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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Wells were washed with wash buffer (0.05% Tween 20 in PBS) and blocked with 2% BSA (Biowest) in PBS containing 1% Casein blocker (Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... except the induction temperature was 20 C and the soluble fraction was passed through a 1 mL column packed with amylose resin (Thermo Scientific). The purification buffer was the same as what we used for the GST-protein purification ...
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bioRxiv - Plant Biology 2020Quote: ... The amplicons were checked by electrophoresis and 25 µl of each pooled PCR product were purified and normalized using SequalPrep Normalization Plate Kit (Invitrogen GmbH, Karlsruhe, Germany). We then pooled the samples and the mock community and proceeded for a single library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing reactions were carried out on 96-well plates using BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems, foster City, CA) for 30 cycles ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from 10-day old seedlings grown on ½ MS plates under long-day conditions using PureLink RNA Mini Kit (Thermo Fisher Scientific, 12183025). One microgram of total RNA was reverse transcribed into cDNA with Protoscript II (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat. No. A1051001, ThermoFisher, Waltham, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was produced from mRNA from cells lysed directly in the culture plate using the TaqMan® Gene Expression Cells-to-CT™ Kit (ThermoFisher Scientific). qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 37°C for 24 hr and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: 10 μL of PCR product for each sample was normalized using a SequalPrep 96-well Normalization Plate Kit (Thermo Fisher Scientific, Waltham, MA). Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: DNA in 100 µl of undiluted cell-free BALF (first wash) was quantified in DNase-free 96-well plates (TPP®) using Quant-iTTM Pico GreenTM dsDNA Assay Kit (Thermo Fisher Scientific) following manufacturers’ instructions.
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bioRxiv - Zoology 2019Quote: Ten microliters of PCR amplicon was purified and normalized for concentration prior to library preparation using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, USA). DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Genomics 2022Quote: ... and split half for maintenance in a well of a 96-well plate and half used for genotyping using the Phire Animal Tissue Direct PCR Kit (Thermo Fisher Scientific, F140WH) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µL of each concentration was transferred into a U-shaped 96-well plate and incubated with 100 µL QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™) for 40 minutes along with the sEV samples to be detected on O-NEXOS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for final quality assurance the CFFT Lab subjected all its plasmids from the daughter plate to next generation sequencing (NGS): DNA from plasmid constructs was quantitated using Qubit dsDNA HS Assay Kit (Q33230, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9 µL of the dPCR reaction mix was added to each well of a QuantStudio Absolute Q MAP16 Plate Kit (PN A52865, ThermoFisher Scientific, Waltham, MA). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
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bioRxiv - Cell Biology 2024Quote: ... was used to measure collagen in lung lobes following kit instructions and optical density was measured using a Varioskan LUX plate reader (Thermo Fisher Scientific, VL0000D0).
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bioRxiv - Cancer Biology 2019Quote: ... Synthesis of the first strand of cDNA was performed from 1 μg of total cellular RNA in 20 μl volume using oligo(dT)18 primer and SuperScriptTM III Reverse Transcriptase Kit (Thermo Fisher Scientific, Carlsbad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 2 μg of RNA were reverse transcribed to cDNA in a 20 μl reaction using the High Capacity RNA-to-cDNA Master Mix kit (Applied Biosystems by Life Technologies, Carlsbad, USA), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg RNA was processed in a total reaction volume of 20 μl using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Waltham, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cytokines and chemokines analyzed in media of primary MC cultures by multiplex bead array analysis used a FlexMap3D platform (Luminex, Austin, TX) and Cytokine Mouse Magnetic 20-Plex Panel kit (Invitrogen/ThermoFisher Scientific, Waltham, MA). The FlexMap3D results were confirmed by individual commercially available enzyme-linked immunoassay (ELISA ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of total RNA was converted into cDNA in a 20 μl reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life technologies, Thermo Fisher Scientific) according to manufacturer's instructions and subsequently 1/5 diluted in sterile RNAse and DNAse free water ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of the cDNA first strand was performed from 1 μg of total RNA in 20 μl volume using oligo(dT)18 primer and SuperScriptTM III Reverse Transcriptase Kit (Thermo Fisher Scientific, Carlsbad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the resulting supernatant of each sample was transferred to a labeled microcentrifuge tube and stored at -20°C The Quant-iT dsDNA Assay Kit (Thermo Fisher Scientific, MA, USA) was used to measure DNA concentrations prior to PCR amplification ...
