Labshake search
Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... from healthy donors were isolated from whole blood by density centrifugation on Lymphoprep (Axis-Shield PoC) and cryopreserved at −150 °C in FCS (Gibco) + 10% DMSO.
-
bioRxiv - Molecular Biology 2022Quote: Total protein was extracted from homogenized whole kidney lysates and RPTECs using mammalian protein extraction reagent (MPER, ThermoFisher Scientific, 78501) and Ambion PARIS kit ...
-
bioRxiv - Cell Biology 2022Quote: ... either from whole cells or from nuclear or cytoplasmic fractions was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treated with DNase I (Promega ...
-
bioRxiv - Cell Biology 2022Quote: ... either from whole cells or from nuclear or cytoplasmic fractions was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treated with DNase I (Promega ...
-
bioRxiv - Cell Biology 2022Quote: Western blots were carried out by running whole cell lysate or co-IP samples on a NuPAGE 4-12% BisTris gel (ThermoFisher) with MOPS running buffer ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA of whole bone with bone marrow flushed out from 4 weeks WT and DTAhet mice was extracted using Trizol reagent (Thermofisher), quantified and purified using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent) ...
-
bioRxiv - Immunology 2022Quote: Groups of 10 whole mosquitoes and 50 MT were dissected in PBS 10 at a time and were then transferred in Trizol (Invitrogen) in a 1.5 mL rubber gasketed screw-cap tube and frozen −80 °C ...
-
bioRxiv - Microbiology 2022Quote: We isolated RNA from one male and three female ticks using the same sterilizing and pulverizing steps as the whole-tick DNA protocol above but with 500 µL TRIzol Reagent (Invitrogen) following the manufacturer’s protocol and resuspending purified RNA in 15 μL lab-grade water.
-
bioRxiv - Cancer Biology 2024Quote: ... 50 µg of whole cell lysate was used for SDS-PAGE and then transferred to PVDF membranes (Thermo Scientific, PI88520). The membranes were blocked with 5% non-fat milk (Bio-Rad,1706404 ...
-
bioRxiv - Developmental Biology 2022Quote: ... crypt-enriched and dissociated to a single cell suspension as described previously.23 Developmental samples were dissociated by mincing whole small intestinal tissues in 1x TrypLE-express digestion reagent (Gibco) supplemented with 50ug/mL DNAseI (Roche ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10-30ug of whole cell lysate was loaded into each well of 4-12% Bis-Tris NuPage polyacrylamide gels (Invitrogen). Proteins were transferred overnight onto PVDF membranes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
-
bioRxiv - Cell Biology 2024Quote: ... Bound proteins and input samples (whole cell lysates) were loaded on Novex NuPAGE 4-12% gradient Bis-Tris gel (Invitrogen) and transferred to Immobilon-P transfer membranes ...
-
bioRxiv - Immunology 2024Quote: ... whole blood sample was diluted to three times its volume with a solution of PBS 1X (Gibco, cat #10010-015) supplemented with EDTA at 0,2 mM (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... Whole cell lysates were made by lysing cells with RIPA buffer containing Halt protease and phosphatase inhibitor cocktail (ThermoFisher, 78440) and Dnase I (ThermoFisher ...
-
bioRxiv - Cell Biology 2023Quote: ... 500 µg of whole protein was incubated with 100 µL superparamagnetic beads covalently coupled with streptavidin (MyOne Streptavidin C1; Invitrogen) overnight at 4°C.
-
bioRxiv - Microbiology 2023Quote: ... For STEVOR assay, eluates containing DNA (from 25 μL whole blood, or 2 μg DNA from liver or spleen (Nanodrop 2000, ThermoFisher)) were analyzed as described previously (48 ...
-
bioRxiv - Immunology 2022Quote: ... 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher) as described (Wu et al. ...
-
bioRxiv - Molecular Biology 2023Quote: Whole cell extracts were resolved using a Novex™ WedgeWell™ 6% Tris-Glycine Mini Protein Gel (Thermo Fisher XP00065BOX). Samples were transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Systems Biology 2023Quote: Whole-cell lysates were prepared in RIPA buffer (Boston Bio-Products BP-115-500) with protease inhibitor cocktail (ThermoFisher 23225). Lysates were boiled in Laemmli buffer (BioRad 1610737) ...
-
bioRxiv - Molecular Biology 2023Quote: Whole-cell lysates were prepared in RIPA buffer (Boston Bio-Products BP-115-500) with protease inhibitor cocktail (ThermoFisher 23225). Lysates were boiled in Laemmli buffer (BioRad 1610737) ...
