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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... human CD4+ T cells and splenocytes were labeled with CFSE (10μM; ThermoFisher, Waltham, MA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Murine CD4+ T cells were cultured in RPMI10% supplemented with 1 mM sodium pyruvate (Gibco), 1X nonessential amino acids (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells were cultured in RPMI10% medium (RPM1 1640 medium with Glutamax (Gibco) supplemented with 10% heat-inactivated FBS and 100 U/ml penicillin-streptomycin (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2024Quote: ... stained with CD4-Vio770 antibody (Miltenyi) and a Live/Dead (LVD) fixable violet dye (Invitrogen), fixed with 4% paraformaldehyde (ChemCruz ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4 T cells were labeled with CellTrace Violet following the manufacturer’s protocol (Life Technologies). Co-cultures of BMDCs and CD4 T cells were conducted with a 1:2 ratio ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Positive clones were purified using the Dynabeads® CD4 Positive Isolation Kit (Thermo Fisher Scientific) and thorough washing until cells directly bound to beads remained and no secondary bindings were present as monitored by stereomicroscopic evaluation ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... CD4+ T-cells were further cultivated and activated for 4 days in RPMI Glutamax medium (Gibco) containing 10 % heat-inactivated FCS (Sigma Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... and α-CD4–PE-Cy5.5 (clone S3.5) and α-IFNγ–AF700 (clone B27) from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CD4+ and CD8+ T cells were distinguished with live/dead viability stain (Thermo Fisher Scientific), followed by human CD3 and CD8 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Enriched CD4+ cells (>90% purity) were labelled with 5μM CellTrace Violet (Life Technologies, Carlsbad, CA, USA) and 5000 - 5 x 105 cells/recipient were transferred intravenously (i.v. ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+T cells were pre-labelled with 5mM CellTrace Violet division tracking dye (Thermo Fisher) according to manufacturer’s instructions and seeded into 96-well polystyrene plates at 80,000 cells per well in RPMI media with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ lymph nodes T cells were labeled with 2 µM CTV (Thermo Fisher Scientific) for 15 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Human primary CD4+ T cells were cultured overnight in RPMI-10 media (RPMI 1640, 11875119; Gibco), penicillin-streptomycin (100 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1JR-FL-infected primary CD4+ T cells were stained with viability (AquaVivid; Thermo Fisher Scientific) and cellular (cell proliferation dye eFluor670 ...
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bioRxiv - Immunology 2023Quote: ... followed by surface and intracellular antibody staining for CD4 (catalog 45-0042-82; Thermo Fisher Scientific); Ki67 (catalog 51-5698-82 ...
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bioRxiv - Immunology 2024Quote: Human CD4+ T cell proliferation was determined by the Click-it EdU detection assay (Thermo Fisher) [60] ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were labeled with CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific) and cultured with different ratios of Treg cells and anti-CD90.2 (30-H12 ...
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bioRxiv - Immunology 2023Quote: ... Cells were passed through 70µm meshes and incubated with CD4 and CD8 labeled Dynabeads (Thermofisher, 11031) to deplete most DP and SP thymocytes ...
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bioRxiv - Immunology 2024Quote: ... for 3 h then stained for CD4 and a LIVE/DEAD Fixable Dead Viability Dye (Invitrogen). Cells were then fixed and permeabilized (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated via positive magnetic selection according to the manufacturer’s instructions (Invitrogen 11331D). Cells were polarized with irradiated CD4-depleted feeders (1:10 ...
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bioRxiv - Immunology 2022Quote: ... NAD or ATP-treated CD4 cells (as described above) were resuspended in annexin-binding buffer (ThermoFisher) and incubated with 1µl 100µg/ml Propidium Iodide (PI ...
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bioRxiv - Immunology 2024Quote: ... T lymphocytes (both CD4 and CD8) isolated with Dynabeads flowcomp mouse pan T (Thermofisher, catalog: 11465D) from lymph nodes were first activated as Th0 with anti-CD3 and anti-CD28 plus IL-2 as indicated for CD4 cells above ...
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bioRxiv - Immunology 2024Quote: ... Enriched CD4+ T cells from Ocabfl/fl donors were labeled with QTracker 655 (Cat#Q25021MP, ThermoFisher) and enriched CD4+ T cells from Ocabfl/fl;CD4-Cre donors were labeled with QTracker 525 (Cat#Q25041MP ...
