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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: CD3/CD28 Dynabeads were removed from stimulated primary human CD4+ T cells using a DynaMag-2 magnet (Invitrogen). For antibody staining ...
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bioRxiv - Biochemistry 2020Quote: The eEF2K-KO CRISPR targeting vector was the GeneArt CD4 CRISPR Nuclease Vector (ThermoFisher Scientific, Scoresby, VIC, Australia). The guide ssDNA sequence was 50-AGTGAGCGGTATAGCTCCAG-30 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by negative depletion using magnetic streptavidin beads (MagniSort SAV Negative Selection beads; Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8 and CD4 T cells were activated and expanded with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from purified CD4 T cells by Trizol-Column (PureLink RNA Mini kit; Thermo Fisher Scientific). RNA was quantified by U.V ...
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bioRxiv - Microbiology 2021Quote: ... CD4(+) T cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; MiliporeSigma) in complete RPMI-1640 (Thermo Fisher) supplemented with 10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... In brief, effector T cells (Teff, CD4+CD25-) were stained with CellTrace Violet (CTV, ThermoFisher Scientific, 2.5 µM) and cultured with Tregs (CD4+CD25+ ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from CD4+ splenic T-cell populations of interest using Dynabeads mRNA purification kit (ThermoFisher Scientific). cDNA was synthesised from each RNA preparation by adding the following to each sample ...
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bioRxiv - Immunology 2021Quote: ... The following day the cells were stained with antibodies against extracellular markers (MHC-II eFluor450, eBioscience 48-5321-82; CD4 BV785, bioLegend 100552; NK1.1 PerCP-Cy5.5, BD Biosciences 561111; CD11b PE, Invitrogen 12-0112-82 ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; MiliporeSigma) in complete RPMI-1640 (Thermo Fisher) supplemented with 10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD25-CD62Lhi naïve T cells from CD45.1 mice were labeled with 1 μM CFSE (Thermo Fisher Scientific), and the labeled T cells were used as responder T cells ...
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bioRxiv - Immunology 2023Quote: ... HIV-1JRFL or HIV-1AD8 infected primary CD4+ T cells were stained with viability (AquaVivid; Thermo Fisher Scientific) and cellular (cell proliferation dye eFluor670 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2023Quote: ... Isolated CD4+ T cells were cultured in complete RPMI-IL2 media (RPMI-1640 media (cat# 11875093, Thermofisher Scientific) supplemented with 10% heat-inactivated Cosmic Calf Serum (cat#SH30087.03 ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated from CD4 T cells using the PicoPure RNA extraction kit (Cat# KIT0204, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells previously labeled with CMAC cell tracer (7-amino-4-chloromethylcoumarin, CellTracker Blue CMAC Dye, Invitrogen) were added to the microscope (Nikon ECLIPSE Ti-TimeLapse microscopy) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified and genomic DNA was extracted according to the “PureLink DNA mini kit” (Invitrogen). DNA concentration was acquired at the NanoDrop spectrophotometer (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... the responder cells (CD4+ T effectors and CD8+ T cells) were stained with cell trace violet (CTV, ThermoFisher) at 7 million/ml concentration and CTV diluted at 1:8000 in PBS ...
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bioRxiv - Immunology 2024Quote: ... and PB (live singlet CD4− CD14− CD19+ IgDlo CD20lo CD38+ CXCR5lo CD71+) were sorted using a Bigfoot (Invitrogen) into Buffer RLT Plus (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... CD62Lhi) T cells were purified (>95% purity) by negative selection using the Dynal Mouse CD4 Negative Isolation kit (Invitrogen). When indicated ...
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bioRxiv - Immunology 2021Quote: ... were sorted as described and CD5lo and CD5hi naïve CD4+ T cells were either directly added to 500μL TRIzol (ThermoFisher Scientific) or rested in complete RPMI supplemented with IL-7 for 22 hrs first ...
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bioRxiv - Genomics 2020Quote: ... CD4+ T cell activation was performed using magnetic beads coupled with CD3/CD28 antibodies (Thermo Fisher Scientific, Cat: 11452D) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Immunology 2022Quote: ... cells at 30 h (CD8+T) or 48 h (CD4+ T) after stimulation were permeabilized using Cytofix/Cytoperm (Invitrogen) and stained with a mixture of anti-Ki-67 antibody and NucSpot Far-Red for 15 min at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... Isolated T cells (including CD4+ and CD8+) were CFSE labeled by incubating them with 80 μm CSFE (Thermo Fisher) in CCM for 5 min at RT as described previously58 ...
