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Citations for Thermo Fisher :
3651 - 3700 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4 °C: AT8 (THERMO SCIENTIFIC, MOUSE,1:100), IBA1(WAKO ...
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bioRxiv - Neuroscience 2024Quote: The blocking solution for all immunostaining approaches was 1X PBS (no Ca2+ / Mg2+) with 1% normal donkey serum (Fisher Scientific; OB003001), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were stained directly using a nanobody against ALFA tag coupled to a Abberior Star 635P fluorophore (FluoTag®-X2 anti-ALFA - AbberiorStar635P, NanoTag Biotechnologies GmbH) diluted 1: 500 in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed with PBS-Tween and PBS and incubated 1 hour with appropriate secondary antibodies in blocking solution (1:500, AlexaFluor594, AlexaFluor488, Invitrogen, UK) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... they were rinsed three times in PBS and blocked for 30 minutes in blocking solution (PBS + 5% by volume normal goat serum, ThermoFisher PCN5000). Staining was for 1 hour with 1:500 anti-OSBP (Atlas Antibodies HPA039227 ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells incubated with secondary antibodies in blocking solution for 1h at RT in the dark (anti-chicken Alexa 568, 1:1000, A11041, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... at room temperature in blocking buffer containing the goat anti-rabbit Alexa Fluor 568 conjugate (1:250, Life Technologies #A-11011) secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with PBS and incubated with Alexa Fluor-488 conjugated goat anti-mouse IgG secondary antibody diluted at 1:500 in blocking solution (Invitrogen, USA). Fluorescent foci were visually counted (in a well with a dilution that produced <60 total foci ...
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bioRxiv - Neuroscience 2022Quote: ... then processed for 48 hours at 4 degrees with secondary antibodies in blocking solution (1:1000, Alexa Fluor 647 goat anti-rabbit, Jackson Laboratories, plus 1:500 NeuroTrace, Invitrogen). Tissue sections were then rinsed again 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2023Quote: Immunohistochemistry was performed as follows: brain sections were incubated in a blocking solution consisting of 5% normal goat serum (Thermo Fisher) and 0.1% Triton (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep antibodies were incubated for 1h and after thorough washing and blocking with 5% normal goat serum (Life Technologies) for 30 min ...
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bioRxiv - Genomics 2023Quote: ... Then DNA oligo-conjugated primary antibodies were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were diluted with odyssey blocking buffer in tris-buffered-saline containing 0.1% Tween20 (TBS-T) (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... + 0.05% Tween 20 and blocked with 4% milk powder in PBS (Regilait) in the first panning round and Pierce protein free blocking buffer (Thermo Scientific), SEA blocking buffer (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... representative brain sections were wasted three times in 1× PBS and incubated in a 24 well plate at room temperature for one hour in blocking buffer: 10% goat serum (Gibco, 16210064), 1% BSA (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized with 0.01% Triton X-100 in PBS for 5 min at room temperature and then treated with BlockAid Blocking Solution (Thermo Fisher) supplemented with FcR Blocking Reagent (Miltenyi ...
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bioRxiv - Biochemistry 2023Quote: ... UV-crosslinked at 120 mJ and blocked for 2 hours rotating at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Thermo Fisher). Blots were probed rotating overnight at 42°C with 32P-labeled DNA probes prepared as described below ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 in blocking buffer for 1h and counterstained with DAPI prior to mounting with ProLong Gold anti-fade reagent (Invitrogen, P36930) for subsequent immunofluorescence analysis.
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bioRxiv - Cancer Biology 2023Quote: ... The lentiviral constructs pHIV-dTomato-CDK19_CasR and pHIV-dTomato-CDK19M_CasR that contain the coding sequences of human CDK19-wild-type or CDK19-D173A kinase-inactive mutant with mutations introduced in the sgRNA-targeting sequence and proximal PAM sequences (blocking sgRNA targeting but not changing the coded protein sequences) were generated using the Phusion Site-Directed Mutagenesis Kit (Thermo Scientific) with the hCDK19-CasR-F/R primers (Table S5 ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Biophysics 2023Quote: ... samples were blocked for 15 min in blocking buffer and then incubated with Alexa Fluor conjugated primary antibody against phospho-Src (Tyr419) (44-660G, ThermoFisher Scientific). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for one hour at room temperature with blocking buffer containing secondary antibodies for Pax7 (ThermoFisher #A-11072; 1:500) and MyoD (ThermoFisher #A-11001) ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with peroxidase-linked secondary antibodies for 1h at RT diluted in blocking buffer and then treated with Supersignal West Pico solution (Thermo Scientific). Images were acquired on an LAS-3000 system (Fuji Film).
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody was diluted in blocking solution and added to cells (30 minutes, room temperature) with phalloidin-AlexaFluor568 (1:100 dilution, Invitrogen, A12380) included when indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were treated at 4 °C overnight with the following primary antibodies in blocking buffer: anti-LCP1 (MA5-11921; Thermo Fisher); anti-Vinculin (MAB3574 ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 10 minutes at room temperature and underwent a blocking step with 0.05% (v/v) Triton X-100 and 10% (v/v) normal goat serum (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibody (diluted in the blocking solution) was used: rabbit anti–PGP9.5 (1:400, Thermo Fisher Scientific, #PA5-29012), rabbit anti-S100β (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in blocking buffer containing secondary antibodies (Alexa Fluor 555 donkey anti-rabbit IgG, 1:500 dilution, A31572, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were blocked in blocking buffer (DPBS containing no Ca2+ or Mg2+ and supplemented with 3% dialyzed FBS; Thermo Fisher). For EC analysis ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Then sections were blocked with a blocking solution (PB supplemented with 0.3% Triton X-100 and 5% normal goat serum, NGS, Gibco, 16210-064) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated with secondary antibody diluted in blocking solution: Goat anti-Rabbit IgG Alexa-488 (Invitrogen A-11008), Goat anti-Rat IgG Alexa 594 (Invitrogen A-11007) ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking was carried out for 15 minutes at room temperature using a blocking solution that included 10 mg/mL UltraPure BSA (Invitrogen AM2616), 0.3% Triton-X ...
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bioRxiv - Systems Biology 2023Quote: ... the cells were blocked and permeabilized for 30 min at RT in a mix of 0.5x PFBB (protein free blocking buffer, Thermo Fisher Scientific) in PBS supplemented with 10 µg/ml tRNA (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed four times in 0.1 M PBS with 0.5% Triton X-100 for 5-10 minutes each and then incubated in blocking solution containing 1:400 secondary antibody donkey Polyclonal anti-rabbit Alexa Fluor Plus 488 (A32790, ThermoFisher Scientific) for 2.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed three times with PBS and incubated for 1 h at room temperature with secondary antibodies diluted in blocking buffer (Alexa fluor (AF) 488 donkey anti-rabbit (1:500; ThermoFisher Scientific), AF568 donkey-anti-rat (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...