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Citations for Thermo Fisher :
3651 - 3700 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were co-transfected with the R278C-sgRNA-pCCCplasmid and the ssODN donor template using Lipofectamine 3000 (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Human microglial cells were cultured in cell culture flasks coated with 50 μg/ml rat tail collagen I (Gibco) in high glucose DMEM (4.5 g/l ...
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bioRxiv - Cell Biology 2023Quote: ... custom micropatterned glass coverslips presenting 500 µm groWTh areas (CYTOOCHIPS Arena 500A) were covered with 800µL of 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were grown in glass surfaces previously coated with 10μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC lines were generated from human peripheral blood CD34+ hematopoietic stem cells (HSC) with a CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The human Rhabdomyosarcoma muscle cell line (RD, ATCC CCL-136) was maintained in Dulbecco’s Modified Eagles Medium (DMEM, Gibco) containing 10% FBS and 1% P/S ...
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bioRxiv - Immunology 2023Quote: ... The human embryonic kidney 293T/17cells (ATCC, CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (Gibco Life Technologies) containing 10%FCS ...
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bioRxiv - Systems Biology 2023Quote: ... Thawed Primary human hepatocytes were recovered in Cryopreserved Hepatocyte Recovery Medium (Cat. CM700, Thermo Fisher Scientific, Waltham, MA, USA), and re-suspended in ice-cold HBSS prior to surgery ...
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bioRxiv - Immunology 2023Quote: CHO cells that were stably transfected with human CTLA-4 have been reported.23 CHO cells were grown in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... ATCC CCL-81) and human embryonic kidney cells (HEK293T; ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The human erythroleukemia cell line TF-1 (ATCC, USA) were cultured in RPMI-1640 media (Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum and activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) at a 1:1 bead:cell ratio according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco) at 1:8 beads:T cell ratio ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human neutrophils were used directly out of thaw and cultured on poly-l-lysine (15 µg/mL, Thermofisher) coated tissue culture dishes ...
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bioRxiv - Cancer Biology 2023Quote: ... and for human biopsies (see Fig. S1B and C) goat anti-ZO-1 (Thermo Fisher; PA5-19090; 1:200) and anti-laminin primary antibodies were used ...
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bioRxiv - Bioengineering 2023Quote: ... Two different lots (lot 1355434 and 1767629) of human dermal neonatal foreskin fibroblasts (hDFn) were purchased from Thermo Fisher Scientific (cat ...
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bioRxiv - Bioengineering 2022Quote: Human osteosarcoma U2OS cell lines (HTB-96, ATCC, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, GIBCO, USA) containing 10% heat- inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Enriched neutrophils were resuspended at 1×106 cells/mL in RPMI with 20 ng/mL GM-CSF (R&D or STEMCELL) (for murine neutrophils) or RPMI with 2% FBS (for human neutrophils) with 1μM Sytox Green (Invitrogen) and plated in 96-well plates at 200,000 neutrophils per well ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession: BC140778.1) and subcloned into a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag ...
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bioRxiv - Cell Biology 2022Quote: ... Endometrial ADGR gene expression in these 21 samples was determined using the high-density human genome (HG) U133 Plus 2.0 Arrays (Affymetrix), containing 42,203 genes and 12,397 expressed sequence tags (ESTs) ...
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bioRxiv - Cancer Biology 2023Quote: Human embryonic kidney 293T (HEK293T) cells were purchased from ATCC and cultured in DMEM media (Thermo Fisher, 11965-092) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cancer Biology 2023Quote: ... The human GCB-DLBCL cell line SU-DHL4 (RRID:CVCL_0539) was obtained from DSMZ and grown in RPMI 1640 (ThermoFisher; 11875093) with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...
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bioRxiv - Genetics 2022Quote: ... and human CD45+ (hCD45+) bone marrow (BM) cells from transplanted mice using the PureLink Genomic DNA Mini Kit (Invitrogen). DNA was digested using DraI restriction enzyme (NEB ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2023Quote: ... and HEK293T (human embryonic kidney cells, ATCC CRL-3216) cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Each pool was labeled and hybridized to Affymetrix® Human Gene 2.1 ST according to the manufacturer instructions (Affymetrix). Quality control and normalization of Affymetrix.CEL files using the Robust Multi-array Average (RMA ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Genomics 2023Quote: ... falciparum clone II313 was maintained in human A+ erythrocytes at 37 °C in RPMI 1640 medium containing AlbumaxII (Invitrogen) supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or ALK1 (SMARTpool ON-TARGETplus Human ACVRL1 siRNA, Dharmacon L-005302-02-0005) using Lipofectamine 3000 (ThermoFisher L3000015) according to manufacturer directions ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney 293T cells (HEK293T) and Vero E6 cells were maintained in Dulbecco modified Eagle’s medium (DMEM; Gibco) with 8% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were washed with 1% human albumin in PBS and stained with propidium iodide (Molecular Probes #P3566) for assessing viability ...
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bioRxiv - Cell Biology 2023Quote: ... The digested tissue was mixed with an equal volume of 1% human albumin (CSL Behring) in DPBS −/− (Gibco #14190094) to stop the lysis ...
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bioRxiv - Neuroscience 2023Quote: ... engraftment was measured weekly by quantifying human CD45+ cells in each mouse’s peripheral blood through fluorescence-activated cell sorting (FACS) on Attune flow cytometer (ThermoFisher). On average ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cat#40590-D001) and anti-human AF488 (1:1000 with final concentration of 2μg/ml; ThermoFisher Cat#A-11013) for secondary conjugation ...
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bioRxiv - Immunology 2023Quote: Human NK cells were seeded at a density of 2×106 cells/mL in a 24-well plate (NUNC) in the presence of IL-15 (10 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St. Louis, MO, USA or Thermo Fisher Scientific, Waltham, MA, USA), 2% human plasma (isolated from umbilical cord blood provided by Stanford Binns Cord Blood Program) ...