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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...
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bioRxiv - Cell Biology 2021Quote: ... The tissues were then digested in room temperature for 7 min in a 3-mL Hank’s solution containing 0.25% Trypsin (Thermo Fisher, 15090046) and 0.1 μg/μL DNase (Sigma D5025 ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using SYBR green reagents on an Applied Biosystems QuantStudio 3 and ViiA 7 Real-Time PCR Systems (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Physiology 2021Quote: ... JC-1 dye was added 10 min prior to recording (ex.488/em.535 and em.590) while CellEvent Caspase 3/7 (ThermoFisher) was added immediately prior to recording (ex.495/em.540).
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
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bioRxiv - Cancer Biology 2024Quote: ... apoptosis was evaluated in NCI-H1975 or HeLa cells using CellEvent Caspase-3/7 Green ReadyProbe Reagent (Invitrogen, Cat#R37111) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: 1 μg of cDNA mixed with 1μl PLUSTM reagent were transiently transfected into DIV 3-7 cultured neurons by Lipofectamine™ LTX (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Neuroscience 2024Quote: ... 3% dilution, 4 µl,), Fluoro-ruby (FR, case BC, (10% solution, mixture of 10 kDA and 3 kDA, 4 µl; Invitrogen), and Biotinylated Dextran Amine (BDA ...
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bioRxiv - Developmental Biology 2021Quote: ... These lines were routinely passaged every 3-4 days using Versene (ThermoFisher; 15040066). All lines were routinely screened for differentiation and tested for mycoplasma contamination.
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bioRxiv - Biophysics 2022Quote: ... DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate salt) was purchased from ThermoFisher scientific (Molecular probes ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781 ...
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bioRxiv - Neuroscience 2020Quote: ... 3-4 brains were dissected in chilled Schneider’s Drosophila medium (ThermoFisher Scientific, 21720001) in less than 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... that accumulates in hyperpolarized membranes and DiBAC(4)3 (Thermo Fisher Scientific, USA) that enters depolarized cells (Suchodolski and Krasowska ...
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bioRxiv - Developmental Biology 2021Quote: ... and passaged every 3 – 4 days using Versene (Cat. # 15040066, Thermo Fisher Scientific). Culture dishes were pre-coated with 0.5% GelTrex matrix solution (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were split every 3-4 days when confluent using TrypLE Express (Gibco). For all immunoblotting and imaging experiments ...
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bioRxiv - Neuroscience 2023Quote: ... 3/4 of the medium was replaced with Neurobasal medium (GIBCO, 21103-049) containing B27 and glutamax ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 3-4 days using TrypLE Express (ThermoFisher, Cat# 12605036) and confirmed to be free of mycoplasma ...
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bioRxiv - Neuroscience 2023Quote: ... After 3–4 days the colonies were dissociated using StemPro Accutase (Thermo Fisher), counted and plated at low confluency (10,000 cells/cm² ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were loaded with 3 µM Fluo-4 AM (ThermoFisher Scientific cat# F14201) diluted in basal media for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cells were passaged every 3-4 days using 0.05% Trypsin-EDTA solution (Gibco).
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bioRxiv - Genetics 2021Quote: ... embryos were stained with 1 μg/ml DAPI and mounted with Fluoromount-G (ThermoFisher Scientific, Waltham, MA). Images were taken as described above under immunofluorescence.
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bioRxiv - Immunology 2020Quote: ... Cells were cultured in complete DMEM (4.5 g/L D-Glucose, L-Glutamine, 1% penicillin/streptomycin, Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a 1 h-incubation with 100 μL protein A/G agarose beads (Thermo Scientific Pierce) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 1 g of each sample was added to 10 mL sterile UltraPure water (Thermo Fisher, Waltham, MA) in a 50 mL conical Tube (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... and secondary antibody used was Alexa Fluor 488 goat anti-rabbit immunoglobulin G (IgG) (1:200, Invitrogen).
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bioRxiv - Immunology 2022Quote: ... cDNA was prepared from 0.4-1∼g of total RNA using SuperScript IV reverse transcriptase (ThermoFisher Scientific). Quantitative PCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... containing 1.0 g l−1 glucose and 10% fetal bovine serum (Gibco–Life Technologies, Carlsbad, CA, USA). Medium for the WT cell line contained 1% Geneticin G418 (A&E Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... containing 1.0 g l−1 glucose and 10% fetal bovine serum (Gibco–Life Technologies, Carlsbad, CA, USA). Medium for the WT cell line contained 1% Geneticin G418 (A&E Scientific ...
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bioRxiv - Biochemistry 2021Quote: HEK293T cells were cultured in growth medium (DMEM, Glutamax, 4.5 g Glucose, 10% FCS, 1% PS; Invitrogen) at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: Total IgG was isolated from 1 mL plasma using Protein G Plus Agarose (Pierce Thermo Fisher Scientific) affinity chromatography and cleaved into F(ab’)2 fragments using IdeS ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µ g of the resulting RNA was reverse transcribed using SuperScript III reverse transcriptase (Invitrogen). Equal volumes of cDNA were loaded for each qPCR reaction ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was prepared from 1 g RNA using a high-capacity RNA-to-cDNA kit (Applied Biosystems), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated by retrotranscription of 1 g of total RNA using M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... TET21-N cells were routinely maintained in medium containing 0.2 mg ml−1 G-418 (11811031, GIBCO) and 0.15 mg ml−1Hygromycin B (10453982 ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: Alexa Fluor 488 donkey anti-mouse immunoglobulin G (1:200, Thermo Fisher Scientific; A-21202) and Alexa Fluor 555 donkey anti-rabbit immunoglobulin G (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were stained with DAPI (1:5000) and coverslips mounted on glass slides using Fluoromount-G (Invitrogen). Three-five different regions per coverslip were imaged on a Zeiss LSM710 confocal microscope using the LSM Pascal 5.0 software.
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bioRxiv - Neuroscience 2024Quote: ... The concentrated proteins were supplemented with Dulbecco’s modified Eagle’s medium 1 g/L glucose (DMEM; Gibco, Invitrogen) with 2 mM L-glutamine (PAA ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrated proteins were supplemented with Dulbecco’s modified Eagle’s medium 1 g/L glucose (DMEM; Gibco, Invitrogen) with 2 mM L-glutamine (PAA ...
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bioRxiv - Biochemistry 2023Quote: ... OECs were then centrifuged at 5000 x g for 1 min and resuspended in RNAlater (Applied Biosystems) and stored at -20°C until RNA extraction ...
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bioRxiv - Bioengineering 2023Quote: ... F actin and G actin of the cells were stained using phalloidin-AF488 (1:400 dilution, ThermoFisher), DNAse I AF594 (9 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... USDA 110 was cultivated in PSY medium supplemented with L-(+)-arabinose (1 g/l) (Thermo Scientific A11921) [34] ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were diluted to less than 1 ×106 cells/mL in PBS with 3% FBS and incubated in 1:100 EpCAM-APC (Invitrogen, 17-5791-80) or 1:100 IgG-APC isotype control (BioLegend ...