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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Bioengineering 2022Quote: ... 20 ng/ml Oncostatin M and 1% (v/v) MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalogue no. 11140050), 10 uM DAPT for another 10 days with the replacement of media every 48h ...
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bioRxiv - Cancer Biology 2023Quote: ... Then cells were permeabilised with 0.1% Triton X-100 in PBS (20’, RT) and actin cytoskeleton stained (10’, RT) with phalloidin (165nM final concentration, Thermo Fisher), prior to imaging on LSM 980 confocal laser-scanning microscope equipped with an Airyscan 2 module (Zeiss ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of the microbial cell suspension obtained was stained with 1 μL SYBR Green I (1:100 dilution in dimethylsulfoxide; shaded 20 min incubation at 37 °C; 10,000 concentrate, Thermo Fisher Scientific). The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then washed with 0.1 M PBS (6×20 minutes at 4°C) and incubated with secondary antibodies conjugated to Cy3 and Alexa Fluor 488 (1:500, ThermoFisher, Germany) in 0.1 M PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/ml BDNF and 10 ng/ml GDNF medium supplemented with 0.6 μl ethidium homodimer-1 594 (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted CD31-CD45-CD184+ cells were cultured in complete growth medium DMEM supplemented with 20% FCS and 1% penicillin/streptomycin (Gibco, 15140122).
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bioRxiv - Molecular Biology 2022Quote: ... 1% sodium deoxycholate, 0.1% SDS), supplemented with phosphatase inhibitors (10 mM NaF, 20 mM β-glycerophosphate) and protease inhibitor (Thermo Scientific) at ∼106 cells/mL and incubated on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Total biotinylated proteins in samples were detected with Alexa Fluor™ 790 Streptavidin Conjugate (1:20 000, S11378, Thermo Fisher Scientific). Band densities were quantified using Image Studio quantification software (LI-COR Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... 2.5 million iPSCs were treated with accutase and electroporated using the Neon transfection system (100 μl tip; 1600 V Voltage, 20 ms Width, 1 Pulse; Life Technologies) with 2 μg of AAVS1 ZFN plasmid and 6 μg of the modified AAVS1 donor plasmid ...
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bioRxiv - Microbiology 2023Quote: ... F and G in Figure 1 titer was determined using a PA-HPLC analysis using a POROS A 20 μm column (Applied Biosystems) on an Agilent 1260 system ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were blocked with 1% BSA for at least 20 min and treated with Avidin Alexa Fluor 350 (Invitrogen Molecular Probes) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... approximately 150 brains of 3-to-5-day-old male flies were homogenized in 300 μl of extraction buffer (20 mM Tris pH 7.6, 50 mM NaCl, 1 % Triton X-100, 1XHalt Protease inhibitor Cocktail (Thermo Fisher, 78429)) ...
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bioRxiv - Immunology 2023Quote: ... 5 ml of the total nucleic acid eluate was added to a 20-ml total-volume reaction mixture (1x TaqPath 1-Step RT-qPCR Master Mix, CG [Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then washed in PBST and counterstained for 20 minutes with 1 μM DAPI and mounted under coverslips with Prolong Glass (Thermo Fisher). Representative tumor areas were imaged with a 20x objective in an Agilent Cytation 5 imaging system ...
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bioRxiv - Genetics 2023Quote: ... The obtained pellet was resuspended in 1 mL of NWB and filtered through a pre-wet 20 µm CellTrics filter (Fisher Scientific). Nuclei were finally centrifuged at 500 g for 5 minutes and resuspended in 200 µL of NWB buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µL of DNA lysate was added to a 20 µL amplification reaction (Phusion Plus Green PCR Master Mix, ThermoFisher, F632L). 400-600 bp fragments flanking the edited regions were amplified ...
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bioRxiv - Neuroscience 2023Quote: 1 µl cDNA was used for qPCR in a volume of 20 µl using TaqMan GeneExpression Master Mix (Thermo Fisher Scientific) according to manufacturer’s instructions using a StepOne Plus Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times with PBS for 20 mins each then incubated for one hour in appropriate fluorescently-conjugated secondary antibodies (1:500, ThermoFisher Australia). Brainstem sections were kept in order and mounted onto gelatin-coated slides ...
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bioRxiv - Neuroscience 2023Quote: ... We rinsed 8 times 20 min in PBT3 at RT before an overnight incubation in 1:500 goat anti-rabbit AlexaFluor 633 (Invitrogen A21070) and 1:500 goat anti-mouse AlexaFluor 488 (Life Technologies A11001 ...
