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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher, W32464, diluted 1:40) and donkey a-rabbit-AF488 (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng cDNA in a 20 μL reaction and primers at 1 μM final concentration on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). Primer specificity was ensured by designing primers to span exon-exon junctions ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen appendix tissue (∼20 mg) was homogenized using a Covaris cryoPREP pulverizer and then in 1 mL of TRIzol (Life Technologies) with a ceramic bead-based homogenizer (Precellys ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg/ml in sterile water Gibco 10977035, 1 hour at 37°C followed by 3 washes with PBS Gibco 10010015) / Laminin (Gibco 23017-015 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a 20 minutes incubation with the appropriate secondary antibodies coupled to a fluorochrome Alexa 555 (1:500, Life technologies). Cells were washed three times in PBS and once in water and mounted onto slides with Prolong™ Gold Antifade Mountant with DAPI (Molecular probes ...
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bioRxiv - Cancer Biology 2020Quote: ... The eluted polyA+ RNA was ethanol precipitated and resuspended in 10μL of DEPC treated water with 1:20 SuperaseIN (Life Technologies, USA). Double-stranded cDNA was synthesized from the purified polyA+ RNA using the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2020Quote: ... The samples were incubated (10 min, 20°C, in the dark) with 1 μM (final concentration) of SYTOX Green (Molecular Probes). SYTOX Green binding to bacterial DNA was measured using a microplate reader (Molecular Devices SpectraMAX GeminiXS ...
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bioRxiv - Microbiology 2020Quote: ... Devices were washed twice with PBS followed by one rinse with 3% NGS plus 0.1% Tween-20 for 5 min each with rocking and secondary antibodies Alexa Fluor 488 goat anti-mouse IgG (Thermo Fisher) and Alexa Fluor 555 goat anti-rabbit IgG (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The buffer with dissolved gel slice was added to 1 mL ice-cold 100% ethanol and 2 μL of glycogen (20 mg/ml, ThermoFisher, R0561) and incubated at −20°C for at least one hour ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:20 and subjected to semi-quantitative real time PCR using the SYBR Green reagent (Life Technologies) and specific primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 20% Fetal Bovine Serum and 1% Penicillin-Streptomycin solution (all from Gibco, Thermo Fisher Scientific). The IM-WT-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 20% Fetal Bovine Serum and 1% Penicillin-Streptomycin solution (all from Gibco, Thermo Fisher Scientific). Primary cell cultures PCB-00007 ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then washed three times for 15 minutes in 0.1 % Tween-20 in PBS and incubated in PEMBALG containing monoclonal anti-rat antibody conjugated with Alexa488 (1:1000 dilution, Life Technologies) for one hour ...
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bioRxiv - Genomics 2020Quote: ... This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s using the QuantStudio 5 Real-Time PCR System (Applied Biosciences, ThermoFisher Scientific). Specificity was verified by melt curve analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was stained in 20 ml of 1 x TBE buffer containing 2 μl of SYBR Gold stain (ThermoFisher Scientific) for 5 minutes at room temperature and a band migrating just above the 35 nt oligo was cut from the Ribo-seq sample lane.
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were treated for 20-60 s with Proteinase K (1 μg/ml) in TE buffer (Invitrogen – Thermo Fisher Scientific). Single nNOS (+ ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: ... cells were incubated with 20-50 nM SiR-tubulin dye (SpiroChrome) for 1-2 hours and transferred to L-15 medium (ThermoFisher Scientific) before imaging ...
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bioRxiv - Immunology 2022Quote: We homogenized nasal turbinates and lung tissue samples in 1:20 (w/v) of tissue protein extraction reagent (Thermo Fisher, # 78510), then centrifuged them to pellet tissue debris ...
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bioRxiv - Microbiology 2021Quote: ... and incubation at 37°C for 1 hr and 2) 1 hour incubation at 50°C following addition of 15μl Proteinase K (20 mg/ml, Thermo Scientific™) and 30μl Proteinase K digestion buffer (0.1M NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Viable splenocytes were co-cultured with the indicated tumor cell line at a ratio of 1:20 (tumor cell line: splenocyte) in the presence of anti-CD3/ anti-CD28 (5 μg/ml) (Life technologies) for 96 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed once with TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 1% Tween 20) and incubated with polyclonal antibodies (Thermo SCIENTIFIC, USA) specific for IFIT1 (1:1000) ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton for 20 minutes at room temperature and labeled with Alexa Fluor 647 using the Click-iT reaction (Thermo Fisher) for 30 minutes according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were normalized to optical density at 600 nm (OD600)=1 and centrifuged at 100xg for 20 minutes at 22 °C (Marathon 3000R, swinging bucket, Fisher Scientific). OD600 of supernatant was determined (BioMate 3 Thermo Spectronic ...
