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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Monocytes were cultured in 12-well plates at a density of 1 × 106/mL in Roswell Park Memorial Institute media 1640 (Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated TACC3 Δ699-765 (50 µL of 10 µg mL−1, diluted in PBS) was immobilized on preblocked HBC Streptavidin plates (ThermoFisher) for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... into a single-cell suspension and seeded on Matrigel-coated plates with ROCK inhibitor and knockout serum replacement (1%, Gibco).
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bioRxiv - Microbiology 2024Quote: ... cells were seeded in a 24-well plate at a density of 105 cells per well in 1 ml MEM (Gibco) and grown for 5-6 days ...
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bioRxiv - Microbiology 2024Quote: ... Vero cells (1 × 105 cells per well) in a 24-well cluster plate were transfected with 1 μg plasmid DNA using the Lipofectamine 3000 system (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The amplicons were checked by electrophoresis and 25 µl of each pooled PCR product were purified and normalized using SequalPrep Normalization Plate Kit (Invitrogen GmbH, Karlsruhe, Germany). We then pooled the samples and the mock community and proceeded for a single library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing reactions were carried out on 96-well plates using BigDye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems, foster City, CA) for 30 cycles ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from 10-day old seedlings grown on ½ MS plates under long-day conditions using PureLink RNA Mini Kit (Thermo Fisher Scientific, 12183025). One microgram of total RNA was reverse transcribed into cDNA with Protoscript II (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat. No. A1051001, ThermoFisher, Waltham, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was produced from mRNA from cells lysed directly in the culture plate using the TaqMan® Gene Expression Cells-to-CT™ Kit (ThermoFisher Scientific). qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 37°C for 24 hr and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: 10 μL of PCR product for each sample was normalized using a SequalPrep 96-well Normalization Plate Kit (Thermo Fisher Scientific, Waltham, MA). Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: DNA in 100 µl of undiluted cell-free BALF (first wash) was quantified in DNase-free 96-well plates (TPP®) using Quant-iTTM Pico GreenTM dsDNA Assay Kit (Thermo Fisher Scientific) following manufacturers’ instructions.
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bioRxiv - Zoology 2019Quote: Ten microliters of PCR amplicon was purified and normalized for concentration prior to library preparation using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, USA). DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Genomics 2022Quote: ... and split half for maintenance in a well of a 96-well plate and half used for genotyping using the Phire Animal Tissue Direct PCR Kit (Thermo Fisher Scientific, F140WH) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µL of each concentration was transferred into a U-shaped 96-well plate and incubated with 100 µL QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™) for 40 minutes along with the sEV samples to be detected on O-NEXOS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9 µL of the dPCR reaction mix was added to each well of a QuantStudio Absolute Q MAP16 Plate Kit (PN A52865, ThermoFisher Scientific, Waltham, MA). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for final quality assurance the CFFT Lab subjected all its plasmids from the daughter plate to next generation sequencing (NGS): DNA from plasmid constructs was quantitated using Qubit dsDNA HS Assay Kit (Q33230, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
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bioRxiv - Cell Biology 2024Quote: ... was used to measure collagen in lung lobes following kit instructions and optical density was measured using a Varioskan LUX plate reader (Thermo Fisher Scientific, VL0000D0).
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: 1 μL of RNA was assayed using the TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher, #4392938). Specific transcripts were detected using Taqman assays ...
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bioRxiv - Biophysics 2020Quote: ... 30,000 cells were seeded into a 24-well plate (NuncMicroWell Plates with Nunclon; Thermo Fisher Scientific, Waltham, MA). Transfection was carried out at day 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ninety-six-deep well plates and 500 cm2 cell culture plates were purchased from Fisher Scientific (Loughborough, UK). Millipore 96-well GF/B filter plates were purchased from Receptor Technologies (Warwick ...
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bioRxiv - Microbiology 2020Quote: ... and vancomycin in Mueller-Hinton broth using sensititre plates EUVENC Sensititre® plates (Thermo Fisher Scientific, Waltham, USA) and home-made 96-well microtiter plates for levofloxacin following the CLSI guidelines (19).
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bioRxiv - Immunology 2021Quote: ... to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
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bioRxiv - Cell Biology 2023Quote: ... 800,000 cells were seeded into a 6-well plate (NuncMicroWell Plates with Nunclon; ThermoFisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Microbiology 2023Quote: ... and 20 µl of the samples were distributed into 96-well plates (V-bottom storage plates, Thermo Scientific). Additionally ...
