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Citations for Thermo Fisher :
3351 - 3400 of 5182 citations for Endostatin Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The human monocytic THP-1 cell line (from ATCC) was cultured in RPMI1640 medium (Thermo Fisher Scientific Inc.) supplemented with 10% FBS and stimulated with OxLDL1 and OxLDL2 at a concentration of 20 µg/mL.
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bioRxiv - Immunology 2024Quote: NK92 cells expressing human FcγRIIIa (CD16.NK-92) (ATCC) were maintained in α-MEM without nucleosides (Thermo Fisher), 2mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney HEK293T (ATCC, catalog #: CRL-3216) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK)-293T cells (ATCC CRL-3216) were cultured in 10% FBS DMEM (Gibco 11965-092), supplemented with penicillin (50 U per mL ...
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bioRxiv - Immunology 2024Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Immunology 2024Quote: ... Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco, A29133). IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: Human cervical adenocarcinoma cells (HeLa) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with GlutaMAX™ (Gibco, ThermoFisher) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... plated at 80,000 cells/chamber and chemoattracted to growth medium containing 50 nM of human HGF (Life Technologies). Drugs were added in transwell assays at the time of cell seeding ...
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bioRxiv - Microbiology 2024Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Gibco, 12800082) supplemented with 10% FBS (Pan Biotech ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Gibco, 12800082) supplemented with 10% FBS (Pan Biotech ...
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bioRxiv - Cancer Biology 2024Quote: Primary Human Hepatocyte: Cytosolic and nuclear fractions were prepared using the NE-PER cell fractionation kit (Thermo Fisher) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with Alexa Fluor 647-conjugated goat anti-human antibody (Invitrogen; cat# A-21445, 1/500). The control VHH Fc (R3VQFc ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney 293T cells (HEK293T) 50 were maintained in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in a 1:1:1:1 ratio of human receptor:Gαq-Rluc8:β3:GFP2-γ9 DNA constructs prepared in Opti-MEM (Invitrogen) using a 3:1 ratio of TransIT-2020 (Mirus Bio ...
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bioRxiv - Neuroscience 2024Quote: Human Embryonic Kidney 293T (HEK293T) and HeLa cells were cultured in DMEM supplemented with 10% FBS (Life Technologies). 1 % trypsin was employed to detach cells and split them ...
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bioRxiv - Immunology 2024Quote: ... incubated for 1h with either horseradish peroxidase (HRP)-conjugated goat anti-human IgM or IgG (Invitrogen, Camarillo, CA), washed again ...
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bioRxiv - Biophysics 2024Quote: Human cervical adenocarcinoma cells (HeLa) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with GlutaMAX™ (Gibco, ThermoFisher) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Systems Biology 2024Quote: 150 human islets were hand-picked on the day of distribution and placed in 1ml of Trizol (Invitrogen), vortexed and stored at −80°C until shipping to Oxford or Stanford for further processing ...
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bioRxiv - Microbiology 2024Quote: ... and anti-human IgG conjugated to Alexa 488 and Alexa 555 were purchased from Invitrogen (Thermo Fisher Scientific). The Golgi (RCAS1 ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Telomerase-immortalized human myometrial (hTERT-HM) cells (52) were cultured in phenol-red free DMEM/F12 (ThermoFisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... human and primary mouse myotubes were maintained in a mixture of DMEM/F-12 (Gibco: Waltham, MA, USA) containing 20% FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401). Human iPSCs from the GIH6C1 background were cultured in mTeSR Plus medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: Plasmodium falciparum parasites were grown in human erythrocytes (Stanford Blood Center) at 2% hematocrit in RPMI 1640 media (Gibco) supplemented with 0.25% Albumax II (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: The HaCaT cell line (Human adult low Calcium high Temperature) were grown in Dulbecco’s Modified Eagle Media (DMEM, Invitrogen) supplemented with 2 mM essential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Genetics 2021Quote: ... Fetal cortex samples were acquired from the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) (http://www.hdbr.org/) in Hibernate-E media (ThermoFisher Scientific). All samples were obtained through elective terminations of pregnancy ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by performing a Ficoll-Paque gradient (Thermo Fisher) on whole blood ...
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Immunology 2020Quote: ... Samples were thawed on ice and prepared according to the manufacturer’s instructions using a custom-made 33-plex human cytokine panel (Procartaplex ThermoFisher). In brief ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: The screening was performed with a genome-wide siRNA library (Ambion SilencerR Human Genome siRNA library v3, Thermo Fisher), composed of siRNAs targeting approximately 21000 genes arrayed on 384-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... kidney) and HEK293T (Human embryonic kidney) cell lines were cultivated in Dulbecco’s modified Eagle (DMEM) (Thermo Scientific #12100-046) medium ...
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bioRxiv - Microbiology 2021Quote: Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were cultured in keratinocyte serum-free medium (KSFM) (Gibco) with 0.5 ng/mL human recombinant epidermal growth factor and 0.05 mg/mL bovine pituitary extract ...
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bioRxiv - Neuroscience 2022Quote: Human iPSCs (male WTC11 background, PMID 24509632) were cultured in StemFlex™ Basal Medium (Gibco; Cat. No. A33493-01) on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Human female osteosarcoma U2OS and breast cancer MCF7 cells were cultured in DMEM + Glutamax (Thermo Fisher Scientific, 31966-047) supplemented with 10% FCS and 1% Penicillin/Streptomycin ...
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bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA expression analysis was performed with Affymetrix Human ClariomS® microarray using the WT Plus Reagent kit (ThermoFisher Scientific). As starting material ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... mouse fibroblast NIH-3T3 L929 cells and human embryonic kidney HEK 293 cells were grown in DMEM medium (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with sterile-filtered 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... Human WT (GM03652), NPC1 (GM03123), and NPC2 (GM18455) fibroblasts (Coriell Institute, Camden, NJ) were maintained in DMEM media (Invitrogen) supplemented with 15% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
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bioRxiv - Microbiology 2019Quote: ... and C were selected and designed using MEGA6 software [30] and evaluated by the Thermofisher custom genomic TaqMan assay and design pipeline for non-human templates (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping) and analyzed using the ABIprism 7900HT (Applied Biosystems): SpaP SNP2137-2139 [GT/CG] ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).