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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD4+CD25+ natural Tregs were isolated from the splenocytes using the DynabeadsFlowComp Mouse CD4+CD25+ Treg cells Kit (11463D, Invitrogen) following the manufacturer’s instructions and as previously described (37)(38) ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and CD4-positive spleen cells from the spleen with Dynabeads magnetic beads-conjugated CD4 antibody (clone L3T4; Thermo Fisher Scientific). Lysis buffer was immediately added to selected cells and total RNA was isolated using RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were stained overnight in 4 °C with rat-anti-CD4 (1:100, Invitrogen, 14-9766-82) and rabbit-anti-Iba1 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... cells in suspension were stained with PE-conjugated anti-CD4 antibodies (clone GK1.5, ThermoFisher Scientific, Waltham, MA, USA), followed by labelling with magnetic anti-PE MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... CD4 (RM4-5, Thermo Fisher Scientific, Waltham, MA), CD3 (145-2C11 ...
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bioRxiv - Neuroscience 2020Quote: ... CD4+cells were homogenized in TRIzol (Invitrogen, USA), and the total RNA was purified from the aqueous phase ...
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bioRxiv - Biochemistry 2020Quote: CD4 (Invitrogen, clone GK1.5, APC-eFluor780, 1:100), CD8 (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD4 (Invitrogen Cat # 25-0041-82, 1:500), and CD8 (Invitrogen Cat # 17-0081-82 ...
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bioRxiv - Immunology 2022Quote: ... CD4 (Thermo Fisher Scientific, Cat.No. 62-00420-80), CD11b(Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... and an APC-conjugated CD4 antibody (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4 FITC (Thermo Fisher, Waltham, MA, Clone GK1.5), CD4 PE Dazzle (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4-Alexa Flour 532 (ThermoFisher Scientific, Hillsboro, OR), CD8-PE-Cy7 ...
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bioRxiv - Immunology 2023Quote: ... CD4-AF488 (clone OKT4, Invitrogen #53-0048-42), CD8-APC (clone RPA-T8 ...
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bioRxiv - Immunology 2024Quote: ... CD4 e450 (1:200 Invitrogen, 48-0041-82), CD8a BV510 (1:200 BD Biociences ...
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bioRxiv - Developmental Biology 2022Quote: The Tregs and the CD4+/CD25- T cells were isolated from C57BL/6 mouse spleen using the Mouse CD4+CD25+ Treg Cells Kit (Invitrogen 11463D). The CD4+/CD8+ cells were sorted from testis ...
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bioRxiv - Immunology 2024Quote: ... primed NK cells from PWH were cultured with autologous CD4+ T cells also isolated by negative immunomagnetic selection (DynaBeads Human CD4+ T cell isolation kit, Life technologies) in the presence of 50nM Romidepsin (Selleck Chemicals ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using negative magnetic selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D).
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bioRxiv - Immunology 2024Quote: ... for the last 4h of culture and stained with anti-CD4 (PerCP-Cy5.5) anti-CD8 (APC) monoclonal Ab (Thermo Fisher Scientific, Waltham, MA, USA). Cells were fixed ...
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 30 minutes at 4°C in FACS buffer (PBS, 2% FBS, and 0.02% sodium azide) using anti-CD4 (clone GK1.5, eFluor 450 conjugate, ThermoFisher Scientific), anti-TCRα (anti-Vα11 ...
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bioRxiv - Immunology 2023Quote: Mice were intraperitoneally (i.p.) injected with 200 μg of rat anti-mouse CD4 monoclonal antibody (Clone GK1.5; ThermoFisher Scientific) 1 day prior to RICs immunization ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 30 minutes at 4°C in FACS buffer (PBS, 2% FBS, and 0.02% sodium azide) using anti-CD4 (clone GK1.5, eFluor 450 conjugate, ThermoFisher Scientific), anti-TCRα (anti-Vα11 ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Immunology 2024Quote: ... cells were surface-stained with antibodies against anti-human CD4 (PE-Cy5.5, clone: S3.5, Invitrogen, cat# MHCD0418, 1:100), anti-human CD8 (AF647 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in FACS buffer (PBS supplemented with 2% FBS and 5 mM EDTA) for 30 min at 4°C using the following antibodies: eFluor450 anti-mouse CD4 (GK1.5; Invitrogen), BV605 anti-mouse CD8 (53-6.7 ...
