitrogen (cat# A11039), 1:400 ...
-
bioRxiv - Cell Biology 2023Quote: ... incubated for one hour at room temperature (1:1000, Alexa Fluor 488 Goat anti-Mouse IgG, Invitrogen A-11001), and similarly washed ...
-
bioRxiv - Physiology 2023Quote: ... and one of the following secondary antibodies: Donkey anti-rabbit Alexa Fluor 488 (Invitrogen; Catalog# A21206; 1:250 dilution), Donkey anti-goat Alexa Fluor 647 (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... USA) and incubated for one hour at room temperature with Streptavidin Alexa Fluor™ 568 conjugate (1:500)(Invitrogen; Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cellular DNA was extracted with one volume of phenol:chloroform:isoamyl alcohol (25:24:1, #15593031, ThermoFisher Scientific, Waltham, MA, USA), precipitated with one volume of isopropyl alcohol ...
-
bioRxiv - Biophysics 2020Quote: ... The CE was then loaded onto 8 ml prewarmed 7.5% sucrose solution in extraction buffer and microtubules pelleted at 40,000 × g (SS34 rotor, Thermo Fisher Scientific) for 30 minutes at 37 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 25 mg Orange G in 10 mL H2O) was added to each reaction and run on an 8% TBE gel (Invitrogen) (180 V ...
-
bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 72 µg PE-Puro or PE-FeLV plasmid were mixed with 8 µg pCS2-GFP and 40 µl Lipofectamine P3000 (Invitrogen) in 3.2 ml Opti-Mem (Gibco) ...
-
bioRxiv - Neuroscience 2022Quote: ... 50-100 μg of protein in 10-40 μl volume was electrophoresed through 8% or 12% Bolt Bis-Tris Plus gels (InVitrogen) in Bolt MOPS or MES SDS running buffer (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... Transfection was performed with a total of 12 μg of library and 8 μg of VSV-G using Lipofectamine 2000 (Invitrogen) overnight ...
-
bioRxiv - Developmental Biology 2021Quote: ... DAPI staining was performed for 3 mins before mounting with Fluoromount G (Invitrogen, #00-4958-02). The antibodies ...
-
bioRxiv - Bioengineering 2019Quote: ... and 3 μg of pMD2.G were transfected with 60 μl of Lipofectamine 2000 (Life Technologies). Virus was harvested twice at 48 h and 72 h post-transfection ...
-
bioRxiv - Microbiology 2022Quote: ... the mixture was incubated with 3 μl of Dynabeads™ Protein G magnetic beads (ThermoFisher Scientific). After 1 hr rotation at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: WEHI-3 cells were cultured in DMEM (4.5 g/L glucose, 110 mg/L pyruvate; Gibco) supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Immunology 2024Quote: ... WEHI-3 cells were cultured in DMEM (4.5 g/L glucose, 110 mg/L pyruvate; Gibco) supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Immunology 2020Quote: ... 000 x g at 4°C and protein concentration measured using Pierce BCA (Thermo Fisher). Equivalent amount of protein in each sample were immunoprecipitated by rotating with 0.25-1 µg of FADD antibody (M19 ...
-
bioRxiv - Plant Biology 2024Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
-
bioRxiv - Plant Biology 2023Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sonicated material was then pooled together and distributed into 500 μL aliquots (equivalent of 7*108 cells per aliquot) and 25 μl of Protein G magnetic beads (Life Technologies-Invitrogen) pre-bound with 6μg ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested at different timepoints during co-culture (0h, 6h, 12h, 24h) and washed in cold 1X DPBS (Gibco Carlsbad, California, USA). Harvested cells were stained with primary anti-TfR1 antibody (ab84036 ...
-
bioRxiv - Developmental Biology 2023Quote: ... For RNAi YAP and TAZ knock down – hMSCs were starved for 6h in DMEM without antibiotics before adding siRNA for YAP or TAZ and scramble control (Stealth RNAi Invitrogen Thermo Fisher Scientific) in OptiMEM with addition of Lipofectamine 3000 (both Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: ... then four times in PBS for 5 minutes (5 minutes for each wash) before mounting with Floromount G (Thermo Fisher Scientific). Slices were imaged an Olympus VS120 slide scanning microscope ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 40 μl of solubilized protein lysate was stained with 5 μl of 5% G-250 sample additive (Thermo Fisher Scientific). Wells of a Native-PAGE 3–12% Bis-Tris protein gel were washed with the dark blue cathode buffer ...
-
bioRxiv - Immunology 2022Quote: ... BMDMs were seeded into 6-well plates (one million per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 25 mM unlabeled glucose ...
-
bioRxiv - Genomics 2020Quote: ... 10 ng of the library were transfected into 250 000 HEK293 cells in one well of a 6-well plate using Lipofectamine 2000 (11668027, ThermoFisher Scientific) and OPTIMEM I Reduced Serum Medium (31985-047 ...
-
bioRxiv - Cell Biology 2021Quote: ... One microgram of siRNA was transfected in one well of a 6-well using Lipofectamine RNAiMAX transfection reagent according to the manufacturer protocol (Thermofisher Scientific). Cells were treated with siRNA for 24h.
-
bioRxiv - Genetics 2023Quote: Whole cell extracts of 2D cultures from one well of a 6-well plate were prepared using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... Monocyte-derived macrophages (MDM) were generated by seeding three million CD14+ cells into one 6-well plate (NuncTM Thermo Fisher Scientific) in IMDM (Thermo Fisher Scientific ...