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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... with 20% knockout serum (Life Technologies, 10828010), Gluta-Max (1:100 Life Technologies 35050079) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % KO serum replacement (KOSR, Gibco #10828028), 2 mM GlutaMaxTM (Gibco #35050-038) ...
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bioRxiv - Bioengineering 2024Quote: ... 20% fetal bovine serum (FBS) (Gibco, Thermofisher), and 1% penicillin-streptomycin (PS ...
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bioRxiv - Bioengineering 2024Quote: ... 20% fetal bovine serum (FBS) (Gibco, Thermofisher), and 1% penicillin-streptomycin (PS ...
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bioRxiv - Cell Biology 2024Quote: ... in 0.05% Tween-20 (Fisher Scientific, UK) in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 mM HEPES (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2024Quote: ... 10 % FBS and 20 mM HEPES (Gibco). In some instances live cell dyes ...
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bioRxiv - Biochemistry 2024Quote: ... 20 U SUPERase·In RNase Inhibitor (Invitrogen, AM2696), 1 uM RT-1306 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/ml bFGF (Cat #PHG0261, Invitrogen), hydrocortisone and B27 (cat #12587010 ...
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bioRxiv - Microbiology 2024Quote: ... and RNAseI (20 U, ThermoFisher ref. EN0601). DNAse was then inactivated using EDTA at a final concentration of 20 mM ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 20% FBS (Gibco Cat# 10100139), 1% GlutaMAX (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 20% Superblock (Thermo Fisher) plus 10% goat or donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM Glutamine (Gibco, ThermoFisher Scientific, #25030), 100 ng/mL NGF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... 2.8% (v/v) 20% glucose (#A2494001, ThermoFisher), 7% Tryptose phosphate broth (#18050039 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 U/ml SUPERase•In (Invitrogen #AM2696), 1:200 Millipore protease inhibitor IV #539136) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 U/ml SUPERase•In (Invitrogen #AM2696), 1:200 Millipore protease inhibitor IV #539136) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 20% knockout serum replacement (Gibco), 1 mM L-glutamine (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM Glutamine (Gibco, ThermoFisher Scientific, #25030), 100 ng/mL NGF (PeproTech ...
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bioRxiv - Synthetic Biology 2024Quote: ... in 1X PBS-Tween 20 (Thermo Scientific). To detect produced zipFvs ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were sorted in a BD FACSAria™ III into 96-well plates loaded with RT-STA reaction mix (CellsDirect™ One-Step qRTPCR Kit, Invitrogen) and 0.2x specific TaqMan ® Assay mix (see Table 1 for assays list).
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bioRxiv - Neuroscience 2019Quote: ... that were either positive or negative for NIAD4 were sorted into 96 well plates (10 cells/well) containing 10 μl of Lysis Buffer from the Single Cell to Ct kit (Thermo Fisher Scientific), using the FACSAria™ III cell sorter ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were sorted in a BD FACSAria™ III directly into 96-well plates loaded with RT-STA Reaction mix (CellsDirect™ One-Step qRTPCR Kit, Invitrogen, according to the manufacturer’s procedures ...
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bioRxiv - Bioengineering 2021Quote: ... The 300 μl collected was aliquoted into an individual well in a 96-well microplate containing 100 μl each (3 wells total for a single well in the 24-well plate) and subjected to a CyQuant© Direct Proliferation Assay Kit (Thermo Fisher) per the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from Caco-2 cells grown in 24 well plates for 21 days using the PureLink RNA Mini kit (Thermo Fisher, 12183025) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... aliquots of SAGs were transferred to replica plates for DNA yield quantification using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific). For sequencing analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed in 96 well plates and RNA was extracted using Applied Biosystems MagMax 96 total RNA isolation kit (Thermo Fisher, # AM1830) with on bead DNase digestion ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Immunology 2020Quote: ... Each plate was pooled and the plate libraries were quantified on a Qubit Fluorometer 3.0 using a dsDNA High-sensitivity Kit (Life Technologies, Carlsbad, CA). The plates were then pooled in equal volumes and the pooled library was then diluted to 6 μM and sequenced using a 500-cycle V3 reagent on an Illumina MiSeq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sizes were determined using an Agilent 2200 TapeStation system (∼550bp) and libraries normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific). Library concentration was calculated using a SYBR green quantitative PCR (qPCR ...
