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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Bioengineering 2024Quote: ... the plates were incubated with horseradish-peroxidase conjugated goat anti-mouse IgG (diluted 1/5,000) secondary antibodies (ThermoFisher 62-6520) for 1 h at room temperature ...
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bioRxiv - Biophysics 2024Quote: Fluorogenic POP substrate Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin (AMC) (HANGZHOU JHECHEM CO LTD) was diluted in PBS to 1-25 µM final concentrations in 96-well plates (ThermoFisher) to measure enzyme activity parameters in real time and as end-point reactions at 10 and 30 min ...
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bioRxiv - Neuroscience 2024Quote: Neuro2A-cells (ATCC #CCL-131) were cultured in six-well plates (106 per well) in DMEM + GlutaMAX-1 (25300-054/096, GIBCO by Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... IgGs from individual serum or pooled sera bound to the plates were detected after a 2-h incubation with goat anti– mouse-IgG–linked horseradish peroxidase (1:8,000 Invitrogen, USA). For measuring IgG isotypes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were grown in µ-Slide 8 well high ibiTreat (80806 Thistle Scientific) or 96-well plates to approximately 60% confluency and preloaded with 1 µM Fluo4-AM (F14201, Invitrogen) /0.02% Pluronic® F127 in HBSS ...
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bioRxiv - Developmental Biology 2024Quote: ... Five droplets were added to a 12-well plate and covered with 1 mL of TOM [Advanced DMEM/F12 (Gibco), 1x N2 supplement (Gicbo) ...
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bioRxiv - Immunology 2024Quote: ... plates were rewashed and incubated with HRP-conjugated goat anti-hamster IgG (H+L) secondary antibody (HA6007; Invitrogen; 1:1500) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15 µL of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15ul of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Tissue was added to 96-well round bottom plates in 100μL of R10 (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, 1X Glutamax (Thermo Scientific), and 55μM b-mercaptoethanol) ...
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bioRxiv - Immunology 2023Quote: 293T IFNB12X-GLuc cells plated in 96-well plates were transfected with 1 pmol/well siRNA (Silencer Select, Thermo Fisher) using Lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were thawed onto PLO and laminin-coated plates and cultured in astrocyte media supplemented with 1% knockout serum replacement (KSR)(Thermofisher). Astrocyte progenitor cells (APCs ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM EDTA pH 8.0) and purity was assessed using the Multiskan SkyHigh Microplate Spectrophotometer and µdrop plate (Thermo Scientific). Sample purity was assessed by measuring the 260/280 nm and 260/230 nm absorption and only samples achieving 1.7-2.0 and 2.0-2.2 respectively were further evaluated.
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bioRxiv - Microbiology 2023Quote: ... stained bacteria were fed to ciliates using a 1:200 protist:bacteria ratio and transferred to a 96 well black plate (Thermo Scientific). DCF fluorescence was monitored automatically by measuring green fluorescence with 480 nm excitation and 512-552 nm emission wavelengths with a TECAN Infinite MPlex plate reader every 15 min ...
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bioRxiv - Genomics 2023Quote: ... EBs were plated on polyornithine/laminin-coated plates and cultured in N2/B-27 media containing 1 μg/mL laminin (Invitrogen). Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Both human and mouse hepatocytes were plated on collagen I coated tissue culture plate at a density of 1×105/cm2 in William’E medium (Thermo Fisher) and 10% FBS (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Compound solutions were then dispensed at 10nl/well directly into columns 1–22 of 384-well assay plates (Nunc 242757), while columns 23 and 24 were reserved for controls (see below) ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in double filtered sea water for 60 minutes on a shaking plate followed by three 1 minute 1X PBS Tween (1X Phosphate Buffered Saline: Gibco #70011-044 ...
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bioRxiv - Immunology 2023Quote: ... Between 1×106 and 1×107 splenocytes were transferred to 96-well plates for staining with freshly prepared LIVE/DEAD Aqua (Invitrogen) and TruStain FcX (anti-mouse ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: HepG2 cells and HepG2DNAJB1-PRKACA clones were transfected in 96-well plate with 0.25-100 nM Silencer small interfering RNA (siRNA) (Negative Control: #1, CDK7: s2830) (Life Technologies) using Lipofectamine RNAiMAX transfection reagent (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was extracted from the femur and tibia of dissected mice and plated on non-tissue culture-treated 15-cm Petri dishes at a density of 1 x 107 cells per plate in macrophage differentiation medium (DMEM, Gibco) supplemented with 2mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... into a single-cell suspension and seeded on Matrigel-coated plates with ROCK inhibitor and knockout serum replacement (1%, Gibco).
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bioRxiv - Physiology 2024Quote: ... Monocytes were cultured in 12-well plates at a density of 1 × 106/mL in Roswell Park Memorial Institute media 1640 (Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2024Quote: Bd isolate JEL 197 was grown in 1% tryptone broth or on 1% tryptone agar plates (Difco Laboratories, Detroit, MI) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco) at 19°C.
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bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated TACC3 Δ699-765 (50 µL of 10 µg mL−1, diluted in PBS) was immobilized on preblocked HBC Streptavidin plates (ThermoFisher) for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Microbiology 2024Quote: ... cells were seeded in a 24-well plate at a density of 105 cells per well in 1 ml MEM (Gibco) and grown for 5-6 days ...
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bioRxiv - Microbiology 2024Quote: ... Vero cells (1 × 105 cells per well) in a 24-well cluster plate were transfected with 1 μg plasmid DNA using the Lipofectamine 3000 system (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% sodium deoxycholate, 0.1% SDS), supplemented with phosphatase inhibitors (10 mM NaF, 20 mM β-glycerophosphate) and protease inhibitor (Thermo Scientific) at 107 cells/ml and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 40 ng of viral DNA and 1 µM reverse primer IRES-NADL-rev in 20 µl reaction with SuperScript IV reverse transcriptase (ThermoFisher, 18090010). 461 bp DNA fragment was amplified using IRES-NADL forward and reverse primers and Q5 Hot Start polymerase (NEB ...
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bioRxiv - Genomics 2020Quote: ... Then 107 293T cells were suspended and lysed for 20 mins with 1 ml RIPA lysis buffer (Thermo Fisher Scientific, USA) containing 1 μl of protease inhibitor (Beyotime ...
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bioRxiv - Developmental Biology 2021Quote: ... An appropriate fluorescently coupled secondary antibody was added for 1hr at RT followed by three washes with 0.1% PBST (first wash for 20 min and remaining for 10 min each) and mounted in Slow-Fade Gold (Molecular Probes).
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of the plasmid was digested with KpnI and BamHI in a 20 μL reaction for 1 h using FastDigest enzymes (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... 0.1% (w/v) Tween 20) and the proteins of interest detected with monoclonal mouse anti-Pgk1 (R & D Systems, Fisher Scientific) and monoclonal mouse anti-HA (12CA5 ...
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bioRxiv - Immunology 2019Quote: ... Electroporation was performed at 1600 V 10 ms 3 pulses settings for activated cells and at both 2200 V 20 ms 1 pulse and at the same settings as activated cells for unstimulated cells using Neon electroporation device (Thermo Fisher). For DNA electroporation experiments in activated cells ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed in 0.3% PBS-T 3 times for 20 minutes before being transferred to a solution containing Neurotrace 640/660 (Invitrogen, 1:800) prepared in 0.3% PBS-T for 2-3 hours at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Medium for H9 and PEN8E-derived organoids was supplemented with 20 ng/ml insulin-like growth factor 1 (IGF1; ThermoFisher Scientific) and 55 nM beta-mercaptoethanol (2-ME ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated at room temperature for 20 min with corresponding species-specific secondary antibodies (Alexa series at 1:2000, Life Technologies). The slides were covered with Vectashield medium containing 4′,6-diamidino-2-phenylindole (DAPI ...