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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Molecular Biology 2019Quote: Whole cell protein extraction for western blot was done using Radioimmunoprecipitation assay (RIPA) buffer (cat#89900, Thermo Fisher Scientific, MA, US) using the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of whole cell lysates (20 μg protein per sample) were denatured in Tris-Glycine SDS Sample buffer (ThermoFisher LC2676), and loaded on a Novex 4-20% Tris-Glycine gel (ThermoFisher XP04205BOX) ...
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bioRxiv - Genomics 2021Quote: Imaginal discs and brains were dissected from at least six larvae of each genotype and immunostained with rabbit serum against CP190 following whole-mount staining methods as previously described28 mounted with ProLong Diamond mounting media (Life Technologies). Number of insulator foci per nucleus were counted manually.
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bioRxiv - Developmental Biology 2021Quote: ... Whole testes were mounted on polylysine coated slides in TIB and squashed with a coverslip (Polysine, P4681-001, Thermo Fisher Scientific). Live images for Fig ...
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bioRxiv - Developmental Biology 2021Quote: ... whole mount duck and quail embryos were stained with Hoechst 33342 Dye (62249, Lot# RF22228410, ThermoFisher Scientific, Hanover Park, IL, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... we analyzed DNA derived from peripheral blood and a skin biopsy collected on the same day using Whole Genome Sequencing (WGS) using SOLiD sequencing technology (Life Technologies) at 80x mean depth (Supplementary Methods 2) ...
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bioRxiv - Immunology 2021Quote: ... and 10-30 μg total protein from whole-cell lysates was run on SDS–PAGE and transferred to polyvinylidene difluoride membranes (Thermo Scientific). The membranes were blocked in 5% milk in PBS-T (pPBS/Tween 20) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates from induced (day 2) and non induced TAP110 overexpressing whole cells were separated on 10% NOVEX gradient SDS gel (Thermo Scientific) for 8 min at 180V in 1x MES buffer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: Whole-cell lysates were obtained incubating cell pellets with a Lysis Buffer (2% n-dodecyl β-dmaltoside (DDM) (Thermo Scientific, #89903) plus 1X protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was extracted and either embedded in 4 % agar for automated whole-brain imaging or cut coronally into 100 µm sections using a vibratome (Microm HM 650V, Thermo Scientific). Coronal sections were mounted in a medium containing DAPI (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and the whole transcriptome of ALDH+ and ALDH-populations was assessed using Affymetrix Whole-Transcript Human Gene 1.0 ST Array (Affymetrix, Thermo Fisher Scientific) (30) ...
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bioRxiv - Cell Biology 2022Quote: Samples for western blot analysis taken during co-immunoprecipitation (“whole cell lysate”, “flow-through”, “input” and “eluate”) were each supplemented with NuPAGE™ LDS Sample Buffer (4X) (NP0007, Invitrogen), 50 mM DTT and 5 % beta-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... Whole blood was diluted 2-fold in calcium- and magnesium-free phosphate-buffered saline (PBS pH: 7.2; Fisher Scientific, Waltham, MA) within 30 min of collection ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Whole cell lysate was extracted on ice by first resuspending the cell pellet with Pierce IP Lysis Buffer (ThermoFisher Scientific; 87787) supplemented with cOmplete protease inhibitor cocktail (Roche ...
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bioRxiv - Systems Biology 2022Quote: The whole kidney was homogenized and nuclear fraction was extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific, # 78833), following the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: 10 mL of whole blood from healthy donors was collected into BD Serum Separation Tubes (SST) (Thermo Fisher Scientific, Waltham, MA). SSTs were gently placed upright to coagulate for 60 min after being rocked 10 times ...
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bioRxiv - Immunology 2022Quote: BMDMs were lysed with RIPA buffer and the whole-cell protein lysates were separated on pre-cast Bolt 12% Bis-Tris Plus gels (Invitrogen, NW00127BOX) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: Microdissected cochlear whole mounts or frozen mid-modiolar cross sections (20 μm) were rinsed with PBS (Fisher scientific, cat. # BP661-10) at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Whole-cell extracts (1e6 cells) were solubilized with 2 mg of digitonin in native PAGE sample buffer (Thermo Fisher Scientific, BN2008) for 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of whole protein extracts were loaded and separated by electrophoresis on a 4–12% Bis-Tris gel (Life Technologies) and transferred to a PVDF membrane (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Endogenously TAP-tagged versions of the proteins of interest were immunoprecipitated by incubation of whole cell lysate with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific) for 2.5 h at RT ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Whole cell and nuclear compartment were lysed with 8 M Urea buffer containing a protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) and were sonicated for 15 seconds at 60% amplitude ...