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bioRxiv - Microbiology 2023Quote: Bacteria cells and meropenem in liquid media are briefly vortexed before mounting on a 1% (v/v) UltraPure agarose pad (10 x 20 mm, Invitrogen US) in M9 minimal media (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 5 ml of the total nucleic acid eluate was added to a 20-ml total-volume reaction mixture (1x TaqPath 1-Step RT-qPCR Master Mix, CG [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1X MOPS (20 mM MOPS, 5 mM NaOAc, 1 mM EDTA, pH 7.0) and 1X RNA loading dye (Thermo Fisher Scientific) for 10 min at 70°C and incubated on ice ...
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bioRxiv - Microbiology 2023Quote: ... coli (OD600nm ∼ 1.0 Abs) cultured in LB was mixed with propidium iodide (1:1000 diluted from 20 mM stock solution, Thermo Fisher Scientific) and anhydrotetracycline at a final concentration of 500 ng/mL (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed with PBST (PBS, 0.1% Tween-20) and incubated with Alexa Fluor-conjugated secondary antibody (1:500, Thermo Fisher Scientific) and DAPI (1 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... DNA staining was performed using Hoechst 33342 at a 1:2000 dilution of a 20 mM stock solution (Cat # 62249, ThermoFisher Scientific). The cells were mounted onto microscope glass slides (Lomb Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were blocked with 1% BSA for at least 20 min and treated with Avidin Alexa Fluor 350 (Invitrogen Molecular Probes) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... RT-PCR reactions were performed in a 20 μL mixture containing 1× Power SYBR™ Green PCR Master Mix (Thermofisher Scientific), 0.5 μmol/L of each primer ...
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bioRxiv - Neuroscience 2023Quote: ... repair template (Supplemental Table 1) were introduced into ARPE-19 cells using electroporation (1400 V, 20 ms, 2 pulses, Neon Transfection System, Life Technologies). After electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mg total RNA was used for each 20 ul reverse transcription reaction using SuperScript III RT enzyme (Life Technologies) followed by PCR reactions using the GoTaq Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... and 24 hours after centrifugation and then stained them with 2µM ethidium homodimer 1 (EthD1) in complete cortical media and 20 µg/mL Hoechst (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were collected by centrifugation and washed with inhibitor-containing lysis buffer 5 times before being mixed with 20-40 μl 1× Laemmli sample buffer (diluted from Invitrogen, NP0008) supplemented with β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were resuspended in 0.1% Tween-20 in PBS at the following dilutions: anti-mouse AF488 (Invitrogen, A21131, 1:200), anti-mouse AF647 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... all media was supplemented with heat inactivated foetal calf serum (FBS, 10% McCoy’s 5A and DMEM GlutaMAX; 20% EMEM) and 1000 U/ml (1%) penicillin-streptomycin (Gibco Life Technologies). Cells were incubated at 37°C with 5% CO2 and were not used beyond passage 20.
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl, 0.08% Triton X-100, 25% glycerol, 20 mM reduced glutathione, 1 mM DTT, and 1X synthetic block [Invitrogen cat# PA017]) for one hour with gentle rocking ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Pathology 2024Quote: ... pH 7.4 and 500 mM NaCl, 20 mM imidazole, 1 mM PMSF, protease inhibitor cocktail Set III, EDTA free from Thermo Fisher) and lysed by an LM20 microfluidizer (15000 psi) ...
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bioRxiv - Microbiology 2024Quote: Tissues were dissected from 5-day-old females in RNase free PBS with 0.05% Tween 20 and homogenized in 1 mL of TRIzol (Thermo Fisher Scientific) and stored at -80°C (n = 20 per condition and per independent experiment) ...
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bioRxiv - Biophysics 2024Quote: Vero cells (ATCC CCL-81) were cultured in 35 mm confocal dishes (D35C4-20-1-N) with DMEM (Gibco, Carlsbad, CA) supplemented with 5% FBS (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips, 100-μl bed; Thermo Fisher Scientific). Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg ...
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bioRxiv - Microbiology 2024Quote: ... The human monocytic leukemia THP-1 cell line was thawed and cultured in RPMI media supplemented with 20% FBS and 2-mercaptoethanol (Gibco: 21985023) to a final concentration of 0.05 mM at 37°C in a humidified atmosphere at 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with lysis buffer (20 mM Tris, 300 mM NaCl, 10 mM imidazole, 1 mM PMSF, 1x EDTA-free Pierce Protease Inhibitors (Thermo Scientific), ∼25 ug/mL DNAse I ...
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bioRxiv - Biochemistry 2024Quote: ... which was then passed three times through a 20 mL gravity column packed with 1 mL PierceTM Anti-HA Agarose (ThermoFisher Scientific) equilibrated in binding buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was blocked with 5% nonfat dry milk in TBST with 0.05% Tween 20 for 1 hour at room temperature and then probed with anti-V5 (#R960-25, Thermo Fisher Scientific) and anti-b-Actin (#GTX629630 ...