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bioRxiv - Plant Biology 2022Quote: 2 μg DNase treated RNA per 20 μl reaction were used to generate cDNA with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 250ng of total RNA was converted to cDNA in a 20 μL reverse transcription reaction using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA). The reaction involved ...
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bioRxiv - Genomics 2023Quote: ... 100 cells were sorted in 5 µL RT Mix (Superscript® VILO™ cDNA Synthesis Kit, 20 U/ µL SUPERase-In™ RNase Inhibitor, 10% NP-40 Detergent Surfact-Amps Solution, ThermoFisher Scientific) using a BD FACSAria™ Fusion and stored at −80°C.
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bioRxiv - Pathology 2023Quote: ... 1 μg of total RNA was reverse transcribed in a 20 μl reaction mix using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... One μg of RNA was used for a 20-µL first-strand cDNA synthesis reaction using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA) following the manufacturer’s guidelines ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Fluorescein-labeled dsRNA was synthesized using 2 µL of the plasmid pT777T-YFP in 20 µL reactions of the MEGAscriptTM RNAi Kit (Thermo Fisher, Waltham, MA, USA) with 2 µL of the Fluorescein labeling mix (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant containing the lysate was collected by centrifugation at 15,000 rpm for 20 min and quantified using Pierce™ BCA Protein Assay Kits (Thermo Fisher Sc., Cat#23227). Equal amounts of protein were mixed with SDS sample buffer and boiled at 95°C for 5 min ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: 1 μL of RNA was assayed using the TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher, #4392938). Specific transcripts were detected using Taqman assays ...
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bioRxiv - Biophysics 2020Quote: ... 30,000 cells were seeded into a 24-well plate (NuncMicroWell Plates with Nunclon; Thermo Fisher Scientific, Waltham, MA). Transfection was carried out at day 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ninety-six-deep well plates and 500 cm2 cell culture plates were purchased from Fisher Scientific (Loughborough, UK). Millipore 96-well GF/B filter plates were purchased from Receptor Technologies (Warwick ...
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bioRxiv - Microbiology 2020Quote: ... and vancomycin in Mueller-Hinton broth using sensititre plates EUVENC Sensititre® plates (Thermo Fisher Scientific, Waltham, USA) and home-made 96-well microtiter plates for levofloxacin following the CLSI guidelines (19).
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bioRxiv - Immunology 2021Quote: ... to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
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bioRxiv - Cell Biology 2023Quote: ... 800,000 cells were seeded into a 6-well plate (NuncMicroWell Plates with Nunclon; ThermoFisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Microbiology 2023Quote: ... cells and supernatants or of mGAM were distributed into 96-well plates (V-bottom storage plates, Thermo Scientific) according to drug-treatment concentration ...
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bioRxiv - Immunology 2024Quote: ... Ninety-six well polystyrene plates NUNC MaxiSorp™ or NUNC 384-Well Clear polystyrene plates (Thermo Fisher Scientific) were coated with 2,4-dinitrophenyl conjugated to BSA (DNP-BSA ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in lysis buffer (20 mM NaP pH 7.4, 50 mM NaCl and 20 mM imidazole) supplemented with protease inhibitor cocktail (Thermo Scientific) and lysed by sonication (10 cycles ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The dry membrane was washed in blocking buffer (PBS + 4% milk powder + 0.1% Tween-20) for 20-30 min and subsequently incubated for 45 min in Streptavidin-HRP (Thermo Scientific) diluted 1:3000 in blocking buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with 20 nM siRNA oligonucleotides and 20 nM negative control siRNA using Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were rinsed in 1X PBST (PBS+0.01% Tween-20) (20 mins/rinse, twice) and stained with 10nM DAPI (ThermoFisher Scientific) in 1X PBST overnight at 4°C ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... the samples were subjected to an additional treatment with 20 µL of 20 mg/mL RNaseA (Invitrogen PureLinkTM RNaseA, France). Finally ...