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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... purified CD4+FYP+ Treg cells (1 × 105) were cultured with Dynabeads mouse CD3/CD28 T cell stimulator (25 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Immunology 2023Quote: ... purified CD4+FYP+ Treg cells (1 × 105) were cultured with Dynabeads mouse CD3/CD28 T cell stimulator (25 μl/ml) (Thermo Fisher Scientific) and IL-2 (1500 U/ml ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells (1 × 105) were cultured with Dynabeads mouse CD3/CD28 T cell stimulator (25 μl/ml) (Thermo Fisher Scientific) and IL-2 (1500 U/ml ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+FYP+ Treg cells (1 × 105) were cultured with Dynabeads mouse CD3/CD28 T cell stimulator (25 μl/ml) (Thermo Fisher Scientific) and IL-2 (1500 U/ml ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were labelled with a LIVE/DEAD® Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific, Waltham, MA, USA) to distinguish dead cells in a 15 ml centrifuge tube at 4 °C for 30 min and then stained with BUV737 mouse anti-human CD4 (SK3) ...
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bioRxiv - Cancer Biology 2024Quote: Primary T-cells (CD4 and CD8; 1:1 ratio) were activated for 24 hours with Dynabeads™ Human T-Expander CD3/CD28 (Thermo Fisher) at 3:1 bead ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from the spleens of 1C6 mice using mouse CD4 MicroBeads (Miltenyi) and labeled with CellTrace Violet (CTV; Thermo Fisher Scientific). CTV-labeled 1C6 CD4+ T cells were cultured with B cells at a ratio of 1 CD4 ...
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bioRxiv - Microbiology 2024Quote: ... The percentage of infected cells (p24+, CD4-) was determined by gating on the living cell population according to viability dye staining (Aqua Vivid; Thermo Fisher Scientific). The percentage of ADCC was calculated with the following formula ...
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bioRxiv - Microbiology 2024Quote: ... The percentage of productively infected cells (p24+, CD4-) was determined by gating on the living cell population according to viability dye staining (Aqua Vivid; Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... The HIV-1-infected primary CD4+ T cells were stained with AquaVivid viability dye and cell proliferation dye eFluor670 (Thermo Fisher Scientific) and used as target cells ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were purified from spleens and LNs of B6-SJL-ptprca donor mice using the Dynabead® FlowComp™ Mouse CD4+ Enrichment kit (Invitrogen) and labelled with CellTrace Violet (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The percentage of productively infected cells (p24+) was determined by gating on the p24+/CD4-living cell population using a viability dye staining (Aqua Vivid, Thermo Fisher Scientific). Samples were acquired on Fortessa cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... specific for I-Ab -restricted CD4 T cell epitopes 33 were maintained in complete RPMI (cRPMI) which consists of RPMI 1640 (Thermo Fisher Scientifics) supplemented with 10 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... T cells were cultured for 4hrs at 37°C then stained for surface marker CD4 (FITC) (Life Technologies, Cat 11-0049-42) prior to fixation and permeabilisation for intracellular staining of anti-human IFN-γ (Th1 ...
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bioRxiv - Cell Biology 2024Quote: ... Enriched cKit+ cells were then incubated with an anti-Lineage antibody cocktail (including biotinylated Gr1[LY-6G/LY-6C], CD11b, CD4, CD8a, CD45R[B220], IL7-R, TER119 (all from Thermo Fisher Scientific)) for an additional 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Donor specific CD4+ T cells were plated at 1×106 cells/ml with CD3/CD28 activation beads (Dynabeads, ThermoFisher Scientific, Loughborough, UK; #11132D) at a 1:1 ratio with 50 U/ml IL-2 at 2 ml per well in a 24 well plate and expanded for three days at 37°C in a CO2 incubator ...
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bioRxiv - Neuroscience 2021Quote: ... For GAL4CIII::tdTomato we utilized Gateway cloning to combine the R83B04 (CIII) enhancer with CD4-tdTomato by LR reaction (Invitrogen ref: 12538-120). Transgenic flies carrying a 2nd chromosomal insertion of CIII:tdTomato (docking site ...
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: The proliferation of CD4+CD25- effector T cells was measured by CFSE (CellTrace™ CFSE Cell Proliferation Kit, Thermal Fisher Scientific, Waltham, MA) dilution assay ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2020Quote: ... isolated SI CD8α+ IET (EpCAM-CD4-CD19-TER119- CD8α+) from wild type mice were labeled with CellTrace™ Violet (CTV, Thermo Fisher Scientific), and then labeled cells were incubated in the presence of recombinant IL-15/15Rα (50ng/mL ...
