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Citations for Thermo Fisher :
3751 - 3800 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Separated peptides were directly eluted into a nanospray ion source of an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific). The stainless steel emitter spray voltage was set to 2200 V ...
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bioRxiv - Biochemistry 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Data-dependent LC-MS/MS analysis of extracted peptide mixtures after digestion was carried out with a LTQ-Velos PRO orbitrap mass spectrometer (ThermoFisher) interfaced to a nanocapillary HPLC (RSLC3000 ...
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bioRxiv - Cell Biology 2021Quote: The triptic peptides were analyzed by a LTQ XL-Orbitrap ETD equipped with a HPLC NanoEasy-PROXEON (Thermo Fisher Scientific). They were loaded ...
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bioRxiv - Biochemistry 2021Quote: ... The desalted tryptic peptide samples were loaded onto an Acclaim PepMap 100 pre-column (75 μm x 2cm, ThermoFisher Scientific) and separated by Easy-Spray PepMap RSLC C18 column with an emitter (2 μm particle size ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant containing the peptides was collected and dried down in a centrifugal evaporator (Savant SPD 1010, Thermo scientific). The peptide mixtures were reconstituted in a solution of 2% (v/v ...
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bioRxiv - Physiology 2020Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2021Quote: Desalted peptides were analyzed by liquid chromatography coupled mass spectrometry using a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific), coupled online to a RSLC nano HPLC (Ultimate 3000 ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were trapped and desalted on a C18-column (5 μm Acclaim PepMap100 300 μm x 5 mm, ThermoFisher Scientific) at a flow rate of 30 μl/min with solution A (1% acetonitrile (ACN) ...
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bioRxiv - Cell Biology 2021Quote: ... Desalted peptides were dissolved in 0.1% (v/v) formic acid and injected onto an Easy-nLC 1000 (Thermo Fisher Scientific), coupled to an Orbitrap Exploris 480 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were then analyzed by LC-MS/MS using a LTQ-Orbitrap Fusion Tribride mass spectrometer (Thermo Fisher Scientific). Peptide identification was performed using Proteome Discoverer v1.4.1.14 (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Specific peptide sequences unique for the target proteins (AtpA, AtpB, AtpE) were created using the Pinpoint software (version 1.0, Thermo Scientific) and are listed in Supplemental Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... by EASYnLC and the peptides separated through an Acclaim PepMap RSLC column (0.075 mm x 150 mm C18, Thermo Scientific) with the same gradient as above ...
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bioRxiv - Systems Biology 2020Quote: ... Desalted peptides were reconstituted in 50 mM HEPES (pH 8.5) and labelled with TMT10plex158 Isobaric Label Reagent (Thermo Fisher Scientific) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were eluted with a 60% acetonitrile (ACN)/0.1% TFA solution and dried in a SpeedVac vacuum concentrator (Thermo Fisher). For the IP of di-glycine peptides ...
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bioRxiv - Neuroscience 2020Quote: ... A spectral library was created in Skyline for some of the target peptides using several DDA of FMRpolyG samples as well as blanks spiked with AQUA Ultimate endogenous (light) and isotopically labeled (heavy) peptides obtained from ThermoFisher Scientific (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were infused directly from the column tip into the on-line coupled Q Exactive HF mass spectrometer (Thermo Scientific) using nanoelectrospray ion source (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The chromatographic separation of the peptides was achieved using a 50 cm long C18 Easy spray column (Thermo Scientific,US) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL/min flow rate) was used for peptide separation right followed by LTQ-FT mass spectrometry analysis (ThermoFisher Scientific) with data acquisition at a mass resolving power of 100,000 at m/z 400 ...
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bioRxiv - Microbiology 2020Quote: ... peptides were recovered in HEPES buffer by collecting supernatant on magnet and combining it with a second elution wash of the magnetic beads with HEPES buffer. Peptides were labelled with TMT10plex (Werner et al. 2014) Isobaric Label Reagent (ThermoFisher) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The digested peptides were analyzed by reverse phase LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a Dionex UltiMate 3000 -nLC (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were then introduced by nanoelectrospray into a LTQ Orbitrap Velos Pro coupled to an Easy-nLC HPLC (Thermo Fisher) over a linear gradient of acetonitrile at 250 nl/min over 140 min ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides from each sample were labelled using the ten-plex TMT reagent kit (90110, Thermo Fisher Scientific, Waltham, MA, US). Two ten-plex experiments were performed ...
