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Citations for Thermo Fisher :
3151 - 3200 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Optical density of standards and samples were measured at OD595 nm using a Multiskan™ FC Microplate Absorbance Reader (Thermo Scientific, Belgium). Plasma and cell lysate were prepared by mixing 100 µl citrated plasma with 100 µl RIPA buffer and 6 × 106 HUVECs with 250 µl RIPA ...
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bioRxiv - Microbiology 2022Quote: Expression plasmids encoding CLII or CLIV with an Fc- and His6-tag were transfected in Expi293F expression cells (Gibco-Thermo Fischer Scientific) using the ExpiFectamine293 transfection kit ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation (500 x g, 5 min) and washed with CO2-independent medium supplemented with 10 % FCS (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C with 5% CO2 and transfected by different expression constructs for human IgG-Fc with various signal peptide coding regions using LipofectAmine (Invitrogen, Calsbad, CA) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: NIH3T3 and HEK293FT cells were cultured under standard conditions (DMEM; 10% FCS, 1% penicillin/streptomycin, all from Invitrogen; 37°C; 5% CO2).
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bioRxiv - Immunology 2021Quote: ... immune cells in the 70-30% interphase were collected and washed twice with RP10 (RPMI 1640, Thermo Fisher; 10% FCS, 1% penicillin/streptomycin (Invitrogen, #15140122) 1% glutamine (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... NKG2DL expression was assessed by staining with NKG2D-Fc chimera (10 µg / mL; Fisher 1299NK050) followed by an αFc secondary stain (Invitrogen #A-10631). NIR Live/Dead (ThermoFisher #L34976) ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Cell Biology 2021Quote: ... and A9 cells (obtained from Nobuo Takagi at Hokkaido University in 1980s) were cultured in DMEM (Nacalai Tesque) with 10% FCS (Thermo Fisher Scientific) and 1% GPS solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with heat-inactivated 10% v/v foetal calf serum (FCS; HyClone) and 500 μg/mL Geneticin (Gibco cat no 10131-0275) and kept under 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2020Quote: BM cells of C57Bl/6 donor mice were collected by flushing tibias and femurs by using 10 mL syringes (Terumo) filled with cell culture medium (DMEM, 10% FCS, 1% Penicillin-Streptomycin, 1% Antibiotic-Antimycotic, Thermo Fisher Scientific) (Kruger et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The absorbance at a wavelength of 450 nm was measured using Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA USA).
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: Whole lung single cell suspensions were passed through a 40 µm cell strainer and preincubated with anti-mouse CD16/CD32 Fc block (1:100, Thermo Fisher Scientific) for 15 min in flow buffer (PBS supplemented with 5% (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... Sendai virus-infected primary fibroblasts were immediately centrifuged for 45 min at 32 °C with 1,500 g (spinfection) and cultivated in Advanced DMEM containing 5% fetal calf serum (FCS) and 1% GlutaMAX™ (all from Life Technologies). On the following day the viruscontaining medium was replaced with fresh culture medium ...
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bioRxiv - Bioengineering 2021Quote: ... Recovered nanovials were washed two times with washing buffer and sequentially labeled with streptavidin and biotin goat anti-human IgG Fc (Thermo Fisher, A18821) following standard procedures described above ...
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at a density of 2.7×104 cells/cm2 and cultured in William’s Medium E (Biochrom, Berlin, Germany) containing 10% (v/v) FCS (Life Technologies, Darmstadt, Germany), 5 mg/ml insulin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
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bioRxiv - Cell Biology 2020Quote: ... NIH3T3 and RPE-1 were maintained in DMEM (HPSTA – high glucose, stable glutamine and sodium pyruvate; Capricorn Scientific) supplemented with 10% fetal calf serum (FCS; Thermo Fisher Scientific) under standard conditions at 37°C in a 5% CO2 environment ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed with PBS and blocked with R10 media (RPMI-1640 with 10% FCS, 1% Pen/Strep, 2mM L-Glutamine (all from Thermo Fisher Scientific)) for 30 minutes at RT before the addition of freshly isolated PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... OD595 was measured every 5 min at 37°C from cultures that underwent continuous shaking in a Multiskan™ FC Microplate Photometer (Thermo Scientific). Data were collected from at least two independent experiments with at least two replicates for each individual experimental condition.
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Microbiology 2021Quote: ... Two photometric readings were conducted for each plate with a 540 nm filter on a Multiskan FC Microplate Photometer (Thermo Scientific 51119000). The protocol is a slightly modified version of an Enzymatic Assay of α-Amylase (https://www.sigmaaldrich.com/NL/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of 0.2 mM sulfuric acid was added to halt the enzymatic reactions and the optical densities (OD) of the contents in the wells were read at 450 nm using a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Cell Biology 2022Quote: ... the absorbance of each well at 450 nm was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (BioConcept, Cat# 2-01F16-I) and 100 units/ml of penicillin and 100 µg/ml of streptomycin (Invitrogen, Cat# 15140122). Rat primary fibroblasts (REFs ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies, Life Technologies, Carlsbad, CA, US). 3T3-FB were incubated with MNP (E-SO-2-MNP ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies, Life Technologies, Carlsbad, CA, US). 3T3-FB were incubated with MNP (E-SO-2-MNP ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100 µL FACS buffer supplemented with Fc-γ receptor anti-CD16/CD32 antibodies (1:100, Thermo Fisher Scientific) and incubated on ice at 4°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in complete medium (RPMI 1640 supplemented with 10% fetal calf serum, FCS, 100 U/mL penicillin and 100 μg/mL streptomycin; Sigma-Aldrich and Invitrogen respectively).
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bioRxiv - Microbiology 2024Quote: ... 250 μL of each sample was transferred into individual wells of a clear bottomed 96-well plate and the absorbance read at 620 nm (Multiskan FC, Thermo Fisher Scientific). Experimental glycogen concentrations were calculated off the standard curve (Herrmann & Gehringer ...
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bioRxiv - Microbiology 2024Quote: ... The plate was incubated at room temperature for 10 min after which the absorbance at 595 nm was determined (Multiskan FC, Thermo Fisher Scientific). Experimental protein concentrations were read off the standard curve (Herrmann & Gehringer ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... The cells were selected for three passages in DMEM supplemented with 10% FCS and 500 μg/mL G418 sulfate (ThermoFisher Scientific, 10131027). The extent of overexpression was assessed with flow cytometry (Suppl ...
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bioRxiv - Immunology 2023Quote: ... Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076] supplemented with 2% FCS and 100x Penicillin-Streptomycin [Gibco, #10378-016]).
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bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
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bioRxiv - Cancer Biology 2023Quote: ... Uveal melanoma cell lines were maintained in RPMI and cutaneous melanoma cells and HeLa cells were cultured in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
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bioRxiv - Genomics 2023Quote: Lymphoblastoid cell lines (LCLs) from healthy donors and ICF2 patients were cultured in RPMI 1640 supplemented with 15% FCS and antibiotics (Thermo Fisher Scientific) as described in (15).
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...