Labshake search
Citations for Thermo Fisher :
4101 - 4150 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... slides were washed in PBS for 5 minutes and subsequently incubated in blocking solution for 15 minutes (Pierce Super Blocker; Thermo Fisher Scientific). The slides were incubated in primary antibody solution (1x PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... for 1 h at room temperature preceding primary antibody incubation using a 1:1 mixture of Odyssey Blocking Buffer and TBST (ThermoFisher, J77500.K8) diluted to 1x concentration at 4°C overnight ...
-
bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
-
bioRxiv - Neuroscience 2024Quote: The slides were pre-incubated in blocking solution and stained against Griffonia simplicifolia isolectin GS-IB4 Alexa 568 (Invitrogen, I21412; 1:500), Neurofilament 200 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... The hydrated slide is blocked in 3% skim milk PBST for 1 hour and incubated with primary antibody (1:100) diluted in Superblock Blocking Buffer in TBS (#37535, Thermo Fisher Scientific) at room temperature overnight ...
-
bioRxiv - Plant Biology 2019Quote: ... Two μL (~1μg) peptides were analyzed by LC-MS with a Dionex RSLCnano HPLC coupled to a LTQ-Orbitrap Velos Pro (Thermo Scientific) mass spectrometer using a 2 h gradient ...
-
bioRxiv - Biophysics 2021Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
-
bioRxiv - Biophysics 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Cancer Biology 2021Quote: Peptides were separated on an EASY-Spray™ Acclaim PepMap C18 column (50 cm × 75 μm inner diameter, Thermo Fisher Scientific) using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A ...
-
bioRxiv - Cancer Biology 2021Quote: ... The peptides were analyzed on a LC-MS/MS platform consisting of an Easy-nLC 1200 UHPLC system (Thermo Fisher Scientific) interfaced with an QExactive HF orbitrap mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... the extracted peptides were completely dried to completion and thereafter desalted using the SPE Pierce C18 Spin Columns (Thermo Fisher Scientific). These columns were activated by 2 × 200 μL of 50% acetonitrile (CAN ...
-
bioRxiv - Molecular Biology 2021Quote: ... samples were passed through an immobilized pepsin column (2 mm□× 2 cm) at 120 μL min−1 and the digested peptides were captured on a C18 PepMap300 trap column (Thermo Fisher) and desalted ...
-
bioRxiv - Developmental Biology 2020Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described (36).
-
bioRxiv - Molecular Biology 2022Quote: ... Target peptides for each of these proteins were purchased in stable isotope labelled synthetic peptides (SIS) form in crude quality from Thermo Scientific. The C-terminal lysine or arginine in the SIS peptides were replaced by isotope labelled lysine (13C6 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
-
bioRxiv - Cell Biology 2022Quote: Fractions of crosslinked peptides were analyzed by liquid chromatography coupled to tandem mass spectrometry using an LTQ Orbitrap Elite (Thermo Scientific) instrument 65 ...
-
bioRxiv - Biochemistry 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Immunology 2019Quote: ... and resulting peptides were fractionated and sequenced by nanoflow LC-electrospray MS/MS on an Orbitrap Velos Pro hybrid mass spectrometer (Thermo Scientific), in the UT Austin mass spectrometry core facility as described previously (26) ...
-
bioRxiv - Molecular Biology 2020Quote: ... High pH reversed fractionation was achieved by attaching a XBridge Peptide BEH C18 column (Cat. 186003570; Waters Corp.) to an Accela liquid chromatography system (Thermo Scientific) using the HPLC application ...
-
bioRxiv - Molecular Biology 2020Quote: ... The column was enclosed into a column oven (Sonation) and the peptides were sprayed into a Q Exactive Plus mass spectrometer (Thermo Fisher). The mass spectrometer was operated in a data-dependent acquisition mode using a top10 method ...
-
bioRxiv - Immunology 2022Quote: The encapsulation efficiency of the peptide into the NLC formulation was determined via the CBQCA protein quantitation kit (Thermo Fisher Scientific). Three separate NLC peptide formulations were created using the optimized formula previously determined in the DOE ...
-
bioRxiv - Genomics 2020Quote: ... Flag peptide and small size of non-specific proteins were removed by 20K Slide-A-Lyzer dialysis cassette (ThermoFisher Scientific, 66003) with 1L binding buffer (PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to Sequelon-AA membrane (Applied Biosystems) as described previously [40].
-
bioRxiv - Immunology 2019Quote: ... prior to injection of 2 µg of purified peptides into an Ultimate3000 nano-LC system coupled to a Q Exactive mass spectrometer (ThermoFisher Scientific) for mass spectrometry ...
-
bioRxiv - Genetics 2020Quote: ... Peptide mixtures were analysed by nLC-ESI-MS/MS on a LTQ-Orbitrap XL coupled to an Easy nLC (Thermo Scientific). The sample preparation and the nLC-ESI-MS/MS analysis were performed as previously described (Rappsilber et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... followed by MS measurement of peptides and their fragmentation spectra (LC-MS/MS) with a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific).
-
bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
-
bioRxiv - Biochemistry 2019Quote: The peptide samples resulting from the above digestion were separated by an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, USA). First ...
-
bioRxiv - Plant Biology 2019Quote: The separated peptides were analyzed on-line by using a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operated in standard data-dependent acquisition mode controlled by Xcalibur version 4.0.27.19 ...
-
bioRxiv - Synthetic Biology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1575 m/z were performed at 120K resolution (at 200 m/z ...
-
bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
-
bioRxiv - Cell Biology 2019Quote: ... in accordance with the manufacturer’s instructions and then fractionated using a Pierce high-pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Eight fractions were collected ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Peptides were separated on a column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, Thermo Fisher Scientific). The column had a 100 μm inner diameter microcapillary ...
-
bioRxiv - Molecular Biology 2019Quote: Peptide fractions from the small-scale high pH reversed phase separation were analysed on an Orbitrap Tribrid Fusion (Thermo Fisher Scientific) with the same MS parameters as described above ...
-
bioRxiv - Developmental Biology 2020Quote: ... Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
-
bioRxiv - Cell Biology 2019Quote: ... Extracted peptides were desalted and used for LC-MS/MS data acquisition on an Orbitrap Luminos mass spectrometer (Thermo Fisher Scientific) coupled with a 3000 Ultimate high-pressure liquid chromatography instrument (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2019Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 ml/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... Eluted peptides were subjected to electrospray ionization coupled to an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo-Fisher Scientific) to produce tandem mass spectra whose b-and y-ion series patterns were compared to theoretical digests of the proteome in Sequest (Thermo-Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... PBMC from HLA-A2 individuals were isolated by Ficoll-Hypaque density gradient centrifugation and co-cultured with 40 μM EBV peptide for 12 days in complete medium supplemented with 1% sodium pyruvate (Gibco, UK) plus 10% human serum (Sigma,UK) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Then the peptides were separated with high-performance liquid chromatography (HPLC, Ultimate 3000 Nano LC System, Thermo Scientific, Rockwell, IL, USA) in a 15-cm long C18 column (Acclaim® PepMap™ RSLC inner diameter of 75 μm ...
-
bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... and buffer B (80 % acetonitrile, 0.1 % formic acid) was used for peptide separation on an Easy-nLC 1200 (Thermo Fisher Scientific). This system was coupled via a nano electrospray ionization source to the quadrupole-based Q Exactive HF benchtop mass spectrometer 43 ...
-
bioRxiv - Biochemistry 2021Quote: ... a parallel reaction monitoring regime (PRM) was used to select for a set of previously selected peptides on an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific) operating in positive mode and running Tune version 3.3 ...
-
bioRxiv - Genetics 2021Quote: ... by rotating at room temperature for 1h and washed 3x with extraction buffer and protein was eluted 3x with 300 ul of 1 mg/mL HA peptide (ThermoFisher, 26184) in 1x TBS ...
-
bioRxiv - Genomics 2021Quote: ... and 10 µg proteins were proteolysed with trypsin and peptides analysed by quantitative LC-MSMS on a Q Exactive HF (Thermo Fisher) by a data-independent acquisition approach as described earlier86 ...
-
bioRxiv - Evolutionary Biology 2021Quote: Peptides were analysed on a Dionex Ultimate 3000 RSLCnano system coupled to a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). For full proteome analyses ...
-
bioRxiv - Immunology 2021Quote: ... ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher) was determined by AmplexRed assay [3] ...
-
bioRxiv - Plant Biology 2020Quote: ... Eluates containing the enriched phosphopeptides were dried down and subsequently resuspended with 50 μl 0.1% formic acid for peptide concentration measurement using the Pierce Quantitative Colorimetric Assay kit (Thermo Scientific PN23275).
-
bioRxiv - Neuroscience 2020Quote: ... Peptides eluted from the nano LC column were subsequently ionized into the Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific). The electrospray source was fitted with an emitter tip 10μm (New Objective ...