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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... NOK1 cells were grown in keratinocyte-SFM medium supplemented with human EGF and bovine pituitary extract (BPE) (Thermo Fisher Scientific) and induced to differentiate by transfer to Dulbcecco modified Eagle medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: Human non-transformed retinal pigment epithelial cells transduced with the human telomerase gene (hTERT-RPE1; ATCC CRL-4000) were grown in DMEM/F12 (Gibco) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2020Quote: ... 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen), FITC-conjugated anti-human CD4 (BD Bioscience) ...
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bioRxiv - Biophysics 2021Quote: ... ATCC) and HeLa (a human cervical cancer cell line, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Microbiology 2021Quote: ... Caco-2 (human epithelial colorectal adenocarcinoma, kindly provided by Prof. Holger Barth, Ulm University) cells were cultivated in DMEM (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were from A Human Glioblastoma Cell Culture Resource (HGCC) and were cultured as described (Xie et al., 2015) in Neurobasal medium (GIBCO)/DMEM/F12 with Glutamax (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
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bioRxiv - Bioengineering 2022Quote: ... immortalized human dermal fibroblast cell line (Stephens et al., 2004) were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco™) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... zsGreen/Oatp1b3) into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s lentiviral production protocol (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2022Quote: Cardiac differentiation of human iPS cells was performed using a stage-based protocol in RPMI-1640 (ThermoFisher Scientific, Waltham, MA) supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... mature human small intestinal colonic organoids grown with the expansion media were digested into single cells using TrypLE Express (ThermoFisher) and seeded into Matrigel-coated 96-well culture plate (Corning ...
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bioRxiv - Molecular Biology 2022Quote: Wild type and MED14-dTAG human colorectal carcinoma HCT-116 cells [25] were grown in RPMI 1640 medium (Gibco, 21875034) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco™, Cat no. #12800017) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
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bioRxiv - Cancer Biology 2022Quote: ... as well as NCM-356 (ATCC CVCL-D875) non-transformed human colonic mucosal cells were maintained in McCoy’s media (Thermo Scientific) supplemented with 10% FBS (Gemini Bio-Products) ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Neuroscience 2020Quote: ... and the SH-SY5Y human neuroblastoma cell line was maintained in 10% FBS Minimum Essential/F12 medium (MEM/F12, Gibco). Both cells’ media included 1% antibiotic/antimycotic mix (Gibco) ...
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bioRxiv - Microbiology 2021Quote: Individual plasmids encoding 11 human genes or empty control vector were transfected individually into U-87 MG cells with Lipofectamine 3000 (Invitrogen) 48 hours prior to infection with VEEV-TC-83-nLuc.
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bioRxiv - Immunology 2019Quote: ... T cells were stimulated for 2 days at a starting density of approximately 1 million cells per mL of media with anti-human CD3/CD28 Dynabeads (ThermoFisher), at a bead to cell ratio of 1:1 ...
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bioRxiv - Microbiology 2019Quote: ... and HEK293T (embryonic human kidney epithelial, ATCC®CRL-3216) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... The immortalized human upper airway epithelial cell line A549 (ATCC# CCL-185) was maintained in minimal essential medium (MEM; Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Genomics 2020Quote: Female human bone osteosarcoma U-2 OS cells (ATCC HTB-96) were routinely cultured in McCoy’s 5A (Modified) medium (ThermoFisher 16600) with 10% FBS (Gemini Bio ...
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bioRxiv - Molecular Biology 2021Quote: Human proximal renal tubular epithelial cells (HK2) were purchased from ATCC (ATCC®CRL-2190) and cultured in DMEM (Gibco) supplemented with 10% FBS (Moregate) ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 (Apath) cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, 10-013-CV); African green monkey kidney Vero (ATCC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then washed and incubated with PE-conjugated goat anti-human Fc antibodies (Thermo Fisher 12-4998-82) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... mosquitoes were offered a sterile bloodmeal consisting of 50 % human red blood cells (Etablissement Français du Sang de Guadeloupe-Guyane) and 50 % foetal bovine serum (Thermo Fisher). The bloodmeal was provided to mosquitoes in an autoclaved Hemotek reservoir (Hemotek ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Genomics 2021Quote: A total of 10 technical replicates of human neuroblastoma SH-SY5Y cells were cultured in neurobasal media (Gibco 21103-049) supplemented with B-27 Plus (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... CPA and CPD cells were cultured in Keratinocyte-SFM supplemented with 2.5 μg prequalified human Epidermal Growth Factor 1-53 (EGF) (Life technologies), 25 mg Bovine Pituitary Extract (BPE ...
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bioRxiv - Microbiology 2020Quote: ... kind gift from Peter Bredenbeek, LUMC, NL) and HuH7 (human hepatoma, JCRB0403) cells were maintained in minimal essential medium (Gibco) supplemented with 10% fetal bovine serum (Integro) ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells adapted to suspension culture (HEK293F) were grown in Free Style 293 Expression Medium (Thermo Fisher Scientific) and harvested at a density of 3 million cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... falciparum cell lines were cultured in human O+ RBCs (Australian Red Cross) with RPMI 1640 medium with GlutaMAX and HEPES (ThermoFisher), supplemented with 5% v/v human serum (Australian Red Cross) ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-N-FI) and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... of SARS-Cov-2 (Genbank accession number YP_009724390.1) was codon optimised for expression in human cell lines and synthesised by GeneArt Gene Synthesis (Thermo Fisher Scientific). The sequence encoding amino acids 2-1273 were cloned into a shuttle plasmid following InFusion cloning (Clontech) ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Microbiology 2019Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney HEK293T (293T) cells were maintained in Gibco’s minimal essential medium with GlutaMax (Life Technologies). Human lung epithelial A549 cells were maintained in Gibco’s F-12 medium (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The human hepatocellular carcinoma HepG2 cell line (ATCC HB-8065) and the human kidney adenocarcinoma A-704 cell line (ECACC 93020513) were grown in minimum essential medium (MEM, Gibco) with glutamax supplemented with 10% FBS ...