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bioRxiv - Neuroscience 2023Quote: ... and whole media fractions were mixed with the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (0.33 ng/ul, Life Technologies, O246) and incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of the whole cell lysate was loaded on 15% resolving gel and subjected to mouse primary anti-FLAG antibody (Thermo Scientific) and secondary goat anti-mouse HRP-conjugated antibody (Cell Signalling Technology).
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of total protein (from whole cell extracts or isolated lysosomes) was separated with a 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and then transferred onto PVDF (polyvinylidene difluoride ...
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bioRxiv - Molecular Biology 2024Quote: ... and whole tissue lysates from the other studies were quantified using bicinchoninic acid (BCA) colorimetric assays (Thermo Scientific, Waltham, MA, USA). Isolates and lysates from all studies were then prepared for Western blot analysis with 4× Laemmli buffer for final concentration preparations at 1 μg/μL ...
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bioRxiv - Immunology 2024Quote: ... Biotin-coupled DNA-protein complex was pulled down by incubating whole cell lysates with high-capacity streptavidin agarose beads (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 96-well glass-bottom plates (Thermo Scientific) 48 hours before imaging ...
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bioRxiv - Immunology 2021Quote: Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well ...
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bioRxiv - Immunology 2020Quote: ... 96-well plates (Thermo Fisher Scientific, USA) were coated per well with 250 ng of individual recombinant proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... protein adsorbing 384-well microtiter plates (Nunc® MaxisorpTM ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were sealed (Thermo Fisher Scientific #AB0626) immediately spun down and frozen at −80 °C until further processing.
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bioRxiv - Immunology 2022Quote: ... 96-well MaxiSorp plates (Thermo Scientific #442404) were coated with 50 μL/well of recombinant SARS-CoV-2 S1 protein (ACRO Biosystems S1N-C52H3 ...
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bioRxiv - Microbiology 2022Quote: ... ProcartaPlex magnetic bead cytokine/chemokine plates (Invitrogen) were prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... on six-well Nunclon surface plates (Nunc) coated with Geltrex (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in feeder-free conditions in Matrigel (Corning)-coated plates in StemFlex medium (Thermo Fisher). To produce organoids ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Black 96-well plates (Thermo Scientific, #7805) were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB ...
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bioRxiv - Immunology 2019Quote: ... 96-well Nunc MaxiSORP plates (Thermo Scientific) were pre-coated with methylated BSA (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... 96-well PCR plates (AB0600, Thermo Scientific) were prepared with 3.52 (4.4 ...
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bioRxiv - Neuroscience 2019Quote: ... in 6-well culture plates (ThermoFisher Scientific) with 800 µL of sterile medium added below the insert (Stoppini et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Medisorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 10 μg/mL protective antigen in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... in 96-well PCR plates (Applied Biosystems). FAM/MGB-NFQ fluorescence emissions were monitored after each cycle ...
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bioRxiv - Cell Biology 2020Quote: ... in 96-well PCR plates (Applied Biosystems). SYBR Green or FAM/BHQ-1 fluorescence emissions were monitored after each cycle ...
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bioRxiv - Immunology 2020Quote: ... 96 well flat-bottomed plates (Nunc, #439454) were coated with Sm/RNP (ImmunoVision ...
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bioRxiv - Immunology 2020Quote: ... 96 well flat-bottomed plates (Nunc, #439454) were coated with anti-IgG-FC overnight at room temperature ...
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bioRxiv - Genetics 2019Quote: ... low-binding culture plate (Thermo Fisher Scientific) for 2 days in GMEM supplemented with 15% Knockout serum replacement (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... 4X 14 cm plates (Nunc, Roskilde, Denmark) of HUVEC were required for each proteomics condition analysed ...
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bioRxiv - Biochemistry 2019Quote: Nunc MaxiSorp™ plates (Thermo Fisher Scientific) were coated overnight with 50 μl of substrates ...