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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: All transfections were performed on 293T cells (seeded at a density of 0.5 × 106 cells/well of a 6-well plate one day prior) using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendation unless stated otherwise ...
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bioRxiv - Neuroscience 2023Quote: Aggregates from four wells of a 6-well plate were transferred to one 175 cm2 tissue culture flask (Thermo Scientific, #159926) previously treated with an anti-adherence solution (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... 5’/VIC/CCTTGGGTTCTTGGGA/3’/MGB (Thermo Fisher Scientific; (Bruner et al., 2016)) ...
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bioRxiv - Immunology 2020Quote: ... 5’/NED/AAGCCAGGAATGGATGGCC/3’/MGB (Thermo Fisher Scientific; (Schmid et al., 2010)).
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bioRxiv - Microbiology 2021Quote: ... Sections were cut at 3-5 μm on a cryotome (ThermoFisher Scientific) using C35 carbon steel blades (Feather ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 5 μl Turbo DNase buffer and 3 μl Turbo DNase (ThermoFisher Scientific) were added to each reaction and incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was suspended in 3-5 ml TrypLE Express (ThermoFisher, 12605028) depending on the pellet volume and incubated at 37°C for 10-15 min with mixing every 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% (for RS-5) or 20% (for DM-3) FBS (Gibco). All the other cells were cultured in RPMI medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged every 3-5 days using Trypsin-EDTA (Gibco, 25200056). All experiments were done with cells at 10 passages or earlier with regular testing for mycoplasma ...
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bioRxiv - Cell Biology 2020Quote: ... 5′-ATTTGTCGACTCATTCTAATCCTTCGTCTTTTGATT-3′ by using Phusion high-fidelity DNA polymerase (Thermo Scientific) and cDNA prepared from the parasites as template ...
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bioRxiv - Cell Biology 2020Quote: ... were transfected with human myosin VI siRNA duplex (5′GGUUUAGGUGUUAAUGAAGtt-3′) (Ambion) or AllStars Negative Control siRNA duplex (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... LUVs were prepared using sonication (Fisher Scientific, Ultrasonic Bath, 3 × 5 min). Sonication was performed in ice water bath ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl Turbo DNase buffer and 3 μl Turbo DNase (ThermoFisher Scientific) were added to each sample and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... TOGARAM1 was depleted using siRNA with sequence 5′-CCUCGUAAUUCCUUAGAAA-3′ (Thermo Scientific) Cells were seeded onto coverslips at 70% confluency and transfected with 50 nM of siRNA in two sequential transfections using Lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... ID: s2513 (Silencer Select Validated; 5’-GA UAUACCCUGGAAAGUCUtt-3’) (Thermo Fisher Scientific) with JetPrime (Polyplus ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was quantified using a Nanodrop One-One (Thermofisher) before cDNA amplification or RNA seq libaries preparation.
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bioRxiv - Cancer Biology 2021Quote: ... 0.5-1×104 cells per well were seeded in 6-well Falcon plates (Fisher Scientific, Cat #087721B) in triplicate ...
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bioRxiv - Genomics 2022Quote: ... Cells were routinely passaged 1:6 once they reached 75% confluency using 0.25% trypsin-EDTA (Gibco, 25200056) for HEK293T cells ...
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bioRxiv - Neuroscience 2022Quote: ... Huh7 or SH-SY5Y cells in 6-well plates were incubated with 1 μM puromycin (Thermo Fisher) for 10 min at 32°C (STHdh ...
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bioRxiv - Genetics 2022Quote: ... A mix of 6 ul of nick translated RNA probe with 12ul of Human Cot-1 (ThermoFisher) and 2ul of salmon testes (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... 1 filter paper for ~6 seconds with blot force 0 on a Vitrobot Mark IV (ThermoFisher Scientific) at 100% humidity and 22°C.
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bioRxiv - Cell Biology 2024Quote: ... Overexpressed SAS-6 was detected with mouse anti-c-Myc (1:250; 9E10; Invitrogen, UK; 13-2500) antibody ...
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bioRxiv - Immunology 2019Quote: ... neutrophils were loaded with 5 μM Fluo-4 AM (Thermo Fisher) and incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Excess cells were washed off with PBS (4×5 mL, Gibco), briefly left in RPMI (5 mL ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were loaded with 5 μM fluo-4 AM (Invitrogen) in Hanks’ balanced salt solution (HBSS ...
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bioRxiv - Cell Biology 2023Quote: ... 4-5 μl Page Ruler Pre-stained Protein Ladder (Thermo Scientific), and 10 μl of 4X Dye (last three grooves ...
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bioRxiv - Cancer Biology 2024Quote: ... Excess cells were washed off with PBS (4×5 mL, Gibco), briefly left in RPMI (5 mL ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of T4 DNA ligase (Thermo Fisher, 5 WU/µl) was added ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Cell Biology 2022Quote: ... and cultured for 5-7 days at P0 before been passaged with Tryspin - EDTA 0.05% (Thermo Scientific, 25300054). Cells were passaged every 4-5 days and until p8 ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa and COS-7 cells were seeded onto dishes coated with 5 mg fibronectin (Life Technologies 33016-015) in diH2O ...
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bioRxiv - Immunology 2024Quote: ... were cultured for up to 7 days at 37°C in 5% CO2 in RPMI 1640 media (Gibco) supplemented with 10% Heat Inactivated FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Immunology 2020Quote: ... samples were loaded under non-reduced conditions on a precasted 3-8% Tris-Acetate SDS-PAGE gel (Invitrogen). Proteins were transferred to a nitrocellulose membrane using an iBlot2 dry blotting system (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Up to 10μg of total protein was resolved on NuPAGE™ 3-8% Tris-Acetate Protein Gels (Invitrogen) in 1x NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded onto a NuPAGE® Novex® 3–8% Tris-Acetate (Thermo Fisher, Waltham MA, USA) and run at 100V during 5h ...
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bioRxiv - Biochemistry 2021Quote: ... were centrifuged at 16000 g for 30 min and the concentration of peptides were measured by Nanodrop one Microvolume UV-Vis Spectrophotometer (Thermo Fisher). All peptide samples were analyzed in technical triplicate by LC-MS using an Easy-nano LC 1200 system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... About 8-15μg of total protein extract was resolved on NuPAGE 4-12% Bis-Tris gels (Invitrogen) using MES SDS running buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... 8-25 μm of protein per lane was resolved in 4-12% NuPAGE Bis-Tris gels (Invitrogen) and chemiluminescent quantitation was performed as previously described (23).
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bioRxiv - Molecular Biology 2019Quote: ... Boiled samples were loaded into 8% SDS-PAGE or NuPAGE 4% - 12% Bis-Tris protein gels (ThermoFisher). Specific protein targets were analyzed by immunoblot assay with specific antibodies listed in Table ...
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bioRxiv - Microbiology 2024Quote: ... GECs (8 x 104 cells) were seeded in 4-well chamber slides (Fisher Scientific# 12-565-2) and pre-treated with 2 mM Glu-Glu for 1 h prior to infection with wild-type or ΔgdhA strains of P ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...