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bioRxiv - Immunology 2024Quote: ... CD4+T Cells were stimulated with Dynabeads Human CD3/CD28 T-Activator (Thermo Fisher Scientific, MA) for 30 minutes and 2 hours ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells underwent intracellular staining using the Foxp3 / Transcription Factor Staining Buffer Set (eBioscience Thermofisher) with anti-FOXP3-PE (Biolegend) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from total CD4+ T cells in vitro activated and differentiated using TRIzol (Invitrogen), followed by cDNA synthesis by RT-PCR ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were subjected to RNA extraction using the mirVana kit following the manufacturer’s instructions (Ambion). Reverse transcription of RNA to cDNA was performed with SuperScriptIII (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: Proliferation of purified CD4+ T cells was assessed by labeling cells with 2 μM CellTrace Violet (Invitrogen) or 0.5 µM CFSE (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Selected CD4+ T cells were activated by Dynabeads™ Human T-Activator CD3/CD28 kit (ThermoFisher Scientific) and were maintained in RPMI 1640 with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Sorted memory CD4+ T cells were labelled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher) and cultured at a ratio of 2:1 with irradiated autologous monocytes untreated or pulsed for 3 h with recombinant SARS-CoV-2 Spike protein (2.5 μg/ml) ...
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bioRxiv - Immunology 2020Quote: ... Purified CD4+ T cells were counted and labelled with 2 μM Cell Tracker Green CMFDA dye (ThermoFisher) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: Sorted naïve CD4 T cells were stained with 1μl/ml CellTrace Violet Proliferation Kit (Thermofisher, Cat. C34571) in the dark for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Lung CD4+ T cells were stimulated ex vivo with cell stimulation cocktail containing protein transport inhibitors (ThermoFisher) for 4 h at 37° C prior to staining ...
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bioRxiv - Immunology 2023Quote: ... or CD5hi naive CD4+ T cells was extracted using RNAqueousTM-Micro Total RNA Isolation Kit (Life Technologies) and cDNA converted using High-Capacity cDNA Reverse Transcription Kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... and HT2-CD4-17b were collected on a 300 keV Titan Krios (Thermo Fisher Scientific, Waltham, MA) cryo-electron microscope equipped with a K3 direct electron detector camera (Gatan ...
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bioRxiv - Immunology 2023Quote: Proliferation of stimulated CD4+ T cells was assessed by labeling cells with 2 μM CellTrace Violet (Invitrogen) prior to culture ...
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bioRxiv - Immunology 2023Quote: Overnight-rested mouse naïve CD4+ T cells constitutively expressing LifeAct-eGFP were labeled with CellMask Orange (ThermoFisher) to stain the plasma membrane following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and PB (live singlet CD4− CD19+ IgDlo CD20lo CD38+ CXCR5lo CD71+) were sorted using a Bigfoot (Invitrogen) into PBS supplemented with 0.05% BSA and immediately processed for single cell RNAseq ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells after HSP or TCR culture were incubated with LIVE/DEAD® Fixable Aqua (ThermoFisher) and human Fc blocker (BD Bioscience) ...
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bioRxiv - Genomics 2023Quote: ... Viable CD4+ T cells were identified for analysis using eBioscience Fixable Viability Dye eFluor™ 780 (ThermoFisher), anti-CD3 APC (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8α and a viability dye was added (Fixable Viability Dye-eFluor 506; Thermo Fisher Scientific). Subsequently cells were fixed and permeabilized (BD Cytofix&Cytoperm ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were isolated and stained with CD4-APC and CD90.1-PE (Invitrogen, 12-0900-83, 1:100) antibodies and analyzed using a NovoCyte flow cytometer ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells underwent intracellular staining using the Foxp3/Transcription Factor Staining Buffer Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD3 and CD4 surface markers (Live dead fixable stain #L34975, CD3-FITC #11-0032-82 from Invitrogen, USA and CD4-BV785 #100552 from BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... we labelled MACS-purified naïve CD4+ T cells with 5 μM CellTrace Violet (C34557; Invitrogen Molecular Probes) and then cultured the cells for 72 h in complete RPMI medium (RPMI medium supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... we labelled MACS-purified naïve CD4+ T cells with 5 μM CellTrace Violet (C34557; Invitrogen Molecular Probes) and then cultured the cells for 72 h in complete RPMI medium (RPMI medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified and genomic DNA was extracted according to TRIzol reagent protocol (15596018; ThermoFisher). The DNA was quantified with NANO-DROP spectrophotometer (Thermo-Scientific) ...
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bioRxiv - Immunology 2022Quote: ... at which point cells were collected and were stained with the following antibodies: CD4-PE (LOT:2004774, Invitrogen), RORγt-APC (LOT:2279171 ...
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bioRxiv - Biochemistry 2022Quote: ... Electroporation of plasmid DNA was conducted in resting CD4+ T cells by using Neon™ Transfection system (Invitrogen) through the suggested protocol for PBMC according to the manufacturers’ instructions ...