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bioRxiv - Physiology 2023Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted in a 96-well format from the consolidated Prospector® culture plates using the Extract All Kit (Applied Biosystems). 20µL of culture was combined with 20µL of Lysis Solution and incubated for 10 minutes at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of lactate in the reaction mix was measured with the Lactate Assay Kit (colorimetric assay) by reading the absorbance using Multiskan SkyHigh plate reader (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Immunology 2021Quote: ... First strand cDNA was synthesized from 200 ng of total RNA and a mixture of Oligo (dT) and random hexamer primers in a 20 μl reaction volume using RevertAid first strand cDNA synthesis Kit (Thermofisher Scientific, Waltham, MA). PSurity of the cDNA was measured by A260/A280 ratio using UV spectroscopy (Thermofisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA and oligo primers were used for cDNA synthesis in a total reaction volume of 20 µL High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 4368814). qRT-PCR reactions were prepared using SYBR-Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA sample (40ng) was reverse-transcribed to cDNA using RevertAid First Strand cDNA Synthesis Kit with oligo (dT)20 primers (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 500 ng of RNA was reversed transcribed into cDNA in a 20 μl reaction volume using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Zebra mussel tissue RNA was extracted using the Qiagen RNeasy Plus Universal kit from tissues stored at −20°C in RNAlater™ (Ambion, Carlsbad, CA). RNA concentration was assessed using Nanodrop ...
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bioRxiv - Bioengineering 2019Quote: ... dead cells were stained for 20 minutes prior to fixation using the LIVE/DEAD™ Fixable Dead Cell Stain Kit (Thermo Fisher Scientific) at a concentration of 0.5 µl stain in 500 µl Hank’s Balanced Salt Solution per scaffold ...
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bioRxiv - Cell Biology 2020Quote: Cytokines and chemokines analyzed in media of primary MC cultures by multiplex bead array analysis used a FlexMap3D platform (Luminex, Austin, TX) and Cytokine Mouse Magnetic 20-Plex Panel kit (Invitrogen/ThermoFisher Scientific, Waltham, MA). The FlexMap3D results were confirmed by individual commercially available enzyme-linked immunoassay (ELISA ...
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bioRxiv - Developmental Biology 2020Quote: ... pooled from 20-24 jellyfish was extracted using the RNAqueous-Micro kit according to the manufacturer’s instructions (Ambion, Invitrogen, Thermo Fisher Scientific, #AM1931). Two biological replicates were generated for each sample type ...
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bioRxiv - Genomics 2022Quote: ... Each well was made up to 20 uL with H2O and bisulfite conversion was performed following manufacturer protocols using the MethylCode BC conversion kit (Applied Biosystems, Cat. MECOV50) and eluted twice (12.5 uL each using elution buffer ...
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bioRxiv - Genomics 2022Quote: ... The final library was eluted with 13 µl elution buffer (EB) for 20 min at 48°C and DNA concentration was quantified using the Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Q32850). A total of 400 ng was carefully mixed with 37,5 µl sequencing buffer (SQB) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared with 2 μg of the treated RNA in 20 μL total volume of reaction mix using High-capacity cDNA reverse transcription kit from Applied Biosystems (REF 4368814). The cDNA was further diluted by 1:10 to obtain a final concentration of 10 ng/μL ...
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bioRxiv - Genetics 2022Quote: ... centrifuged at 10000g for 20 min at 4°C and protein levels measured from the supernatant using BCA kit (Thermo Fisher Scientific, UK). 60μg of protein lysate was incubated with 3.6mM ASA in final volume of 50μl ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from 20 mg seeds (dry weight) using the RNAqueous™ Small scale phenol-free total RNA isolation kit (Ambion: catalog #1912) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The first-round PCR product was purified and eluted into 20 µL of ddH2O using GeneJET Gel Extraction Kit (Thermo Fisher Scientific #K0691). The second-round PCR was then reamplified in 20 µL volume ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min or with a mix of SYTO 9 (3.34 µM) and propidium iodide (PI, 20 µM) from the LIVE/DEAD BacLight Bacterial Viability kit (#L7012, Molecular Probes, Thermo Fisher Scientific) for 15 min ...