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bioRxiv - Microbiology 2023Quote: ... cells and supernatants or of mGAM were distributed into 96-well plates (V-bottom storage plates, Thermo Scientific) according to drug-treatment concentration ...
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bioRxiv - Immunology 2024Quote: ... Ninety-six well polystyrene plates NUNC MaxiSorp™ or NUNC 384-Well Clear polystyrene plates (Thermo Fisher Scientific) were coated with 2,4-dinitrophenyl conjugated to BSA (DNP-BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µg of whole cell lysates or 2 µg of Nuclear Fractions were boiled in loading buffer (NuPAGE LDS Sample Buffer; Thermo Scientific) with reducing agents (NuPAGE Sample Reducing Agent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was isolated from 20 μL of peripheral whole blood using a modified Tri-Reagent (Ambion, Invitrogen, Carlsbad, CA, USA) and Direct-zol RNA Miniprep Plus Kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was isolated from 20 μL of peripheral whole blood using a modified Tri-Reagent (Ambion, Invitrogen, Carlsbad, CA, USA) and Direct-zol RNA Miniprep Plus Kit (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... We isolated total RNA from the whole brain and liver of each animal using Trizol methods (Gibco-BRL, Gaithersburg, MD, USA), as directed by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... Whole genome sequencing (WGS) was performed using an Ion Torrent™ Personal Genome Machine™ (PGM) sequencer (Life Technologies, Carlsbad, CA) with 400-bp read chemistry according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA extraction was performed using whole hippocampi.34 RNA concentration was measured via a nanodrop Spectrophotometer (Thermo Scientific, Rockford, IL, U.S.A) and absorbance determined at a ratio of 260/280 ...
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Mis6/CENP-I maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosisbioRxiv - Cell Biology 2021Quote: ... The amounts of DNA derived from whole-cell extracts and ChIP samples were assessed via quantitative PCR on the StepOne Real-Time PCR system (Applied Biosystems) using SYBR Green (TOYOBO) ...
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bioRxiv - Bioengineering 2021Quote: ... Primary and highly cross-adsorbed whole goat IgG Alexa Fluor® (A-11029, A-21236, A-11034, A-21245; Invitrogen™) secondary antibodies were incubated ...
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bioRxiv - Cell Biology 2020Quote: For immunoprecipitation whole cell lysates or fractionated cytoplasmic lysates were incubated with indicated antibodies and Magnetic A/G beads (Thermo Scientific) or GFP-trap overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Whole cell lysates were separated by SDS-PAGE and blotted onto PVDF membranes using the NuPAGE system and reagents (Invitrogen™). Membranes were blocked in 5% [w/v] milk/TBST and then immunoblotted for ROCK2 (A300-046A ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×105 whole BM cells in 100µL PBS were spun onto charged microscope slides in a Cytospin™4 Cytocentrifuge (Thermo Fisher), air-dried and fixed in 100% methanol ...
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bioRxiv - Neuroscience 2022Quote: ... Optic nerves without retina were fixed with 4% PFA in PBS and whole-mounted on slides with Prolong™ Gold antifade (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: Endogenous tau was immunoprecipitated from whole lysates of differentiated SH-SY5Y cells (1mg) using 10μg of the HT7 tau antibody (ThermoFisher Scientific, #MN1000) and the crosslink magnetic immunoprecipitation kit (Thermo-Pierce ...
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bioRxiv - Neuroscience 2019Quote: Nearby PC pairs were whole-cell patched using an internal solution that included 50 μM of either Alexa-488 or Alexa-594 hydrazide (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell lysates and cytoplasmic fractions were quantified by measuring OD 280 nm in the Nanodrop 2000 Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Neuroscience 2021Quote: The whole extract and the cytosolic and synaptosome protein fractions were collected using the Syn-PER™ Synaptic Protein Extraction Reagent (ThermoFisher) following manufacture instructions ...
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bioRxiv - Plant Biology 2019Quote: ... and pigment absorption spectra of cell suspensions were measured in whole-cell cultures with a Genesys 10S UV-Vis Spectrophotometer (Thermo Scientific) and a Shimadzu UV-1800 UV spectrophotometer (Berner) ...
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bioRxiv - Cell Biology 2019Quote: Whole blood samples (300 uL) were diluted in 2.7 mL of PB-MAX™ Karyotyping media (Life Technologies, Grand Island, NY). Blood cultures were set up in a 48 well plate and cultured at 37°C in a humidified atmosphere with 5% CO2 for 44 h ...