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bioRxiv - Biochemistry 2022Quote: ... Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2021Quote: Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific). Selected CD4+ T cells were activated by Dynabeads™ Human T-Activator CD3/CD28 kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... transduced cells were sorted and mixed with FACS sorted CD45.1+ naive CD4 T cells (CD4+ CD25− CD44Low CD62LHigh) labeled with CellTrace Violet (Thermo Fisher Scientific #C34571) in different ratio in the presence of irradiated T cell depleted spleen cells as antigen-presenting cells (APC) ...
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bioRxiv - Immunology 2024Quote: ... T cell proliferation was assessed by measuring CFSE-VPD450 dilution in CD4+ T cells (CD4-APC, Invitrogen, 17-0041-81, 1:100) and CD8+ T cells (CD8a-FITC ...
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bioRxiv - Immunology 2024Quote: ... transduced cells were sorted and mixed with FACS-sorted CD45.1+ naive CD4 T cells (CD4+ CD25− CD44lo CD62Lhi) labeled with CellTrace Violet (Thermo Fisher Scientific #C34571) in different ratios in the presence of irradiated T cell depleted spleen cells as antigen-presenting cells (APC) ...
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bioRxiv - Microbiology 2020Quote: ... which also encodes Cas9 and CD4 genes (Life Technologies). hFhl1 gene encoding FHL1A isoform fused with GFP was cloned into modified PiggyBac plasmid (System Bioscience ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were labelled with CellTrace Violet (Invitrogen) according to the manufacturer’s instructions prior to culture ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were isolated using positive selection (Invitrogen, 11331D), and cultured (1.25×106 cells/ml ...
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bioRxiv - Immunology 2020Quote: ... CD4 (GK1.5 or RM4-5, BioLegend or ThermoFisher Scientific), Bcl-6 (K112-91 ...
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bioRxiv - Immunology 2020Quote: ... Purified CD4+ T cells were cultured in DMEM (Invitrogen) T cell culture medium supplemented with 10% FCS (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ cells were isolated using positive selection (Invitrogen, 11331D), and cultured (1.25×106 cells/ml ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... Ter119 and CD4 antibodies (Thermo Fisher Scientific, Norcross, GA) or bone marrow c-Kit+LineagenegSca-1+ (KLS ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated by positive selection (Invitrogen, 11331D), and cultured at density 1.25×106 cells/ml in wells coated with anti-CD3 antibody (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... CD4 (SK3, BD, BUV469 / S3.5, Thermo Fisher, PE-Cy5.5), CD8 (SK1 ...
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bioRxiv - Bioengineering 2020Quote: ... 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen), FITC-conjugated anti-human CD4 (BD Bioscience) ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells were labelled with CFSE and stimulated with coated anti-CD3 (Thermo Fisher, #16-0037-85) (10 μg/mL).
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in 5% blocking serum at room temperature before being incubated overnight in the following primary antibody solutions (TBST, 1% serum): anti-CD4 (clone RM4-5, ThermoFisher), anti-CD8 (clone 4SM15 ...
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bioRxiv - Microbiology 2024Quote: ... large intestinal sections were incubated with either anti-CD4 (dilution 1:250; 14-9766-82, clone 4SM95, Thermo Fisher Scientific,), anti-CD8a (dilution 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were incubated for 10 min with 1 µl of an Alexa Fluor 647-conjugated anti-CD4 antibody (rat isotype, RM4-5, Invitrogen). The percentages of CD4-expressing cells were determined by FACS analysis using a BD Accuri C6 flow cytometer (Becton Dickinson) ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen nTreg and naïve CD4 were thawed and re-stimulated with anti-CD3/CD28 mAb-Dynabeads (Life Technologies, Carlsbad, CA) at 1:3 (cell to bead ...
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bioRxiv - Immunology 2021Quote: ... cells were first enriched using Dynabeads FlowComp mouse CD4 (Invitrogen), then stained with fluorochrome-conjugated CD45 ...
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bioRxiv - Cell Biology 2021Quote: ... and magnetic beads (Dynabeads® Untouched Mouse CD4 Cells, Invitrogen). Lineage-negative (Lin- ...
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bioRxiv - Immunology 2020Quote: ... and α-CD4–PE-Cy5.5 (clone S3.5) from Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Unstimulated naïve CD4+ T cells were cultured in IMDM (Gibco) with 5% heat-inactivated FBS ...