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Citations for Thermo Fisher :
3851 - 3900 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Eluting peptides were electrosprayed into the mass spectrometers (Exactive Plus EMR-Orbritrap or Q-exactive HF, both Thermo Fisher Scientific). Data were recorded using Xcalibur software (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... peptides were reconstituted in 6 μL of loading buffer (2% ACN, 0.1% TFA) and peptide concentration was estimated via optical measurement at 280 nm (Nanodrop 2000; Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Biochemistry 2020Quote: ... 1 nM of each acyl-peptide was added to 100 ng of a HeLa tryptic digest standard (Thermo Scientific, #PI88329) for a final ratio of 1 fmol acyl-peptide/100 ng of HeLa digest ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50-cm by 75-μm ID; ThermoFisher Scientific). The 125-min elution gradient at a constant flow rate of 300 nl/min was set to 5% phase B (0.1% formic acid in 99.9% acetonitrile ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were eluted from the column and electrosprayed into a Velos Pro Linear Ion Trap Mass Spectrometer (Thermo Fisher Scientific). Data was acquired using data-dependent acquisition (DDA ...
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bioRxiv - Immunology 2020Quote: ... Peptide stimulated and non-stimulated cells were first labeled with live/dead detection kit (Thermo Fisher Scientific, Waltham, MA, USA) and then resuspended in BD Fc Block (clone 2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of peptides was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Developmental Biology 2021Quote: ... The digested peptides were eluted on a capillary column and sprayed into a Q Exactive Mass Spectrometer (Thermo Fisher Scientific) equipped with a nano-ESI ion source (National institute of Bilogical Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated into eight fractions using the High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific Inc.). The fractions were dissolved in 0.1% formic acid ...
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bioRxiv - Immunology 2022Quote: The tryptic peptides were loaded onto an Acclaim PepMap 100 C18 trapping column(C18, 1.9 μm, 75 μm*20 cm; Thermo Fisher) at a flow rate of 200 nL/min ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS: Generated peptides were injected into a PepMap C18 5 mm x 300 um column (Thermo Fisher Scientific) at a flow of 25 μL/min with 0.1% TFA mobile phase ...
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bioRxiv - Cell Biology 2022Quote: ... LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Fisher Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 200ng of tryptic peptides were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using Ultimate 3000 UHPLC (ThermoFisher) connected to an Orbitrap Fusion Lumos Tribrid (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The dried peptides were resuspended in 200 mM EPPS pH 8.5 and quantified by bicinchoninic acid assay using the quantitative colorimetric peptide assay from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Digested peptides were analyzed by Orbitrap Fusion Lumos mass spectrometer coupled with EASY-nLC™ 1200 system (Thermo Fisher Scientific) for PRM ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Cross-linked peptides were analyzed by an Easy nanoLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) using data-dependent acquisition of ions with a charge state between 3 and 8 and HCD fragmentation using stepped normalized collision energy (NCE ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were first separated by an Ultimate 3000 RSLC nano and a Q-Exactive Plus mass spectrometer (both Thermo Scientific). Settings were set as described previously (https://pubmed.ncbi.nlm.nih.gov/30638812/) ...
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bioRxiv - Biochemistry 2022Quote: Peptides were resuspended in 0.5% formic acid and analysed using nanoflow LC-MS/MS using an Orbitrap Elite (Thermo Fisher) hybrid mass spectrometer equipped with a nanospray source ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were loaded onto a reversed phase trap column (Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Scientific) connected to the C18-reversed phase analytical column (EASY-Column ...
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bioRxiv - Microbiology 2022Quote: ... The peptide eluent from the C18 column was electrosprayed in Thermo Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, USA) in positive ion mode using 2.2 kV voltage ...
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bioRxiv - Immunology 2022Quote: ... Peptides were separated by reverse phase chromatography using a C18 pre-column (Acclaim PepMap 100, 75μm×2cm, Thermo Fisher Scientific) followed by C18 reversed-phase LC column (PepMap RSLC C18 ...
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bioRxiv - Immunology 2022Quote: ... 106 splenocytes were ex vivo stimulated with SARS-CoV-2 peptide pool (JPT PM-WCPV-S-1) at concentration of 2 μg/ml of each peptide in RPMI (Biowest) supplemented with 10% heat inactivated FBS (Gibco). PMA/Ionomycin (BD Pharmingen™ ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were first trapped onto a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with Buffer A at a flow rate of 2.5 µL/min over 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... fractions containing tryptic peptides were resolved and ionized using nanoflow UHPLC (Easy-nLC 1200 system, Thermo Fisher Scientific Inc., USA) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: ... The peptides were fractionated using high-pH HPLC methods and were then analyzed in Orbitrap Fusion™ Mass Spectrometer (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... Peptide analyses (corresponding to 1 µg protein) were performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) connected to an EASY-nLC 1200 ultra-HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... VTWFHAIHVSGTNGT31) immunodominant spike peptides (1 μg/mL) overnight at 37°C in the presence of Brefeldin A (1:500, Invitrogen). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on a 250 mm x 75 μm Acclaim PepMap® C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Neuroscience 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap® C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: Peptides for proteome analyses were separated by reverse phase liquid chromatography (LC) on an RSLCnano system (Ultimate 3000, Thermo Scientific) coupled online to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Carcasses were then incubated with 1:200 Alexa fluor 488-conjugated phalloidin (a bicyclic peptide that binds F-actin) (A12379, Invitrogen) for 40 minutes at 4°C and washed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... the peptide concentration for the two lowest temperatures was spectrophotometrically determined at 280 nm using a NanoDrop instrument (Thermo Scientific) and the average used as the concentration for all samples ...
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bioRxiv - Genetics 2022Quote: ... Peptides were collected by centrifugation and labeled using Tandem Mass Tag (TMTpro 16plex) reagents (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Desalted peptides were injected in an EASY-Spray™ PepMap™ RSLC C18 50cm X 75cm ID column (Thermo Scientific) connected to an Orbitrap Fusion™ Tribrid™ (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were first trapped onto a C18 column (75 µm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides (2 μg/ sample) were injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap Exploris Mass Spectrometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Non-deuterated samples peptide identification was performed via tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.1.2 (Sierra Analytics ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap™ 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were introduced using an EASY-Spray source at 2000 V to a Fusion Tribrid Orbitrap mass spectrometer (Thermo Scientific). Full MS spectra were recorded from 300 to 1500 m/z in the Orbitrap at 120,000 resolution with an automatic was performed using TopSpeed mode at a cycle time of 3 s ...
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bioRxiv - Cell Biology 2022Quote: ... The peptides were collected by centrifugation and isobaric labeling was performed using Tandem Mass Tag (TMT-10plex) reagents (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... peptides from each individual case and the GIS pooled standards were labeled using the TMTpro 16-plex kit (ThermoFisher 44520). Each batch in Set1 comprised 2 TMT channels with labeled GIS standards with all other channels reserved for individual brain samples ...