-
bioRxiv - Developmental Biology 2023Quote: ... R 5’-atggatccTCATAGAGCTGAAGCCACCAG-3’) were generated by PCR amplification from 24 hpf whole embryo cDNA and cloned to pCRII-TOPO (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Whole organoids were plated in 10% Matrigel and HISC media on chamber slides with removable wells (Thermo Fisher Scientific, 177380), and incubated at 37°C for 1 hour ...
-
bioRxiv - Genomics 2023Quote: ... Embryos were harvested at E18.5 and whole liver protein extracts were prepared in Cell Lysis Buffer (CLB, Thermo Fisher Scientific). Protein extraction was further facilitated by dounce homogenization ...
-
bioRxiv - Developmental Biology 2023Quote: ... The stained tissue was either mounted as whole mounts or cryo-sectioned immediately and mounted with ProLong Gold Antifade (ThermoFisher).
-
bioRxiv - Physiology 2023Quote: Gene expression profiling of RNA isolated from whole lung tissue was performed using the GeneChip® Mouse Gene 2.0 ST Array (902118, Affymetrix). A custom CDF version 22 with ENTREZ-based gene definitions was used to annotate the arrays ...
-
bioRxiv - Neuroscience 2024Quote: ... Euthanasia was followed by a quick decapitation of the animals and the whole hippocampi was harvested in Trizol reagent (Invitrogen) for total RNA isolation ...
-
bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
-
bioRxiv - Immunology 2024Quote: For Western blotting whole cell lysates were fractionated by 10% Bis-Tris polyacrylamide NuPAGE gels (Thermo Fisher Scientific, Waltham, MA) and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer’s protocols (Bio- Rad ...
-
bioRxiv - Microbiology 2024Quote: ... Segment-specific RT-PCR was performed to amplify whole gene segments for sequencing by first synthesizing cDNA using SuperScript III (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... the plate was then read at 450 nm using a Multiskan® EX plate reader (Thermo Scientific), and the data analysed in GraphPad Prism version 7.0c ...
-
bioRxiv - Bioengineering 2021Quote: ... Fluorescence readings were conducted using a Spectramax plate reader in a black-walled clear bottom plate (Nunc), with the excitation/emission for TO1 and TO3 being 510/535 nm and 637/658 nm ...
-
bioRxiv - Microbiology 2021Quote: ... pneumoniae (one colony per species per plate) were further sub-cultured on Sheep Blood Agar plates (ThermoFisher) and species identity confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ...
-
bioRxiv - Neuroscience 2021Quote: ... the plate was washed 4 times with PBST using a plate washer (Thermo Fisher Scientific Wellwash Versa), and anti-pTRKB antibody (1:1000 in the blocking buffer ...
-
bioRxiv - Plant Biology 2023Quote: Stratified seeds were germinated on square plates (100 mm × 100 mm square plates; Fisher Scientific; Cat#FB0875711A) containing sterile full-strength (1x ...
-
bioRxiv - Biochemistry 2024Quote: ... if a 96-well plate (SureSTARTTM WebSealTM 96-Well Microtiter Plate, SN: 60180-P210B, Thermo Fisher Scientific) was used for injection ...
-
bioRxiv - Microbiology 2023Quote: ... the plate was transferred to 20° C pre-cooled plate reader (Multiskan GO, Thermo scientific, Waltham, MA) and shaken slowly for 10 seconds ...
-
bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
-
bioRxiv - Biochemistry 2023Quote: ... using the protocol according to the silver staining kit (SilverQuest Silver Staining Kit-1, LC6070, Life Technologies Ltd) and the purified protein was aliquoted and stored at −80°C until use.
-
bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
-
bioRxiv - Neuroscience 2020Quote: The level of Aβ in the tissue was assessed by ELISA as per the manufacturer’s instructions (KHB3442, Invitrogen). The resulting measurements were normalized for tissue weight.
-
bioRxiv - Neuroscience 2021Quote: ... were prepared for ELISAs by coating each well with 50ul of 1.5ug/ml HT7 monoclonal antibody (Thermo Fisher) the night before use ...
-
bioRxiv - Neuroscience 2019Quote: ... and results of the ELISA were measured by a colorimetric microplate reader (VarioSkan 2.2; Thermo Scientific Inc., USA) at 450nm ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were pelleted (400xg, 10 min) and supernatants subjected to ELISA analysis for IL-17a (Thermo Fisher, UK).
-
bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... 200 ng/ml) and medium was collected for analysis of the amount of secreted TNFα by ELISA (Invitrogen).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The concentration of Aß40 and Aß42 in the culture medium was quantified by ELISA (Thermo Scientific, Waltham, MA). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... supernatants were collected for cytokine analysis by ELISA (IL-2 BD-OptEIA™; IL-17A, IFN-γ ThermoFisher) following manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.