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Citations for Thermo Fisher :
3301 - 3350 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
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bioRxiv - Immunology 2022Quote: ... and suspended in Fc Block for 5 min at RT prior to staining with a surface stain of following antibodies: CD3 (Invitrogen, 45-0031-82)/CD4 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of glucose in filtrate was quantified using Glucose CII-Test Wako (FUJIFILM Wako Pure Chemical Corporation) by measuring the absorbance at 492 nm with a Thermo Scientific Multiscan® FC (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 340 nm was read every 12 s at 37°C using a Multiskan FC plate-reader (Thermo Scientific, Paisley, UK). Maximum absorbance ...
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bioRxiv - Microbiology 2020Quote: ... Then the resultant supernatants were transferred to a 96-well plate for the absorbance measurement at 492 nm with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA). RBCs treated with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Planktonic cell growth was determined by quantifying optical densities at 595 nm using a microplate spectrophotometer (Thermo scientific multiscan FC: Cat no. 51119000). Plates were thoroughly washed three times with 200 µl of sterile PBS per well using a microplate washer (Micro wash 1100 from Global diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended at a concentration of 1 × 106 cells/mL in RPMI without FCS and labeled with CellTracker™ Blue CMAC dye (10 μM, Thermo Fisher, C2110) for 20 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 cells were maintained in DMEM media supplemented with FCS (10 % final concentration) and Penicillin Streptavidin-Glutamine (PSG; Gibco, 1 % final concentration). For starvation ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Molecular Biology 2021Quote: ... using DMEM (4.5 g/l glucose) with 2 mM Glutamax-I medium supplemented with 15% foetal calf serum (FCS) (ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Genomics 2024Quote: ... tissue-culture plates in DMEM (4.5 g/L glucose) with GlutaMAX-I supplemented with ES-tested 15% foetal calf serum (FCS, ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The absorbance reading was conducted at 595 nm using a microplate photometer (IVD model, MultiskanTM FC, Thermo Fisher Scientific Inc., MA, United States).
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bioRxiv - Systems Biology 2023Quote: Murine 3T3-L1 preadipocytes differentiated into mature adipocytes by first incubating confluent cells in induction-medium (DMEM GlutaMAX™, Gibco®, 10% FCS) containing 1.6μM insulin ...
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bioRxiv - Microbiology 2023Quote: ... Hungary) substrate and the optical density of the solution was measured at 450 nm using a Multiscan FC reader (Thermo Fisher Scientific Inc.). Blood samples were centrifuged after collection and the sera were kept at −70°C ...
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bioRxiv - Biophysics 2023Quote: ... setup alignment and quantifying confocal volume parameters were performed with fluorescence correlation spectroscopy (FCS) using ATTO425-COOH, ATTO488-COOH, Alexa Fluor 546 or ATTO655-COOH (ATTO-TEC GmbH, Siegen, Germany) (Alexa Fluor™, Thermofisher Scientific). Data was analyzed using PAM-FCSfit (Supplemental Figure S5A) ...
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bioRxiv - Biochemistry 2023Quote: ... Phage pools representing the libraries were cycled through rounds of binding selections with EPHA2-FN2-Fc fusion protein immobilised on Maxisorp Immuno plates (ThermoFisher, #12-565-135), as described previously [21] ...
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bioRxiv - Cell Biology 2023Quote: ... using either a WPA CO8000 Cell Density Meter (Biochrom, Cambridge, UK) or a Multiskan FC microplate reader with a 594 nm filter (Thermofisher, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Proteins were then quantified with Image Studio Version 5.2.4 (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The single cell suspension was incubated with 1:1000 Fc Block (AbLab) in 1X FACS Buffer and stained for surface markers before being fixed/permeabilized (Thermofisher 00-5523-00) for FoxP3 staining (invitrogen 11-5773-82 Table 2).
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 50nM Aβ-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... After lysis of red blood cells with Gibco ACK lysing buffer and blocking of Fc receptors (Invitrogen CD16/CD32 clone 93, RRID AB_467133), cells were stained with live/dead aqua (Thermo ...
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bioRxiv - Microbiology 2024Quote: ... The isolated cells were resuspended in DMEM supplemented with 5% FCS and immediately plated on glass coverslips using Cytofunnels (Thermo Fisher Scientific, USA) and centrifugation at 1500g for 10 minutes with a Cytospin 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... IPS cells were thawed and cultured at all times cultured on recombinant human laminin LN521 (Biolamina, 2021-21) kept in E8 basal medium (Thermo Fisher Scientific) supplemented with primocin (0.1 μg/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus carrying hXkr8-TH-mGFP or hBSGΔ cDNA was obtained with the Bac-to-Bac Baculovirus Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
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bioRxiv - Biochemistry 2022Quote: ... Rhodopsin expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...