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Citations for Thermo Fisher :
3401 - 3450 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Immunology 2020Quote: ... The human monocytic THP-1 cell line was maintained in RPMI-1640 (made inhouse, RPMI 1640 powder (Life Technologies), 23.8mM Sodium Bicarbonate (NaHCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.3 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 10 ppm and 0.6 Da respectively ...
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bioRxiv - Genetics 2019Quote: ... to distinguish recent and distant relatedness in the sample using markers from the Genome-Wide Human SNP 6.0 array (Affymetrix). The percent of total variation in height and BMI was estimated using the coefficient of determination (R2 ...
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bioRxiv - Developmental Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of geltrex (Gibco, A14133-01), in E8 Basal Medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Unlabeled and ferumoxytol-labeled human MSCs were tested for tri-lineage differentiation potential using StemPro differentiation kits (Life technologies) for adipogeneic ...
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bioRxiv - Genomics 2019Quote: ... The HUES9 human embryonic stem cell line was grown on tissue culture plates coated wth Geltrex (Thermo Fisher Scientific) in mTeSR1 media (StemCell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.2 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 7 ppm and 0.6 Da respectively ...
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bioRxiv - Developmental Biology 2019Quote: DNA sequences corresponding to the human Amyloidβ1-42 amino acid sequence were synthesized (IDT) into a Gateway technology (Invitrogen) entry vector ...
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bioRxiv - Microbiology 2019Quote: ... and human glioblastoma cells (U-87MG, ATCC no.: HTB-14) were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies). Chinese hamster ovary cells (CHO-K1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The labeled samples were then hybridized to Affymetrix Human Genome U133 Plus 2.0 Array GeneChips (ThermoFisher Scientific, Waltham MA) and scanned Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA from sorted CXCR3+ or CXCR3− SUM-LM1 cells was analyzed using Affymetrix Human U133 Plus 2.0 microarrays after cDNA preamplification using the 3’IVT Pico Reagent Kit (Affymetrix). To generate the CXCR3 signature ...
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bioRxiv - Cancer Biology 2019Quote: The immortalised non-tumorigenic human epithelial cell line MCF12A (ATCC, CRL-10782),90 were cultured in DMEM/F12 (Gibco) supplemented with 5% horse serum (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Swabs were screened for common human respiratory pathogens using a customized TaqMan Array Card® (ThermoFisher, Waltham, MA, USA), which included influenza A and B viruses ...
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bioRxiv - Immunology 2021Quote: ... NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes) of human complete MSC medium (RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney (HEK)-293 cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11965-092) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells and the Jurkat.E6 cell line were cultured in RPMI-1640 (Thermo Fisher Scientific; catalog #11875119). All media were supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Stealth RNAiTM siRNAs Negative Control, Med GC and human Il11, NM_000641.3 (HSS179893, HSS179894, HSS179895) were purchased from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2021Quote: DNA sequence coding for human Xkr8 (hXkr8) (UniProt: Q9H6D3) was custom-designed and synthesized by GeneArt (Thermo Fisher Scientific) to enhance mRNA stability and translational efficiency ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... and MDA-MB-231 (ATCC HTB-26) human breast cancer cells were cultured in medium composed from standard Dulbecco’s Modified Eagle Medium (DMEM, Gibco), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... Human ACE2 ectodomain (NCBI Reference Sequence: NP_001358344.1, residues Q18-S740) was expressed in Expi293™ cells (Thermo Fisher Scientific) with a preceding murine IgG1 signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... Wild type/native human basigin (BSG-2, residues M1-L206) was expressed in Expi293™ cells (Thermo Fisher Scientific) with C-terminal rat CD4 domains 3+4 (CD4d3+4 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human Alexa-647 [Cat# A21445] and streptavidin-AlexaFluor 635 [Cat# SA1011] antibodies were obtained from Thermo Fisher. Control lectins Erythrina cristagalli agglutinin (ECA ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed once with PBS and incubated with 1 ug/ml anti-human (Alexa Fluor 647; Invitrogen) secondary Abs and the viability dye AquaVivid (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Human foreskin fibroblasts (HS27, ATCC CRL-1634) were hold in culture in Iscove’s Modified Dulbecco’s Medium (IMDM, Invitrogen/Gibco) with the same supplementations ...
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bioRxiv - Neuroscience 2020Quote: 100 ng of total RNA was subjected to hybridization on the Human Clarion S Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Neuroscience 2020Quote: 100 ng of total RNA was subjected to hybridization on the Human Clarion S Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Cell Biology 2021Quote: Young adult primary human skeletal muscle (HSKM) progenitors isolated from a 20-year old female subject’s quadriceps muscles (Gibco) were seeded onto plates coated with 0.1% gelatin solution (Merck-Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of Geltrex (Gibco, A14133-01), in Essential 8 Basal Medium (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... We used summarized log-scale intensities representing copy number profiles generated by combining probe intensities from four platforms (Agilent Human Genome CGH Microarray 44A, Nimblegen HG19 CGH 385K WG Tiling v2.0, Affymetrix GeneChip Human Mapping 500k Array Set and Illumina Human1Mv1_C BeadChip) ...
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bioRxiv - Cancer Biology 2019Quote: ... The HER4 JMa/CYT1 human sequence was amplified by PCR and subcloned in the pcDNA3.1 vector (Invitrogen, Carlsbad, CA) to create pCMV_HER4 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were further diluted 1:100 in standard diluent and Aβ levels were measured with the human Aβ1–42 ELISA kit (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: Silencing lentiviral vectors were produced by co-transfecting HEK293T producing cells with lentiviral silencing plasmids GIPZ Human histamine H3 receptor shRNA (Clone V3LHS_638095 or Clone V3LHS_638091, Thermo Scientific) with packing plasmid psPAX2 and envelope coding plasmid pMD2.G (Addgene#12260 and #12259 ...
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bioRxiv - Biochemistry 2021Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (hTERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... The microarray slides were probed with Fc-tagged DC-SIGN and L-SIGN at 10 μg/ml diluted in 1% BSA (TSM with 0.05% Tween) and binding was detected with Alexa Fluor 488 labelled goat anti-human IgG (H+L) (Invitrogen) at 5 μg/ml.
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bioRxiv - Biochemistry 2020Quote: ... and the HsGlud2 cDNA clone (NM_012084.3) was obtained from the Ultimate™ ORF Lite human cDNA collection (Life Technologies). Amino acid mutations were introduced via Quick Change site-directed mutagenesis as previously described(58) ...
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bioRxiv - Bioengineering 2021Quote: ... Secreted cytokine analysis was then performed using a ProcartaPlex essential Th1/Th2 human cytokine immunoplex assay according to manufacturer’s instructions (Invitrogen). Immunoassay was then read on a Luminex 200 instrument controlled by BioPlex manager software (version 6.2).
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells, ATCC CCL-244) were maintained in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... USA) and Huh7.5 (human liver cell line) (kind gift from Dr. Craig Cameron) were cultured in DMEM (Life Technologies) containing 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Genetics 2021Quote: ... A database search was performed using the human SwisProt sequence and Orf8 sequence with Proteome Discoverer 2.4 (Thermo Scientific) with the following search criteria ...