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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD4 T cells were purified by negative selection with magnetic beads as described above and cultured in Advanced RPMI (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... CD3-CD19+CD27- B cells and CD4+CD19-CD45RAloCXCR5+CD25hiCD127lo Tfr cells were sorted and resuspended in RPMI 1640 medium (GIBCO) with 10% FBS (Hyclone) ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
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bioRxiv - Immunology 2024Quote: ... The purified CD4+ T cells were stained with Far-Red using CellTrace Cell Proliferation Kit (Thermo Fisher Scientific, Waltham, MA) before coculture with CD11c+ DCs ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Immunology 2023Quote: Purified CD4+ naïve T (Tresp) cells from CD45.1+ C57BL/6 mice were stained with CellTrace violet (CTV) (Thermo Fisher Scientific). Tresp cells (5 × 104 ...
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bioRxiv - Genetics 2023Quote: ... near full-length HIV proviral amplification was performed on genomic DNA extracted from CD4+ T-cells using Platinum Taq DNA Polymerase High Fidelity (Invitrogen), where IPDA-determined total proviral loads were used to dilute DNA such that no more than 30% of resulting nested PCR reactions yielded an amplicon (protocol described in 40) ...
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bioRxiv - Biophysics 2023Quote: CV1 cells were transfected with mScarlet-CD4-PhoCl-RER and rtTA3 plasmids using the Neon Transfection System (Thermo Fisher Scientific) 24 hours before the experiment ...
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bioRxiv - Developmental Biology 2024Quote: ... Thymocyte suspensions were labeled with biotinylated CD4 and CD8 antibodies incubated with MACS Streptavidin Microbeads (Miltenyi, Biotec) in HBH buffer (HBSS (Gibco), 0.5% BSA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were isolated from mouse spleens using EasySep mouse naïve CD4+ T cell isolation kit (StemCell) and labeled with CellTrace Violet (CTV; ThermoFisher Scientific). CD11b+GR1+ cells were isolated from alum-treated or saline-treated animals and cultured with naïve CD4 T cells in the presence of 1μg/mL of OVAp 323-339 (InvivoGen ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+CD25+ Tregs from Apoe−/− or Ccr4−/−Apoe−/− mice were cocultured with carboxyfluorescein diacetate succinimidyl ester (Thermo Fisher Scientific)-labeled CD4+CD25− conventional T cells (2.5×104 cells ...
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bioRxiv - Immunology 2024Quote: ... and total GC B cells (live singlet CD4− CD19+ IgDlo CD20+ CD38int CXCR5+ CD71+) and LNPCs (live singlet CD4− CD19+ IgDlo CD20lo CD38+ CXCR5lo CD71+) were sorted using a Bigfoot (Invitrogen) into Buffer RLT Plus (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... Purified naïve DO11.10 CD4+ T cells as described above were stimulated using Dynabeads Mouse T-Activator CD3/CD28 (Gibco, catalog# 11452D) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: Freshly isolated CD4+ T cells were activated for 3 days on CD3/CD28 activation beads (Dynabeads ThermoFisher Scientific, Loughborough, UK; #11132D) as before ...
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bioRxiv - Bioengineering 2020Quote: FACS-purified CD3-CAR+ or CD3-CAR- CD4+ T cells (8 x 106 each) were lysed in Pierce™ IP Lysis Buffer (ThermoFisher) supplemented with Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2021Quote: ... HIV-1-infected primary CD4+ T cells were stained with AquaVivid viability dye and cell proliferation dye eFluor670 (Thermo Fisher Scientific) and used as target cells ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with unconjugated lineage rat antibodies (CD3, CD4, CD5, CD8, B220, Gr1, Mac1, and Ter119) followed by goat-α-rat PE-Cy5 (Invitrogen). Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cell proliferation was analyzed 96 hours post polyclonal activation in CD4+ T cells previously stained with cell trace violet dye (Thermo Fisher).
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bioRxiv - Cell Biology 2022Quote: ... were isolated by negative selection from whole blood of healthy donors (National Blood Service, Bristol, UK) using the Dynal CD4 negative isolation kit (Thermo Fisher). Cells were routinely maintained in culture overnight in RPMI-1640 ...
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... 1 million CD8+ or CD4+ T cells were stained with 0,07 µM CellTrace Far Red Cell Proliferation dye (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cell populations were isolated using a negative depletion method following the manufacturers instructions (Dynabeads CD4+ T cells, 11346D and Dynabeads CD8+ T cells, 11348D, Thermofisher UK). Following the isolation ...
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bioRxiv - Microbiology 2022Quote: ... CD4 T cell DNA was extracted using the QIAamp DNA Mini Kit and quantified on a NanoDrop 1000 (Thermo Fisher Scientific). IPDA was performed as originally described (45) ...
