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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Huh7 or Huh7-GCK+/HK2− were seeded at 1×105 cells per well in a 24-well plate in RPMI-1640 (Gibco) with 10% FCS and 40 μg/ml gentamycin ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS twice and incubated for at least 1 h with 20 µg/ml streptavidin (Thermo Fisher) in 1 % BSA/PBS at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS twice and incubated for at least 1 h with 20 μg/ml streptavidin (Thermo Fisher) in 1 % BSA/PBS at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were diluted 1:1000 and were added to the 2 mL liquid MSgg in 24 well plates (Thermo Scientific). The cultures were then grown at 30°C for 72 h in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... On day 7 EBs were transferred to ultra-low attachment 24 well plates and media was changed to neuroinduction media [1% N2 supplement (Gibco), 1% GlutaMAX (Life Technologies) ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Microbiology 2020Quote: ... or 1×104 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture-treated plates (Nunc) with a Multidrop Combi liquid handling instrument (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... T cells were seeded in 96 well plates at 1×106 cells/ml in the presence or absence of 2μg/ml of phytohaemagglutinin (PHA; Invitrogen, USA). Monocytes were seeded in 96 well plates at 1.5×106 cells/ml in the presence or absence of 10ng/ml of lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2021Quote: ... seeded in duplicate at 50 ng μL−1 (100 μL) in a Maxisorp plate (Nunc, Thermo Fisher Scientific, Waltham, USA) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 30,000 purified cells were stimulated in round-bottom 96-well plates with human anti-CD3/anti-CD28 T cell expander beads (1 bead/cell; ThermoFisher) in complete media containing 10 U/mL rhIL-2 with or without 10 or 20 uM 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO ...
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bioRxiv - Immunology 2020Quote: ... PBMC were stimulated by 0,1 μg/ml coated anti-CD3 (eBioscience) and incubated for four days in a 96 well round bottom plate (Nunc) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated at a density of 1×106 cells/well in a 24-well plate in 0.5 mL RPMI 1640 (Gibco, USA) culture medium alone ...
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bioRxiv - Cell Biology 2022Quote: ... and placed into the standard colony assay at a concentration of 1 cell/unit per well in a low-attachment Nunclon 96-well plate (ThermoFisher). Colonies were counted 2 weeks after plating ...
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bioRxiv - Developmental Biology 2022Quote: ... ESCs were grown for at least two weeks on fibronectin-coated plates (1/60 dilution in PBS) in 2i+VitC medium (N2B27 (50% Neurobasal medium and 50% DMEM/F12 (Gibco) complemented with 2mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... Cells in a 6-well plate were transfected with 2 μg of pTM1 P and 1 μg wt or mutant L using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were then stimulated with 50-100 ng/mL tumor necrosis factor (TNF; Miltenyi) or 100 μg/mL of the Dectin-1 ligand Curdlan (Wako biochemical) in flatbottom plates (Nunc) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The bacterial suspension was diluted 1:100 in triplicates into individual cell of a 24 well sterile plate (Nunc, Denmark) containing 2 mL of biofilm medium ...
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bioRxiv - Neuroscience 2024Quote: Neuro2A-cells (ATCC #CCL-131) were cultured in six-well plates (106 per well) in DMEM + GlutaMAX-1 (25300-054/096, GIBCO by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Immunology 2024Quote: ... IgGs from individual serum or pooled sera bound to the plates were detected after a 2-h incubation with goat anti– mouse-IgG–linked horseradish peroxidase (1:8,000 Invitrogen, USA). For measuring IgG isotypes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were grown in µ-Slide 8 well high ibiTreat (80806 Thistle Scientific) or 96-well plates to approximately 60% confluency and preloaded with 1 µM Fluo4-AM (F14201, Invitrogen) /0.02% Pluronic® F127 in HBSS ...
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bioRxiv - Developmental Biology 2024Quote: ... Five droplets were added to a 12-well plate and covered with 1 mL of TOM [Advanced DMEM/F12 (Gibco), 1x N2 supplement (Gicbo) ...
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bioRxiv - Immunology 2024Quote: ... plates were rewashed and incubated with HRP-conjugated goat anti-hamster IgG (H+L) secondary antibody (HA6007; Invitrogen; 1:1500) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Tissue was added to 96-well round bottom plates in 100μL of R10 (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, 1X Glutamax (Thermo Scientific), and 55μM b-mercaptoethanol) ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15 µL of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15ul of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Bioengineering 2024Quote: ... the plates were incubated with horseradish-peroxidase conjugated goat anti-mouse IgG (diluted 1/5,000) secondary antibodies (ThermoFisher 62-6520) for 1 h at room temperature ...
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bioRxiv - Biophysics 2024Quote: Fluorogenic POP substrate Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin (AMC) (HANGZHOU JHECHEM CO LTD) was diluted in PBS to 1-25 µM final concentrations in 96-well plates (ThermoFisher) to measure enzyme activity parameters in real time and as end-point reactions at 10 and 30 min ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Cell Biology 2023Quote: ... Both human and mouse hepatocytes were plated on collagen I coated tissue culture plate at a density of 1×105/cm2 in William’E medium (Thermo Fisher) and 10% FBS (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Genomics 2023Quote: ... EBs were plated on polyornithine/laminin-coated plates and cultured in N2/B-27 media containing 1 μg/mL laminin (Invitrogen). Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... stained bacteria were fed to ciliates using a 1:200 protist:bacteria ratio and transferred to a 96 well black plate (Thermo Scientific). DCF fluorescence was monitored automatically by measuring green fluorescence with 480 nm excitation and 512-552 nm emission wavelengths with a TECAN Infinite MPlex plate reader every 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Compound solutions were then dispensed at 10nl/well directly into columns 1–22 of 384-well assay plates (Nunc 242757), while columns 23 and 24 were reserved for controls (see below) ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Biochemistry 2022Quote: ... homogenized and counted to seed 7.5*105 cells in 1 mL of growth media per well in a 12-well plate (#55428, Nunc/DDBioLab) and incubated overnight at 23°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in double filtered sea water for 60 minutes on a shaking plate followed by three 1 minute 1X PBS Tween (1X Phosphate Buffered Saline: Gibco #70011-044 ...
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bioRxiv - Immunology 2023Quote: ... Between 1×106 and 1×107 splenocytes were transferred to 96-well plates for staining with freshly prepared LIVE/DEAD Aqua (Invitrogen) and TruStain FcX (anti-mouse ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: HepG2 cells and HepG2DNAJB1-PRKACA clones were transfected in 96-well plate with 0.25-100 nM Silencer small interfering RNA (siRNA) (Negative Control: #1, CDK7: s2830) (Life Technologies) using Lipofectamine RNAiMAX transfection reagent (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were thawed onto PLO and laminin-coated plates and cultured in astrocyte media supplemented with 1% knockout serum replacement (KSR)(Thermofisher). Astrocyte progenitor cells (APCs ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM EDTA pH 8.0) and purity was assessed using the Multiskan SkyHigh Microplate Spectrophotometer and µdrop plate (Thermo Scientific). Sample purity was assessed by measuring the 260/280 nm and 260/230 nm absorption and only samples achieving 1.7-2.0 and 2.0-2.2 respectively were further evaluated.
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: 293T IFNB12X-GLuc cells plated in 96-well plates were transfected with 1 pmol/well siRNA (Silencer Select, Thermo Fisher) using Lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was extracted from the femur and tibia of dissected mice and plated on non-tissue culture-treated 15-cm Petri dishes at a density of 1 x 107 cells per plate in macrophage differentiation medium (DMEM, Gibco) supplemented with 2mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
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bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
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bioRxiv - Immunology 2024Quote: Bd isolate JEL 197 was grown in 1% tryptone broth or on 1% tryptone agar plates (Difco Laboratories, Detroit, MI) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco) at 19°C.