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bioRxiv - Cell Biology 2021Quote: ... and immunolabeled with 10 μg/ ml anti-CD4 (Q4120) for 1.5 hr at RT followed by detection with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Finally ...
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bioRxiv - Immunology 2021Quote: ... For proliferation assay memory CD4+ T cells were stained with CellTrace™ Violet Cell Proliferation Kit (Thermo Fisher Scientific) according to the Manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: Sex and age-matched CD4+ T cells were enriched from CD45.1 and naïve control or RorcCre Tet1/3 mice using the Dynabeads untouched CD4 T cell kit (ThermoFisher Scientific), ensuring >85% purity ...
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bioRxiv - Immunology 2021Quote: ... FACS-sorted CD4+CD8−CD25−CD62L+CD44lo naïve T cells were seeded in T cell medium [RPMI 1640 (Gibco), 10% heat-inactivated FBS (Atlantis) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells of PLWH were sorted based on LIVE/DEAD Cell Viability staining (Thermo Fisher, Waltham, MA, USA) using a LSR Aria cell sorter and the FACSDiva software (Becton Dickinson ...
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bioRxiv - Immunology 2021Quote: Human CD4 T cells were MACS-sorted from PBMC and subsequently pre-activated with α-CD3/CD28 Dynabeads (Thermofisher) at a bead-to-cell ratio of 1:1 for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then labeled with the CellTrace™ Violet Cell Proliferation Kit (Invitrogen™, Catalog No. C34557) before co-culturing at 37ºC with antigen-pulsed dendritic cells at a ratio of 1:10 ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells were combined 1:1 and stimulated with CTS™ Dynabeads™ CD3/CD28 (Invitrogen) at a 1:1 ratio overnight in X Vivo-15 (Lonza ...
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bioRxiv - Immunology 2023Quote: ... and plated in a 1:1 ratio with Itgb8+ or Itgb8+ CD4+ TCm in RPMI containing 0.88 mM L-Glutamine (Gibco), 0.88 mM sodium pyruvate (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were labeled for 10 min at 37°C with CellTrace™ CTV (Molecular Probes, ThermoFischer Scientific) and then activated with plate-bound anti-CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: CCR6+CCR2+CD4+ and total unselected memory T cells were stimulated with cell stimulation cocktail (00-4970-03; Invitrogen) for 3 h at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ CD44loCD62Lhi CD25− naive T cells were isolated from the spleen of CD45.1+ mice by cell sorting after positive enrichment for CD4+ cells using MACS LS columns (Miltenyi) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher). CD4+YFP+ Treg cells were isolated from the spleen of Foxp3cre or PHD2ΔTreg mice by cell sorting ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 million CD8+ or CD4+ T cells were stained with 0,07 uM CellTrace Far Red Cell Proliferation dye (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... near-full-length proviral amplification was performed on DNA extracted from CD4+ T cells by nested PCR using Platinum Taq DNA Polymerase High Fidelity (Invitrogen) such that ∼25% of the resulting PCR reactions yielded an amplicon ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 mL kit (ThermoFisher). Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Cell Biology 2021Quote: ... expressing CD4-SIV Env chimeras were seeded and transfected the same day with siRNA oligonucleotides (145 nM) using Oligofectamine (Invitrogen). Oligonucleotides targeting the μ2 subunit of adaptor protein complex 2 (AP2 ...
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bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...
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bioRxiv - Genomics 2021Quote: ... we stimulated these CD4+ T cells with CD3/CD28 crosslinking for 45 hours (Gibco, Dynabeads Human T-Activator CD3/CD28). We then performed ChIP-seq (2 million stimulated CD4+ T cells) ...
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bioRxiv - Immunology 2022Quote: ... and total GC B cells (live singlet CD4− CD19+ IgDlo CD20+ CD38int CXCR5+ CD71+) and LNPC (live singlet CD4− CD19+ IgDlo CD20lo CD38+ CXCR5lo CD71+) were sorted using a Bigfoot (Invitrogen) into Buffer RLT Plus (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were pre-enriched by magnetic negative depletion using streptavidin beads (MagniSort SAV Negative Selection beads, Thermo Scientific) according to the manufacturer’s description ...