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bioRxiv - Biophysics 2020Quote: ... or RGD-containing peptide (Peptite-2000; 100 μg mL−1 in PBS) in 1× PBS with Ca2+ (DPBS, Catalog No. 14040133, Gibco). Unattached beads were removed by washing with 1× PBS.
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bioRxiv - Biophysics 2021Quote: ... were vacuum-dried and resuspended with the peptide fractionation buffer [(70% (v/v) LC-MS grade water (Thermo Fisher Scientific), 30% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The first set of LC-MS/MS was used to identify highly abundant unoxidized peptides using the Sequest search engine (Proteome Discoverer 1.4, Thermo Scientific) against the Uniprot Rattus Norvegicus database (downloaded on March ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting peptides were trapped and desalted on a 2 mmL×L10 mm C8 trap column (Hypersil Gold, ThermoFisher). The bound peptides were then gradient-eluted (4-40% CH3CN v/v and 0.3% v/v formic acid ...
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bioRxiv - Cancer Biology 2022Quote: Peptides were injected onto a trapping column for preconcentration (Acclaim Pepmap100 20cm x 75 μm, 3μm C18, Thermo Fisher Scientific), followed by nanoflow LC (Thermo Dionex ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Identifications and retention times were used to guide the manual quantification of each modified peptide using QualBrowser version 2.0.7 (Thermo Fisher Scientific). Site assignment was evaluated from MS2 spectra using QualBrowser and MaxQuant Viewer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted peptides were then subjected to electrospray ionization directly coupled to a high-resolution Orbitrap mass spectrometer (QExactive, ThermoFisher).
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Neuroscience 2022Quote: ... Constant flow of 10 μl/min was maintained for peptide loading onto a pre-column (PepMap 100; Thermo Fisher Scientific). Peptides were then separated on a 10 mm PicoTip™ fused silica emitter 75 μm x 16 cm (New Objective Inc. ...
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bioRxiv - Molecular Biology 2022Quote: Digested peptides were acidified with 0.8% TFA (final) and analyzed by LC–MS/MS on an EASY-nLC 1000 (Thermo Scientific) with a two-column set-up ...
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bioRxiv - Pathology 2022Quote: ... Then peptide mixtures of each sample were labeled using the TMTsixplex reagent according to the manufacturer’s instructions (Thermo Fisher Scientific). Samples were labeled with the TMT tags ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Biophysics 2022Quote: The GyrA14-E487C and the CcdA50-72 peptide (Table S1) were labeled with Fluorescein-5-Maleimide and 5-TAMRA (Tetramethylrhodamine-5-Maleimide) from ThermoFisher Scientific as per the manufacturer’s protocol as described previously (Aghera et al. ...
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bioRxiv - Microbiology 2022Quote: ... the LC-MS/MS data were used as the input for peptide identification using the database search engine SEQUEST HT implemented in Proteome Discoverer 2.2 (Thermo Scientific). Note ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatographic separation of the peptides was performed by liquid chromatography on a nano-UHPLC system (Ultimate UHPLC focused, Thermo Scientific) using a nano column Acclaim PepMapC100 C18 ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Fractionated peptides were ionized and analyzed by tandem mass spectrometry (MS/MS) Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Label-free quantitation was performed using the MaxQuant software package.79 The EMBL Human reference proteome (UP000005640 9606 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded first onto an Acclaim PepMap 100 Trapping column (Thermo Scientific, 20 mm 75 µm inner diameter (ID), 3 µm of C18 resin with 100 Å pore size ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli digests and Promega yeast protein digest were each fractionated by offline reversed-phase LC (Waters Acquity UPLC Peptide BEH300 C18 1.7 µm, 1 mm i.d. x 150 mm column employing an Ultimate 3000 HPLC, Thermo Fisher Scientific) operating with column compartment temperature of 60 °C and flow rate of 100 µL/min ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was affinity purified on phospho-peptide covalently bound to a SulfoLink column according to the manufacturer’s instructions (Thermo Scientific).