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bioRxiv - Systems Biology 2022Quote: ... and then stained the cells for 20 min with 1 µg / mL of propidium iodide (Thermo Fisher Scientific, cat. No. P3566). We then flowed these (stained ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was then added to be a 20 μL reaction and incubate at 50°C for 1 hour to generate cDNA (SuperScript III, ThermoFisher Scientific). The HA1 sequence was divided into three fragments for sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% Tween-20 in PBS) for 10 minutes and incubated with 1:400 diluted AlexaFlour 594 secondary antibody (Invitrogen A-11007) in blocking solution for 2 hours at RT in the dark ...
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bioRxiv - Biochemistry 2019Quote: ... and P fractions was measured by BCA assay by a dilution of 1:20 in PBS according to the manufacturer’s instruction (Thermo Fisher Scientific).
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bioRxiv - Physiology 2020Quote: ... Then sections were incubated in the dark for 20 min with Texas red-WGA (1:50) conjugate (Invitrogen, Breda, The Netherlands) and washed with PBS ...
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bioRxiv - Immunology 2020Quote: ... in PBS/0.5% BSA at room temperature for 2 h and washed in PBS/0.2% Tween-20 at room temperature for 1 h five times before mounting with SlowFade Diamond Antifade Mountant (S36963, Life Technologies).
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bioRxiv - Neuroscience 2019Quote: ... for 80 minutes (4 × 20 min) at room temperature before secondary antibodies (donkey anti-rabbit Alexa Fluor 1:1000, Life Technologies Cat No ...
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bioRxiv - Cancer Biology 2020Quote: ... stained at 37°C for 20 minutes with 1 μM (final concentration) of the CellTrace Far Red reagent (CAT# C34572, ThermoFisher Scientific). After incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with an anti-CD63 primary antibody (1:20 dilution, Thermo Fisher Scientific, cat. # MA1-19281) in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... then 1 mL of a - 20°C pre-chilled precipitation solution was added (50% v/v acetone [A949-4; Fisher Scientific, Fair Lawn ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNAs were diluted 1:20 before relative quantitation of transcript levels by real-time PCR using SYBR Green master mix (Applied Biosystems) and target-specific primers (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... was added at a 1:200 dilution and incubated with cells for 1 h followed by a 20 min incubation with 5 μg/mL Hoechst 33342 (Invitrogen, H1399). HLMVECs were stained for actin with phalloidin conjugated with Alexa Fluor 488 (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Physiology 2019Quote: ... Approximately 20-40 mg of human ventricular myocardium was homogenized in 1 mL of Trizol (Life Technologies #15596-026, Carlsbad, CA) with a TissueLyser LT (Qiagen N.V ...
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bioRxiv - Cell Biology 2021Quote: ... The ganglia were washed twice by pelleting at 800g for 3 min and resuspending in 5 mL ‘HBSS+H’ then enzymatically digested by resuspension in 1 mL 37°C HBSS supplemented with 15 μL 20 mg/mL collagenase type IV (Thermo Fisher). After 1 hour incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were resuspended at a concentration of 2.5-5 × 106 cell/mL in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (F14201, Thermo Fisher Scientific) and 3 μM Fura Red (F3021 ...
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bioRxiv - Cell Biology 2021Quote: ... 6 ng/well) and shRNA-containing transfer (pLKO.1-shRNA, 50 ng/well) plasmids were combined in 20 μl/ well OptiMEM (Life Technologies) containing 0.2 μg/well linear polyethyleneimine 25,000 (PEI ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μL was injected with a split mode 1/20 at 260°C on a TG-5MS column (26098-1420, Thermo Scientific). Separation was performed in a Helium gas-stream at 1 mL.min−1 using the following temperature ramp ...
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bioRxiv - Cell Biology 2022Quote: ... WGA Alexa Fluor 488 conjugate (1:300 dilution in PBS, 20 min incubation in the dark at RT; Thermo Fisher Scientific) was used to stain the plasma membrane ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were washed 4×20 minutes with PBST and then incubated for 30 minutes in Hoechst (1:2000; Thermo Fisher H3570). Samples were washed a final time in PBST and were stored in PBST until imaged.
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bioRxiv - Immunology 2022Quote: ... and 250 U/mL DNase-I) in tumor digestion medium (RPMI-1640 containing 10 mM HEPES, 1% penicillin/streptomycin and 20 μg/mL gentamicin; Gibco/Invitrogen) in a humidified incubator at 37°C with 5% CO2 with gentle rotation for 2-3h ...
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bioRxiv - Molecular Biology 2023Quote: Purified RBPMS-A was exchanged to image buffer (20 mM CAPS, KCl, 1 mM TCEP and pH 10) using a Zeba spin desalting column (7K MWCO, ThermoFisher Scientific). The protein was diluted such that the final concentration was as indicated ...