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Citations for Thermo Fisher :
3801 - 3850 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Immortalized human umbilical vein endothelial cells (iHUVEC, gift from Dr. W. Muller, Northwestern University [NU]) were cultured in human endothelial serum-free medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with PBS and stained with 2 μg/ml goat anti-human (Alexa Fluor 647; Invitrogen) secondary Abs for 15 min in PBS ...
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bioRxiv - Genetics 2019Quote: ... Extracted DNA samples were quantified following the Quantifiler Duo Human DNA Quantification Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) as recommended by the manufacturer in 7500 Real-time PCR System (Thermo Fisher Scientific Company ...
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bioRxiv - Cell Biology 2021Quote: ... of the MCF7 human ductal breast carcinoma cell line[64](hereafter referred to as MCF7) was cultured in RPMI medium (Gibco) supplemented with 6% FCS and in a humidified incubator set to 37°C and 5% CO2 ...
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bioRxiv - Genomics 2021Quote: Human osteosarcoma (U2OS, ATCC HTB-96) cell lines were maintained in McCoy’s 5A Modified Medium (16600082, Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were activated for two days with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher Scientific, Waltham, MA). After two days ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
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bioRxiv - Bioengineering 2021Quote: ... 105 PBMCs derived from a healthy human donor were stimulated with T cell activating beads (anti-CD3/CD28 Dynabeads, ThermoFisher) at a 1:1 PBMC:bead ratio in each well containing MSCs ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human astrocytes were grown on the bottom of transwell inserts (24-well, 0.1 um pore size, PET, Fisher Scientific) which were coated with poly-L-lysine (20 ug/mL) ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized human SERT was assembled from synthetic DNA fragments (IDT) and inserted into the NheI-XhoI sites of pCEP4 (Invitrogen). The use of a synthetic gene ensures there are no sequence features ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were generated by RUCDR Infinite Biologics® from human primary lymphocytes using Sendai viral vectors (CytoTune™, ThermoFisher Scientific), as previously described (Moore JC et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was produced in 293T cells and human melanoma cell lines were infected and selected with 1 μg/mL Puromycin (Gibco) for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: Human foetuses were obtained following elective termination of pregnancy with full consent and stored overnight in Hibernate-A Medium (Gibco) at 4° C ...
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bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Cell Biology 2021Quote: ... A probe mix containing 60 ng of each library probe and fluorescently labeled readout probe and 40 μg human COT1 DNA (Invitrogen) in 1.1 ml of hybridization buffer (20% dextran sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 cells were stained with NCAM-1 antibody (CD56 Anti human Alexafluor 700, 1:200 dilution, Fisher Scientific, BDB557919) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... NOK1 cells were grown in keratinocyte-SFM medium supplemented with human EGF and bovine pituitary extract (BPE) (Thermo Fisher Scientific) and induced to differentiate by transfer to Dulbcecco modified Eagle medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... human GRPR shRNA in Lentiviral Vector (Genomics Online; Limerick, PA, # ABIN3479889) and mouse Grpr siRNAs (Life Technologies, #157912 and #157914) were used for all knockdown experiments ...
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bioRxiv - Cell Biology 2021Quote: Human non-transformed retinal pigment epithelial cells transduced with the human telomerase gene (hTERT-RPE1; ATCC CRL-4000) were grown in DMEM/F12 (Gibco) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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bioRxiv - Bioengineering 2020Quote: ... 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen), FITC-conjugated anti-human CD4 (BD Bioscience) ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Cell Biology 2021Quote: Human islet and hESC-derived differentiated organoids (approximately 20 organoids or human islets per well) were respectively plated into a 96-well black plate (ThermoFisher) coated with 1% hESC-qualified Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... human serum or oseltamivir were serially two-fold diluted in Dulbecco’s phosphate buffered saline with calcium and magnesium (ThermoFisher Scientific) containing 1% BSA (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... ATCC) and HeLa (a human cervical cancer cell line, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from human cells (1 x 106 cells) using the TRIZOL reagent according to the manufacturer’s instructions (ThermoFisher Scientific). RNA integrity for each sample was confirmed with by Nano Drop ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Immunology 2020Quote: ... and human fibroblasts (HF) clone EF-1-F (sourced from Doorbar lab, University of Cambridge) were grown in MEM (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500ng of a subset of ACE2 expression constructs (human, hamster, rabbit, pig, chicken, horseshoe bat) or mock transfected with an empty vector (pDISPLAY) in OptiMEM (Gibco) using TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... Caco-2 (human epithelial colorectal adenocarcinoma, kindly provided by Prof. Holger Barth, Ulm University) cells were cultivated in DMEM (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM with 1 μg/mL of puromycin (Invitrogen). TMPRSS2 human plasmid (Origene ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/mL of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells ...