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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from Caco-2 cells grown in 24 well plates for 21 days using the PureLink RNA Mini kit (Thermo Fisher, 12183025) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... aliquots of SAGs were transferred to replica plates for DNA yield quantification using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific). For sequencing analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed in 96 well plates and RNA was extracted using Applied Biosystems MagMax 96 total RNA isolation kit (Thermo Fisher, # AM1830) with on bead DNase digestion ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Immunology 2020Quote: ... Each plate was pooled and the plate libraries were quantified on a Qubit Fluorometer 3.0 using a dsDNA High-sensitivity Kit (Life Technologies, Carlsbad, CA). The plates were then pooled in equal volumes and the pooled library was then diluted to 6 μM and sequenced using a 500-cycle V3 reagent on an Illumina MiSeq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sizes were determined using an Agilent 2200 TapeStation system (∼550bp) and libraries normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific). Library concentration was calculated using a SYBR green quantitative PCR (qPCR ...
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bioRxiv - Physiology 2023Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of lactate in the reaction mix was measured with the Lactate Assay Kit (colorimetric assay) by reading the absorbance using Multiskan SkyHigh plate reader (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Pathology 2024Quote: The nephrotoxic effects of DMQ were investigated in the RPTEC cell line using a multi-parametric high content screening platform (Thermo Scientific Cell omics ArrayScan®) at eight-time points over nine days as described previously (Vormann ...
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bioRxiv - Biochemistry 2024Quote: ... Assay plates were generated by plating 2 µL of the deep well stock plates into 384-well assay plates (Thermo Scientific #142761 using the Agilent V11 Bravo). 88 µL of SrEpac cultured in high-nutrient media (5% Sea Water Complete ...
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bioRxiv - Microbiology 2020Quote: ... followed the whole protocol and the DNA concentration was measured before library preparation using a Qubit™ fluorometer (ThermoFisher Scientific). Negative controls did not produce measurable readings (estimated concentration <10 pg/µL).
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bioRxiv - Biophysics 2021Quote: Tilt series of whole or cryo-FIB milled mouse sperm flagella were acquired using a Titan Krios (Thermo Fisher Scientific) operated at 300 keV with post-column energy filter (Gatan ...
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bioRxiv - Cancer Biology 2021Quote: ... we assessed the baseline expression levels of TCF7L1 in 18 EwS cell lines for which whole-transcriptome data from Affymetrix Clariom D arrays was available (triplicates per cell line) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cochlear whole mounts and mid-modiolar sections were immunolabel for GFP-expressing macrophages (rabbit anti-GFP antibody, catalog #A11122, Invitrogen, 1:500 or chicken anti-GFP antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... The whole sample was then subject to cDNA synthesis using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific; Cat. No. 18080044), as directed by the manufacturer and employing oligo d(T)16 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... DNA extracted from whole blood and sperm was quality controlled using a Qubit 3.0 Fluorometer (Life Technologies, Cat No. Q33216). DNA was stored in −80°C prior to bisulphite conversion.
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bioRxiv - Immunology 2021Quote: Whole cell lysates (WCL) were prepared by resuspending cell pellets in RIPA lysis and extraction buffer (Thermo Fisher Scientific, #89901) containing a Protease/Phosphatase inhibitor cocktail (Cell Signaling Technology (CST) ...
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bioRxiv - Developmental Biology 2019Quote: ... equal amounts of otic vesicles or whole brain protein extract were resolved on NuPAGE 4-12% Bis-Tris Gels (Invitrogen) or NuPAGE 3-8% Tris-Acetate Gels (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: Whole blood samples from each dose point were cultured in PB-MAX™ Karyotyping media (Life Technologies, Grand Island, NY), and incubated at 37°C ...
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bioRxiv - Immunology 2019Quote: ... the blood system of the whole body was labelled by injecting 100 µl of WGA-Alexa Fluor 555 (5 mg/ml, Invitrogen) with 10 µl Heparin (100 units/ml ...
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bioRxiv - Genetics 2019Quote: ... and whole eyes that did not undergo FACS were used to generate cDNA with SuperScript III reverse transcriptase (Thermo Fisher). Each qPCR experiment included the following conditions at 150 pg cDNA per replicate ...
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bioRxiv - Genetics 2021Quote: ... whole brains were removed and incubated in a container of chilled RNALater buffer solution (Thermo Fisher Scientific, Waltham, MA, USA) for one minute before dissecting hippocampus and striatum ...
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bioRxiv - Bioengineering 2020Quote: 10 mL of whole blood from healthy donors was collected into BD SST serum tubes (Thermo Fisher Scientific, Waltham, MA). Tubes were rocked 10 times and then gently placed upright to coagulate for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... except for hyphopodia measurements where whole roots were gently removed and placed in nylon Biopsy Bags (Thermo Fisher Scientific, USA) inside tissue processing cassettes (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 μl of boiled supernatant of whole cell lysates was resolved on 4-12% Bis-Tris NuPAGE protein gel (Invitrogen) in 1×MES buffer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 100μg of whole cell lysate from each strain was run on NuPage 4-12% bis-tris protein gel (Thermo Scientific) at 120V for 3hours ...
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bioRxiv - Molecular Biology 2022Quote: The whole plasmid amplification strategy (WPA) reaction mixture contained 1x Phusion U Multiplex PCR Master Mix (Thermo Fisher Scientific, Lithuania), 0.06 μg of p426GPD-αfactor-αMSH plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... the image data from regions of interest spanning part or the whole moving dataset were warped into the target dataset’s space and rendered in a common viewport using Amira (Thermo Fisher) for illustrative purposes.
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bioRxiv - Cell Biology 2020Quote: ... HCT116 and SUM149PT whole cell lysates were isolated from spheroids grown for seven days using mammalian protein extraction reagent (M-PER; ThermoFisher) following manufacturer protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Fibrin formation was visualized by spiking whole blood with human fibrinogen conjugated to Alexa Fluor 647 (Cat. No. F35200, Invitrogen, used at final concentration of 7.5 μg/mL) ...
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bioRxiv - Immunology 2019Quote: Whole-cell extracts for immunoblotting were prepared by incubating cells for 10 min in RIPA lysis buffer (Thermo Fisher Scientific) with 50mM DTT and Protease/Phophatase inhibitor cocktail (Cell Signaling Technology) ...
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bioRxiv - Developmental Biology 2022Quote: ... 40 whole embryos at stage 3 (18 h AEL) were homogenized using disposable pellet pestles (12-141-368; Fisher Scientific) and 1.5 mL Eppendorf tubes (0030125150 ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted and purified from whole-animal homogenates using PureLink Genomic DNA Minikits (ThermoFisher Scientific Inc., Waltham, USA) and quantified using a Qubit dsDNA High-Range Assay Kit (ThermoFisher Scientific Inc. ...
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bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Immunology 2019Quote: ... Purified OTI CD8+cells or whole splenocytes from OT1 mice were labeled with Cell Proliferation Dye eFluor™ 450 (Invitrogen). After discarding the supernatant ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... Whole liver and hypothalamic mRNA were isolated from powered liver samples with TRI Reagent® (Life Technologies, Grand Island, NY) and phenol contamination was eliminated by using water-saturated butanol and ether as previously described 47 ...
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bioRxiv - Bioengineering 2019Quote: ... HCT116 and MDA-MB-231 whole cell lysates were isolated from flasks using mammalian protein extraction reagent (M-PER; ThermoFisher) following manufacturer protocols ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... One testis was randomly selected for whole tubule immunofluorescence and was immediately placed in cold PBS (Gibco, Cat# 10010-023) on ice ...
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bioRxiv - Genomics 2021Quote: Whole-cell lysates were prepared in RIPA buffer (Boston Bio-Products BP-115-500) with protease inhibitor cocktail (ThermoFisher 23225). Lysates were boiled in Laemmli buffer (BioRad 1610737) ...
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bioRxiv - Genomics 2021Quote: Whole-cell lysates were prepared in RIPA buffer (Boston Bio-Products BP-115-500) with protease inhibitor cocktail (ThermoFisher 23225). Lysates were boiled in Laemmli buffer (BioRad 1610737) ...
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bioRxiv - Physiology 2020Quote: ... The whole PAs determination was carried out by ultra-performance liquid chromatography (Ultimate 3000, Thermo Scientific, San Jose, CA, USA).
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bioRxiv - Immunology 2020Quote: Mononuclear cells were isolated by density centrifugation from whole blood and seeded at 106 cells/ml in Roswell Park Memorial Institute medium (RPMI (21875-034, Gibco)) ...
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections were prepared from whole ovaries at 6 µm thickness and placed on charged glass slides (Fisher Scientific). Ovary sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2021Quote: ... Samples with a minimum concentration of 33 ng/ul were selected for target amplification with the Whole Transcript (WT) Plus assay (Affymetrix). 100 ng of total RNA was used to process the samples for Whole Transcriptome Expression Analysis with the WT Plus assay.
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bioRxiv - Genomics 2020Quote: ... The extracted DNA was subjected to whole genome sequencing (WGS) using the Ion Torrent PGM sequencer (Life Technologies, Carlsbad, CA) with 400 bp read chemistry ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs from whole mouse blood were isolated with density gradient centrifugation and red blood cell (RBC) lysis (ACK lysing buffer, Gibco) and washed with cold 1X PBS ...