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Citations for Thermo Fisher :
3001 - 3050 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Inserts were gently washed and placed in serum free DMEM and the upper chamber filled with DMEM supplemented with 10 % FCS (Life Technologies) and either PBS or 100ng/ml FGF10 (Peprotech) ...
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Molecular Biology 2019Quote: ... female) cells and C2C12 (ATCC® CRL-1772™) cells were maintained in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in RPMI 1640 medium containing 10% FCS was labelled with 10μM CFSE dye (Thermo Fisher Scientific, catalogue# C34570) for 4 minutes at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were resuspended at a concentration of 2.5-5 × 106 cell/mL in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (F14201, Thermo Fisher Scientific) and 3 μM Fura Red (F3021 ...
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bioRxiv - Immunology 2020Quote: ... Absorbance was measured at 450 nm with a reference wavelength of 620 nm using the Multiskan FC Microplate Photometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2020Quote: ... cells were adjusted to 10.106 -20.106 /mL in FACS buffer (PBS, 1% FCS, 5% Hepes, 0.1% Azide) containing SYTOXTM Orange Dead Cell Stain (recommended dilution, Thermo Fisher Scientific) for nuclear staining ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were mechanically homogenised into single cell suspensions in RF10 media (RPMI 1640, 10% FCS, 1× penicillin-streptomycin-glutamine; Life Technologies). Isolated cells were stained with Aqua viability dye (Thermofisher ...
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bioRxiv - Genomics 2021Quote: ... Live cells were identified by washing after Fc block and incubation with Fixable Viability Dye Violet (Invitrogen, cat. L34955, 1:1000) prior to surface staining or incubation with calcein blue (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Neuroscience 2022Quote: ... SH-SY5Y cells were replated into primary astrocytes in 6-well plates in density 1.103cells/cm2and co-cultivated for further 5 days in DMEM medium (PAA Laboratories GmbH, Germany) containing 1% FCS (Invitrogen, Carlsbad, CA), 2 mM L-glutamine (PAA Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellets were resuspended in 100 μL of FC buffer containing LIVE/DEAD violet dye (Thermo Fisher, 1:1,000 dilution) and secondary antibodies (goat anti-mouse IgG Alexa 647 conjugated ...
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bioRxiv - Physiology 2024Quote: ... The optical density of the reaction product was read at 450 nm using the Multiskan FC microplate reader (Thermo Scientific, USA) after the initial incubation period (3 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...
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bioRxiv - Neuroscience 2024Quote: ... substituted with 10% fetal calf serum (FCS; Capricorn Scientific, Palo Alto, CA, USA) and 2 mM L-glutamine (Invitrogen, Carlsbad, USA), 50 U/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The level of CV was quantified by measuring the absorbance at 540 nm with a MultiskanTM FC Microplate Photometer (Thermo Scientific). The average value from the blank (acetic acid only ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were stained directly using a nanobody against ALFA tag coupled to a Abberior Star 635P fluorophore (FluoTag®-X2 anti-ALFA - AbberiorStar635P, NanoTag Biotechnologies GmbH) diluted 1: 500 in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 250µg/ml in culture media (RPMI 1640 supplemented with 10 % FCS, 55 µM β-mercaptoethanol and Penicillin-Streptomycin-Glutamin 100x (Gibco)) for 2h at 37°C and washed twice ...
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bioRxiv - Developmental Biology 2024Quote: ... manually minced and enzymatically digested in an orbital shaker for 2 hours at 37°C in digestion mix (9.2 ml DMEM/F12, 5% FCS, 1% Penicillin/Streptomycin, 25 mM HEPES (Gibco, cat. #15630056) and 300 U/ml Collagenase IV (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... after which cells were stained for 1 hour at room temperature with AlexaFluor-conjugated secondary antibodies diluted in PBS+10% FCS (Life technologies) and stained with DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted CD31-CD45-CD184+ cells were cultured in complete growth medium DMEM supplemented with 20% FCS and 1% penicillin/streptomycin (Gibco, 15140122).
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bioRxiv - Immunology 2023Quote: ... in PBS for 10 min prior to addition of the relevant surface marker fluorochrome-conjugated antibodies in FACS buffer (PBS, 2% heat-inactivated FCS) supplemented with Super Bright staining buffer (Thermo Fisher). Following 30 min of incubation ...
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bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
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bioRxiv - Immunology 2023Quote: ... were performed according to the manufacturer’s instructions with 50 000 cells per well and read at 492nm on a microplate absorbance reader (Multiskan FC microplate photometer, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Purified Fc-FGF-21CT was subject to an endotoxin removal proces (Pierce High Capacity Endotoxin Removal Spin Columns, Thermo Fisher Scientific) before flash-frozen and stored at -80°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD-CD45-CD235A-CD71- non-hematopoietic fraction was sorted in DMEM supplemented with 10% FCS for Single-cell RNA sequencing and 7AAD-CD45-CD235A-CD71-CD31-CD274+ BMSCs were sorted in TRIzol (Life Technologies) for bulk RNA sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; PAN-Biotech, Aidenbach, Germany, #P30-3306) and penicillin–streptomycin (100 U/ml; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, PAA Laboratories GmbH, Colbe, Germany) containing 10% fetal calf serum (FCS, Invitrogen, Carlsbad, California, USA), and 2 mM L-glutamine (PAA laboratories GmbH ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped by adding 100 µL H2SO4 (0.5 M) to each well and absorbances were measured in a microplate reader (Multiskan FC; Thermo Fisher Scientific) at 450 nm and 620 nm.
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (4 x 300 µL PBST) and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat ...
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bioRxiv - Immunology 2024Quote: ... we cultivated the corresponding rat hybridomas in IMDM (Gibco, 12440-053; supplemented with 10 % FCS, 1X MEM Non-essential amino acid solution (ThermoFisher, 11140050), 2 mM Glutamax (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for one hour in primary antibody NPM1 (FC-61991, ThermoFisher, 1 : 500 dilution in PBST with 1 %BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... and Rspo3-Fc Fusion Protein Conditioned Medium (Immunoprecise, #R001-100ml, 2%)] and mixed with 50 µl Matrigel (Fisher Scientific, #CB-40230C). Human alveolar organoid media was refreshed every 3 days until the end of the experiment ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). MA/cytBirA (24 ...
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bioRxiv - Immunology 2024Quote: ... placed static for 1 min at RT after which the absorbance at 450 nm was measured using the Multiskan FC Microplate Photometer (Thermo Scientific). A measurement was taken every 2 minutes for 50 minutes ...
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bioRxiv - Microbiology 2020Quote: Recombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...