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bioRxiv - Immunology 2024Quote: ... These cells were cultured with purified OVA-specific CD4+ or CD8+ T cells that were stained with a cell proliferation dye (Cell Trace Violet, ThermoFisher Scientific) for three days (100,000 cells/well) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CD8-depleted PBMC fraction using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher). T cell purity was routinely checked by flow cytometry (>80%) ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of surface molecules of T cells were determined by labeling T cells with fluorochrome conjugated monoclonal antibodies: CD4-AlexaFlour532 (Thermo Fisher), CD3-Pacific Blue ...
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bioRxiv - Genomics 2023Quote: ... Primary CD4+ T cell vials were thawed at the time of the experiments and cells cultured in RPMI 1640 (Gibco, 11340892) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Physiology 2023Quote: ... The freshly isolated CD4+ T cells (1x 106/ml) were suspended in RPMI 1640 glucose-free medium (Gibco, Fisher Scientific, Sweden) supplemented with 2 mM glutamine ...
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bioRxiv - Physiology 2023Quote: ... The freshly isolated CD4+ T cells (1x 106/ml) were suspended in RPMI 1640 glucose-free medium (Gibco, Fisher Scientific, Sweden) supplemented with 2 mM glutamine ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1-infected primary CD4+ T cells were stained with AquaVivid viability dye and cell proliferation dye eFluor670 (Thermo Fisher Scientific) and used as target cells ...
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bioRxiv - Microbiology 2024Quote: ... CD4 T cell DNA was extracted using the QIAamp DNA Mini Kit and quantified on a NanoDrop 1000 (Thermo Fisher Scientific). IPDA was performed as originally described (46) ...
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bioRxiv - Immunology 2024Quote: CD4 T cells were sorted then stained with CellTrace™ Violet Proliferation Dye as per manufacturer protocols (Invitrogen™ Cat#:C34557). Labeled CD4 T cells were activated with anti-CD3/CD28 Dynabeads and cultured for four days before being analyzed by flow cytometry.
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CBMCs fraction in MACs buffer using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CBMCs fraction in MACS buffer using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: Fabs were isolated from sera of CD4 T cell depleted and IgG control mice using the Pierce Fab Micro Preparation Kit (Thermo Fisher) 30.
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bioRxiv - Immunology 2024Quote: ... enriched CD4+ T cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (1:16 bead:cell ratio; Thermo Fisher Scientific) overnight ...
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bioRxiv - Immunology 2024Quote: ... Primary mouse CD4+ and CD8+ T cells (1 × 106 cells/mL) were activated with Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher) at 4:3 cell-to-bead ratio for 2 days.
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bioRxiv - Biochemistry 2020Quote: HeLa cells expressing CD4 (TZM-bl, obtained from Dr. John Kappes via the NIH AIDS Reagent Program) were transfected using Lipofectamine2000 (Thermo Fisher Scientific) with pCG-GFP (a gift from Dr ...
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bioRxiv - Microbiology 2021Quote: ... Staining was done in a 1% goat serum and PBS solution containing 1:100 APC conjugate CD4 Stain (Life Technologies REF# MHCD0405) at 4°C for 1 hour in the dark ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleen and mesenteric lymph nodes single cells suspensions by positive separation using a Dynabeads FlowComp Mouse CD4 Kit (11461D, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Total numbers of CD4 T cells were calculated from unstimulated cultures by flow cytometry using CountBright™ absolute counting beads (Thermo Fisher). IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Jurkat T cells and their CD4 KO variant were transiently transfected with plasmid DNA using Neon® transfection system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes from 3- to 4-week-old wild type mice were stained with biotinylated antibodies for lineage markers (excluding CD4) and depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific). Flt3+ or Flt3-ETP were pre-sorted for yield and then 200 cells/well were sort-purified directly onto the OP9-DLL4 culture plate ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... The assay was carried out in a round bottom 96 well plate where 25,000 of conv CD4+ cells previously stained with cell trace violet (CTV, Thermo Fisher Scientific, USA) were co-cultured with Treg cells at Treg ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were labelled with a LIVE/DEAD® Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific, Waltham, MA, USA) to distinguish dead cells in a 15 ml centrifuge tube at 4 °C for 30 min and then stained with BUV737 mouse anti-human CD4 (SK3) ...
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bioRxiv - Cancer Biology 2024Quote: Primary T-cells (CD4 and CD8; 1:1 ratio) were activated for 24 hours with Dynabeads™ Human T-Expander CD3/CD28 (Thermo Fisher) at 3:1 bead ...