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Citations for Thermo Fisher :
3551 - 3600 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Human ASGR1 and its mutants (Q240A/W244A and Q240A/W244A/E253A) and human ASGR2 were sub-cloned into pcDNA 3.1 vector (Invitrogen) containing a C-terminal Flag tag (ASGR1 ...
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bioRxiv - Synthetic Biology 2021Quote: Flp-In™ T-REx™ Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (Catlog# R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Developmental Biology 2021Quote: A human episomal iPSC line derived from CD34+ cord blood was used in this study (A18945, ThermoFisher Scientific; [58]). This cell line has already been validated in its ability to generate light-sensitive 3D retinal organoids ...
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of alkaline phosphatase-conjugated goat anti-human F(ab’)2 antibody (Thermo Fisher Scientific) Inc. ...
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bioRxiv - Immunology 2021Quote: Cells from the human cancer cell line Colo205 were cultivated in complete RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 2 mM L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: The viral-integration-free human iPSC line generated using cord blood-derived from CD34+ progenitors was obtained from Gibco™/Thermo Scientific (Cat ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Cancer Biology 2020Quote: Human T-ALL cell lines (ARR, DU.528, HSB2, and CCRF-CEM) were cultured in RPMI 1640 medium (Gibco), supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS ...
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bioRxiv - Evolutionary Biology 2021Quote: Human ESCs (WA01, also called H1, from WiCell Research Institute) were cultured with StemFlex Medium (Gibco, Cat. No. A3349401) in a feeder cell-free condition ...
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bioRxiv - Cancer Biology 2021Quote: Human breast cancer cell line MDA-MB-468 cells were cultured in Leibovitz L-15 medium (Gibco #21083-027) supplemented with 10% heat-inactivated FBS and 1xpenicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... was introduced to primary human umbilical vein endothelial cells (HUVEC) using the Neon® transfection system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Human telomerase-immortalised retinal pigment epithelial cells (hTERT-RPE1, ATCC) were grown in DMEM-F12 (Life Technologies, Paisley, UK) supplemented with 10% decomplemented FCS (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Human alpha thrombin (Nordic Diagnostica AB, Billdal, Sweden) was resuspended in OptiMEM reduced serum medium (RSM) (Thermo Fisher Scientific) to 1 U/μl and stored at −20 °C ...
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bioRxiv - Biochemistry 2020Quote: The human colon cancer cell line HCT 116 was grown in McCoy’s 5A medium with glutamax (GIBCO, 36600–021) to which 10% fetal calf serum (FCS ...
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bioRxiv - Bioengineering 2021Quote: ... Briefly, THP-1 human monocytes (ATCC®, Manassas, VA) were cultured in complete RPMI media (ThermoFisher Scientific, Waltham, MA), supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (37) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h before opsonization with human IgG or 200 µg/mL anti-BSA antibody (Invitrogen, Burlington, ON, Canada). RAW cells were pre-cooled to 4°C for 5 min prior to the cells being presented with targets at ratios of 20-400 rods per cell ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolates (Human/NL/Lelystad/2020) were propagated in Vero E6 cells (ATCC) in Eagle’s minimal essential medium (Gibco) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... Asexual parasites were cultured in human blood (UK National Blood Transfusion Service) and RPMI 1640 medium containing 0.5% w/v AlbumaxII (Invitrogen) at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgM and IgG conjugates provided within the kit were replaced with anti-mouse IgM-HRP-labeled (Invitrogen) and anti-mouse IgG-HRP-labeled (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... human embryonic kidney cells (HEK) 293T were grown in a T75 flask with Dulbecco’s modified eagle medium (DMEM) (Gibco) + 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were maintained by growth on monolayers of human foreskin fibroblasts (HFF) in Dulbecco’s Modified Eagles Medium (DMEM) containing 10% Cosmic Calf Serum (GIBCO), 2 mM Glutamine ...
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bioRxiv - Immunology 2020Quote: ... the fixed sample was incubated with 1:500 dilution of goat anti-human coupled to Alexa 633 fluorophore (Invitrogen) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2021Quote: Cytokines in AqH samples were quantified using a ProcartaPlex Human Cytokine-Panel 1B (Thermo Fisher Scientific, Waltham Massachusetts, USA) that quantifies ...
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bioRxiv - Immunology 2021Quote: Human CD4 T cells were MACS-sorted from PBMC and subsequently pre-activated with α-CD3/CD28 Dynabeads (Thermofisher) at a bead-to-cell ratio of 1:1 for 48 hours ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μL of a secondary Alexa Fluor 647 goat anti-human antibody (Life Technologies A21455) (1:500 in PBS with 0.1% (w/v ...
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bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
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bioRxiv - Genomics 2021Quote: ... Naive and memory T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 beads-to-cells ratio ...
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bioRxiv - Genomics 2021Quote: ... RNA for gene expression analysis from primary human pseudoislets was extracted using the PicoPure RNA isolation kit (Life Technologies) and cDNA was synthesized using the Maxima first strand cDNA synthesis kit (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: Human iPSCs from WTC11 and NCRM5 backgrounds3 were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401) in plates or dishes coated with Growth Factor Reduced ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Genetics 2021Quote: ... were genotyped using the Affymetrix Genome-Wide Human SNP Array 5.0 (SNP5) chip (Affymetrix Inc., Santa Clara, CA, USA). For the Athero-Express Genomics Study 2 (AEGS2) ...
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bioRxiv - Genomics 2020Quote: The cells The human embryonic kidney 293T (HEK293T) cell were cultured in DMEM containing 10% FBS (Thermo Fisher Scientific), 100 U/ml penicillin (A603460 ...
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bioRxiv - Microbiology 2020Quote: ... and human umbilical vein endothelial HUV-EC-C cells (ATCC; CRL-1730) in RPMI 1640 medium (Gibco; #11875-093) containing 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Immunology 2022Quote: ... Human THP-1 monocytes (ATCC, TIB-202) were grown in RPMI 1640 Medium with ATCC modification (Thermo Fisher Scientific) supplemented with 10% FBS and 50 μM 2-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: Human embryonic genital ridge or mesonephros from a week 7.0 male embryo were collected in dissection medium (DMEM (Gibco), 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... When HIV was reactivated using Dynabeads Human T activator CD3/CD28 for T cell expansion and activation (ThermoFisher Scientific), beads were magnetically removed the next day ...
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bioRxiv - Cell Biology 2022Quote: ... human embryonic kidney 293T cells (HEK; CRL-3216; ATCC; RRID: CVCL_0063) were cultured in DMEM media (12430062; Gibco™) with 10% FBS (098-150 ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies [Alexa 647-goat anti-mouse or Alexa 647 Goat anti-Human IgG (H+L) Cross-Adsorbed (Invitrogen)] at 1:1000 dilution were then incubated for 60 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: Large-scale production of bio-inactive human N-SLIT2ΔD2 was performed by transfecting HEK293F cells with human N-SLIT2ΔD2 cDNA (1 ug/mL) using PEI reagent (Polyethyleneimine, linear, M.W. 25,000, Thermo Fisher Scientific) (19) ...
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bioRxiv - Immunology 2022Quote: ... Wells were then incubated with a secondary goat anti-human IgG labeled with horseradish peroxidase (HRP) (1:5000 Invitrogen) TMB substrate (SIGMA) ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...