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bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from spleens and lymph nodes of donor C57BL/6 and IFNγ-/- mice using the MagniSort CD4+ T cell enrichment kit (ThermoFisher, #8804-6821-74). Flow cytometry confirmed that >85% of isolated live CD4+ T cells were naïve (CD44loCD62Lhi) ...
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bioRxiv - Immunology 2023Quote: ... We isolated CD4 T cells from pooled spleen and lymph nodes of 6-8-week-old Foxp3IRES-GFP mice using magnetic negative selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D), resting cells for at least 30 min at 4°C prior to electroporation ...
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bioRxiv - Genomics 2024Quote: CD4+ and CD8+ T cells were extracted from the lymphocyte fraction using the Dynabeads CD4 Positive Isolation Kit and Dynabeads CD8 Positive Isolation Kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were isolated from single cell splenocyte suspensions through the use of a mouse-CD4+ magnisort negative selection kit (Invitrogen, 8804-6821-74) as per the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated by negative magnetic sorting using a Dynabeads™ Untouched™ Mouse CD4 or CD8 Cells kit (Invitrogen™ #11415D #11417D) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: HKDC1-deficient cell lines were generated by CRISPR/Cas9 technique with plasmids that were assembled using the GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions with the following primers ...
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bioRxiv - Immunology 2021Quote: Tregs from EWAT were isolated from SVF cells using Dynabeads™ FlowComp™ Mouse CD4+CD25+ Treg Cells Kit (Invitrogen, Thermo Fisher Scientific, USA). T effector cells (Teff) ...
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bioRxiv - Immunology 2021Quote: Tregs from EWAT were isolated from SVF cells using Dynabeads™ FlowComp™ Mouse CD4+CD25+ Treg Cells Kit (Invitrogen, Thermo Fisher Scientific, USA). T effector cells (Teff) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The isogeneic control line (WtLine#1) was generated from CFLine#1 using the GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (Thermo Fisher, cat # A21175); The genetic correction of F508del in CFTR gene was verified by CCRM using PCR and Sanger sequencing at the targeted locus ...
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bioRxiv - Immunology 2022Quote: We isolated T cells from PBMC of patients and healthy controls for autologous mixed lymphocyte reaction (MLR) assay using Dynabeads Untouched Human CD4+ T Cells kit (Invitrogen Dynal cat. No.113.52D, US). For MLR ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were incubated in the presence of an anti CD16/32 Fc receptor-blocking antibody followed by surface staining of the following antibodies in three panels: Panel 1: APC-Cy7 CD3, FITC CD4, PerCP-Cy5.5 CD8, APC CD25 (BD 557596, 551162, 565159) (ThermoFisher 11-0042-86, 25-0441-82, CD25); Panel 2 ...
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bioRxiv - Immunology 2024Quote: ... The effector CD4+ T cells (1.0×107/mL) were stimulated with Dynabeads™ Mouse T-Activator CD3/CD28 (Thermo fisher scientific, at the ratio=1:1) in 24 well plate at 37°C for 72 h ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% Anti-mycotic Anti-biotic (Anti-Anti) (Gibco, Waltham, MA) at 5% CO2 and 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... (anti-Claudin-1, anti-Claudin-5, anti-Occludin, anti-ZO-1; Invitrogen), in a 1:100 dilution in 10% donkey serum in PBS with 0.1% Saponin and incubated overnight on the Brain-Chip at 4°C ...
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bioRxiv - Genomics 2022Quote: ... + 1x Anti-Anti (Gibco)) ...
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bioRxiv - Biophysics 2022Quote: ... and Anti-Anti (Gibco). P2 virus was added to cells ...
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bioRxiv - Neuroscience 2021Quote: ... and Anti-Anti (ThermoFisher) and kept at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS.
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bioRxiv - Biophysics 2023Quote: ... and Anti-Anti (Gibco). P2 virus was added to cells ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Anti-Anti (Invitrogen); 0.2% β-mercaptoethanol (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% Anti-Anti (Gibco), 13 mM d-glucose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% anti-anti (Gibco), and 5% PFHM-II (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-anti (Invitrogen, containing 100 μg/mL streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... + 1% Anti-Anti (Gibco)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-anti (Thermo Fisher) in 5% CO2 at 37 °C until 80% confluency ...
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bioRxiv - Biophysics 2024Quote: ... 0.5X Anti-Anti (Gibco), 20 mM Sodium Lactate (Sigma-Aldrich) ...