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Citations for Thermo Fisher :
4051 - 4100 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 72h of incubation the sections were incubated with secondary antibodies diluted in blocking solution for two hours: Alexa 594 anti-rabbit (Invitrogen, 1:1000), Alexa 488 High Cross Absorbed (Invitrogen ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... using primary antibodies diluted in blocking solution (VE-Cadherin, #14-1441-82, 5 μg/ml final and HES1, #PA5-28802, 5 μg/ml final, Thermo Fisher Scientific) for 48h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated for 2 hours at room temperature (22 ℃) in blocking solution containing the secondary antibodies Alexa Fluor® 555 donkey anti-rabbit (1:500, Thermo Fisher Scientific Cat# A-31570 ...
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bioRxiv - Neuroscience 2023Quote: ... After 1hr of incubation with blocking solution [0.1M tris buffered saline (TBS) with 1% bovine serum albumin (BSA, ThermoFisher Scientific, Waltham MA) and 5% dry milk] ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Coverslips were washed with blocking solution 3 times at room temperature and incubated in blocking buffer containing Alexa Flour 488 anti-mouse IgG antibody (1:300; Invitrogen, MA, USA) for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... The lower molecular weight part of the membrane was incubated overnight at 4ºC in anti-6X-HisTag antibody (1:1000 dilution in blocking buffer, Invitrogen MA1-21315-HRP) to stain for SsrB protein constructs ...
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bioRxiv - Neuroscience 2022Quote: ... Blots to be probed with the #1 antibody were blocked in 5% milk while all others were blocked in Superblock Blocking Buffer (ThermoFisher, Cat#37515), each with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with Odyssey Blocking Buffer (LiCor, USA) before being probed overnight with primary antibodies against C9ORF72 (1:1000, 22637-1-AP, Thermo Scientific, USA). Infrared labeled secondary antibodies were applied ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation in an Alexa Fluor (AF) 488-conjugated goat-anti-rabbit secondary antibodies in the blocking solution (dilution 1:200, ThermoFisher, A-11008) for 2 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ovaries were then stained for 12 hours using the following reagents at 1:500 dilution in blocking solution: Hoechst 33342 (10 mg/ml, Thermo Fisher H3570), Wheat Germ Agglutinin-647 (WGA ...
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bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... and then cells were washed with ice-cold blocking solution and incubated with secondary antibody (goat anti-rabbit with Alexa Fluor 555, 1:1000, Thermo Fisher Scientific). Cells were then washed with ice-cold PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... sections were washed for 5 minutes in PBS and immediately incubated in blocking solution for 30 minutes (Pierce Super Blocker, Thermo Fisher Scientific). Sections were then incubated in a primary antibody cocktail for 60 minutes containing 1x PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA Fraction V 7.5% (Thermo Fisher Scientific, 15260-037) + 5% goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer) for 1 hour at room temperature and detected using SuperSignal Western Dura PLUS Chemiluminescent Substrate (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody goat anti-Rabbit DyLight 680 (ThermoFisher, cat. no. 35568) or/and donkey anti-Goat DyLight 680 (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with blocking buffer and incubated with Alexa Fluor 594 or 568-conjugated streptavidin (ThermoFisher #S32356 or #S11226, 1:1000) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were incubated for 1 hour in blocking buffer containing goat αrabbit IgG (H+L) secondary antibody Alexa Fluor 568 conjugate (Thermo Fisher Scientific). Again ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206; or goat a-mouse Alexa488, Invitrogen A-11001) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed with TBST and incubated with secondary antibodies in blocking buffer [goat anti-rabbit (cat# A32731; Invitrogen, Waltham, MA, USA) or goat anti-mouse (cat# 8890 ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed utilizing the following horseradish peroxidase-conjugated primary antibodies that were diluted 1:1000 in blocking buffer: goat anti-mouse IgG1 (A10551, Thermo Fisher Scientific), goat anti-mouse IgG2c (PA1-29288 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2022Quote: ... in blocking buffer and then for 2 hours at room temperature with Alexa Fluor–conjugated secondary antibodies (1: 1000, Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were subsequently washed with PBS and incubated with fluorophore-conjugated secondary antibodies in blocking solution [anti-rabbit IgG Alexa Fluor™ 594 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of Brilliant Stain Buffer Plus (10 μl/sample) (cat# 563795, Becton Dickinson, Mountain View, Ca, USA) and CellBlox Blocking Buffer (5 μl/sample) (cat# C001T02F01, Thermo Fisher Scientific). Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401 ...
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bioRxiv - Synthetic Biology 2024Quote: ... All antibodies were diluted in blocking buffer as described in the Bioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00).
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:300 in the same blocking solution as well as the secondary antibody Alexa Fluor 647 (Cat. No. A21244, Life Technologies, USA). Plasma membranes were stained with wheat germ agglutinin (WGA ...
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bioRxiv - Genomics 2024Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific], Donkey anti-mouse Alexa Fluor 488 [A21202; Fisher Scientific] ...
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 hour at room temperature and subsequently incubated in the blocking buffer containing rabbit anti-SYNGAP1 (1:5000; ThermoFisher #PA1-046), rabbit anti-SRGAP2A (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then exposed at room temperature first for 1 hour to primary Abs in blocking buffer and then for 30 minutes to Alexa Fluor–conjugated secondary Abs (Thermo Fisher Scientific) in blocking buffer ...