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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Expression vector was transiently transfected into Human Embryonic Kidney (HEK) 293 GnTI-Freestyle cells suspension culture in serum-free media (Invitrogen) using polyethyleneimine (Polysciences) ...
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bioRxiv - Immunology 2021Quote: ... as a Cas9-RNP complex (1:1 Cas9:gRNA molar ratio) were delivered primary human CD3+ T cells after 2 days of Anti-CD3/-CD28 Dynabead (ThermoFisher) stimulation ...
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bioRxiv - Genomics 2021Quote: ... we stimulated these CD4+ T cells with CD3/CD28 crosslinking for 45 hours (Gibco, Dynabeads Human T-Activator CD3/CD28). We then performed ChIP-seq (2 million stimulated CD4+ T cells) ...
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bioRxiv - Genomics 2021Quote: The pulmonary HLA-DR and CD3-positive cells were isolated using superparamagnetic beads coupled with anti-human CD3 and Pan Mouse IgG antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Microbiology 2021Quote: Human endothelial kidney (HEK 293) and murine fibroblast (3T12) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Life Technologies) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... 60 mm dishes of BHK-21 cells were transiently transfected with 2.5 μg human ACE2 or no DNA for control using standard lipofectamine 2000 (Life Technologies) protocols ...
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bioRxiv - Cell Biology 2022Quote: ... cells were then incubated with pre-warmed DMEM containing 20 µg/ml of TxR-Tfn (from human serum, Molecular Probes) and 0.1 % BSA for the specified times ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... the pFastBac plasmid containing full-length human dynein or tail-truncated dynein was transformed into DH10Bac competent cells (Gibco, #10361012) with heat shock at 42 °C for 45 seconds followed by incubation at 37 °C for 4 hours in S.O.C ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... then co-cultured at the indicated ratios and activated with Dynabeads Human T-Expander (1 bead:16 CD3+ T cells; Gibco) for 4 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... cryopreserved PBMCs or T cells were thawed on day 0 and cultured with Human T-Activator αCD3/CD28 Dynabeads (Gibco) at 3:1 bead:cell ratio in AIM-V media (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... CVCL_1926) and HEK293T cells stably expressing human ACE2 (BEI NR-52511, RRID: CVCL_A7UK) were maintained in DMEM (Gibco, 11965-092) with 10% FBS (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Huh7 human hepatoma and 293T cells (Riken, Japan) were grown and maintained in Dulbecco’s modified Eagle medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: African green monkey kidney cells (Vero) and Human Embryonic Kidney 293T (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Genomics 2024Quote: ... human chronic myelogenous leukemia cancer cell line K562 and human embryonic epithelial kidney cell line HEK293T were cultivated in Dulbecco’s Modified Eagle Medium with high glucose (#11965084, Life Technologies) supplemented with 10% fetal bovine serum Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Human intestinal epithelial cells (HIEC-6) were cultured in Opti-MEM™ I Reduced Serum Medium (Thermo Scientific, MA, USA). Cell viability was measured using MTT assays (ref) ...
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bioRxiv - Cell Biology 2023Quote: ... human pluripotent stem cells were dissociated using Accutase and plated at high density on Geltrex (#A14133-02, Thermo Fisher Scientific) coated wells ...
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bioRxiv - Cancer Biology 2023Quote: ... Viably frozen primary AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24h before transplant ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labelled with phycoerythrin (PE)-conjugated anti-human-HLA-ABC (clone W6/32, Invitrogen 12-9983-42; 1:20), biotin-conjugated anti-mouse-H-2Kb (clone AF6-88.5 ...
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Immunology 2023Quote: ... purified Treg cells (5 × 104) were cultured with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Genomics 2023Quote: The human lung fibroblast cell line IMR-90 was purchased from ATCC and cultured in EMEM (ATCC) with 10% FBS (Gibco) and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Unbound ACE2-Fc was removed by washing the cells with ice cold media and the bound ACE2 detected using goat anti-human secondary antibody conjugated to Alexa647 (ThermoFisher Scientific Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Genomics 2023Quote: Pulmonary arterial adventitial cells isolated from idiopathic PAH and control lungs were obtained from PHBI and grown in Human Vascular Smooth Muscle Cell Basal Medium (M231500, ThermoFisher) supplemented with Smooth Muscle Growth Supplement (S00725 ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... in 6-well tissue culture plates at 37°C with 5% CO2 and 5% O2 They were grown in human embryonic stem cell (hESC-10) medium made up of a base of DMEM/F12 (50:50 Gibco) with 20% knock-out serum replacement (KOSR ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: NIH 3T3 immortalised mouse fibroblast and U2OS human osteosarcoma cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Catalog# CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Microbiology 2023Quote: ... Detroit 562 human nasopharyngeal epithelial cells (ATCC ref# CCL-138) were maintained in DMEM supplemented with 1x sodium pyruvate (Gibco) and 1x GlutaMax (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ cells were combined at a 1:1 ratio and activated using anti-human CD3/CD28 Dynabeads (Invitrogen, #40203D) at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: Human A549 or A549ACE2 cells were cultured in a Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco catalog no. 11875) supplemented with 10% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: DLD 1 human colorectal cancer and Colon26 mouse colon cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)(Gibco, USA) containing 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 (anti-human CD3 for NK cell experiments) according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Immunology 2023Quote: ... 293T cells and human lung epithelial A549 cells (both originally from ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific), supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... human ileocecal cell line from 25 to 35 passages were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 4mM L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Monkey kidney epithelial cells Vero E6 (clone CCL81) and human lung epithelial cells Calu-3 were respectively cultured in DMEM medium and EMEM medium (ThermoFisher) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...