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Citations for Thermo Fisher :
3451 - 3500 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed twice with cold FACS buffer (PBS + 2% FCS) and incubated in the dark at 4° C with an anti-TfR1 (CD71) antibody (clone OKT9; Thermo Fisher Scientific, Waltham, MA, USA). Cells were then washed with PBS and resuspended in FACS buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The suspended material was filtered through a 76 μm sterile filter and subsequently cultured in DMEM/F12 phenol red-free medium supplemented with 10% heat-inactivated fetal calf serum (FCS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin (C-medium) ...
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bioRxiv - Developmental Biology 2020Quote: 30,000 thawed hESCs were cultured in single wells of a 48-wells plate and decidualized by adding DMEM/F12 phenol red-free medium supplemented with 10% heat-inactivated charcoal stripped FCS (Thermo Fisher Scientific, Waltham, MA, USA), 0.5 mM 8-bromoadenosine 3□,5□-cyclic monophosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured and maintained at 37 °C in a humidified atmosphere of 5 % CO2 in air in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutMAXTM-1 containing 10 % Fetal calf serum (FCS) and 1% Penicillin-Streptomycin (10 000 U/ml) (Gibco Life Technologies, Grand Island, NY, USA). The RPE-1 cell line was cultured with 50 %DMEM/50% F10 Nutrient Medium supplemented with 10 % FBS ...
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bioRxiv - Physiology 2022Quote: ... henceforth referred to as ‘supplemented a-MEMS,’ comprised α-MEMS media supplemented with 10% FCS and 1% penicillin/streptomycin (all Life Technologies, Carlsbad, CA, US). On the day of peptide addition to the cells ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific, Loughborough, UK). All cell types were cultured at 5% carbon dioxide (CO2 ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: 3T3-F442A preadipocytes were grown and differentiated in a modified DMEM designed for isotope labeling in cell culture [Sigma Aldrich (Saint Louis, MO, USA)] supplemented with 10% dialyzed FCS (GIBCO-Thermo Fisher Scientific, Eugene, OR, USA), 125mg/mL streptomycin ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The change in absorbance at 340 nm due to oxidation of NADH was recorded in a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA). The data were fitted to the Michaelis-Menten equation to obtain values for the actin concentration at half-maximal activation of ATP-turnover (Kapp ...
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bioRxiv - Biochemistry 2021Quote: ... All RNA solutions were prepared by solubilizing in nuclease free water in the presence of RNAseOUT™ recombinant ribonuclease inhibitor (Thermo Fisher, Cat. #. 10777019) at a final concentration of 0.4 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then co-transfected with recombinant plasmid (300 ng) together with miR-544-3p mimic or negative control mimic (50 nM) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) and Lipofectamine RNAimax (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... DMEM High-glucose [Gibco] without Riboflavin and Phenolred containing 10% [w/w] FCS [Gibco], 1% [w/w] P/S [Gibco] and 1 % [w/w] Glutamax [Gibco]) supplemented with 1 μM TO-PRO®-3 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Then cells were washed 3 times with FC buffer followed by staining with goat α-rabbit IgG Alexa-568 (Molecular Probes Cat# A-11011, RRID: AB_143157); for 45 min in FC buffer to analyse the GFP percentage corresponding to virus infection on transfected cells ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA, Merelbeke, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The VHH M13 phage-display library was subjected to two rounds of affinity enrichment (panning) against immobilized recombinant OspA on Nunc-Immunotubes™ (Thermo Fisher Scientific, Waltham, MA) using protocols described elsewhere (56) ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Molecular Biology 2023Quote: ... this latter was performed on 1 ng of genomic DNA (gDNA) of each organism listen in Table 2 by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Pathology 2022Quote: Cells isolated as described in “Erythrocytic Compartment Preparation” and “Tissue Leukocyte Isolation” were incubated with Fc block (1:100) and LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (ThermoFisher Scientific, #L34959; 1:5000 in PBS)(10min. ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).