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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
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bioRxiv - Molecular Biology 2020Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, Waltham, MA, USA; Cat#10566016) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... of human CD58 (UniProt residues 29-–204 + AviTAG + 6xHis) and human CD86 (UniProt residues 24–238 + AviTAG + 6xHis) were produced in Freestyle 293F suspension cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney HEK293T (293T) cells were passaged and maintained in Minimum Essential Medium (MEM + GlutaMAX, ThermoFisher Scientific) with 8.3% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2019Quote: ... The poly(A)-tailed RNA in human CCRF-SB cells was isolated from the total RNA using a Fasttrack MAG Maxi mRNA isolation kit (Life Technologies), whereas the poly(A)-tailed RNA in yeast cells and mouse tissues was isolated from the total RNA using a Dynabeads mRNA Purification kit (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... or 1×105 (96-well plate) naïve CD8+ T-cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 beat to T-cell ratio and cultured in 1 ml (48WP ...
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bioRxiv - Genetics 2021Quote: ... into the melanoma cell line UACC 903 and multiple human melanocyte cultures (c23, c197 and c87) using Lipofectamine 2000 reagent (Thermo Fisher) for UACC903 or electroporation with the Lonza Amaxa P2 kit and the CA-137 protocol on the 4D-Nucleofector system for primary melanocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Immunology 2020Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Immunology 2021Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Molecular Biology 2021Quote: ... The human laryngeal cancer cell line (HEp2) was purchased from ATCC (CCL-23) and cultured in DMEM/F12 medium (Gibco, 11320033) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 2×106 cells were cultivated for 24 h in presence of either Human T-Activator CD3/CD28 dynabeads (Thermo Fisher Scientific), according to manufacture’s instruction ...
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bioRxiv - Physiology 2021Quote: Human embryonic kidney 293T (HEK293T; ATCC, Manassas, VA, USA) cells were cultured in Opti-MEM I media (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue culture flasks until they reached 70-80% confluence in human ectocervical primary cell medium (Advanced DMEM/F-12 (Invitrogen, 12634) supplemented with 12 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant poly-histidine tagged ectodomains of the SARS-CoV-2 S proteins (614D and 614G) were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using HisTrap FF column (GE Life Sciences)18 ...
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bioRxiv - Cell Biology 2020Quote: Human pluripotent stem cells were cultured and maintained on feeder-free plates with StemFlex Medium (Cat. No. A3349401, Thermo Fisher Scientific) as described 58 ...
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bioRxiv - Genetics 2021Quote: Human-derived cell line THP1 (ATCC TIB-202) was cultured in RPMI medium with 10% fetal bovine serum (FBS, GIBCO, USA). THP1 cells were activated with 100 ng/ml phorbo-12-myristate-13-acetate (PMA ...
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bioRxiv - Cell Biology 2019Quote: MCF10A human mammary epithelial cells were a kind gift from Sabrina Spencer and were cultured in growth medium DMEM:F12 (Gibco, #11320-033) containing 5% horse serum (Gibco ...
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bioRxiv - Immunology 2021Quote: ... or 1×105 (96-well plate) naïve CD8+ T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 bead to T cell ratio and cultured in 1 ml (48-well plate ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2021Quote: ... The human leukemia monocytic cell line THP-1 16 was cultured in Milieu Roswell Park Memorial Institute (RPMI) medium (ThermoFisher Scientific) enriched with 10% fetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Secreted factors were measured in the T cell assay culture media using a Luminex kit (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific) per manufacturer’s recommendations with 4 replicates per sample ...
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and resuspended in phenol-free RPMI with 2% (v/v) human AB serum containing DAPI (ThermoFisher 62248). Single-color controls were performed with these same antibodies using BD CompBeads (BD Biosciences 552843) ...
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bioRxiv - Microbiology 2020Quote: ... human epithelial cell line were cultured at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, Waltham, MA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs were extracted from the human prostate tumors and the normal tissues and various cell lines using Trizol (Life Technologies). Reverse transcription was performed using the M-MLV reverse system (Takara ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2.5 µg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes [65] using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2/UT-NCGM02/Human/2020 (Wuhan strain) was propagated in VeroE6/TMPRSS2 cells in VP-SFM (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... Human peripheral blood mononuclear cells (ATCC #PCS-800-011) were thawed as recommended and resuspended in RPMI 1640 medium (Gibco #11875093) with 1X GlutaMAX (Gibco #35050061) ...
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bioRxiv - Cancer Biology 2024Quote: ... #D-001810-10-05) or human GPX3 siRNA (Dharmacon, #L-006485-00-0005) were delivered to the cells using Lipofectamine RNAiMAX (Invitrogen, 13778150). After 24 hours ...
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bioRxiv - Neuroscience 2023Quote: eGFP/Luc human iPSC-NSCs were seeded at a density of 1 x 104 cells per well in a Geltrex (Life Technologies) coated 96-well plate (flat bottom ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: Cytokine secretion of PMA treated THP-1 cells was analyzed using Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) and measured with xMAP LuminexTM system (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Human TS cells were cultured in six-well plates precoated with 5 μg/mL of CorningTM collagen IV (CB40233, Thermo Fisher) and Basal Human TS Cell Medium [Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12 ...
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bioRxiv - Genomics 2024Quote: ... and were activated for 72h using Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermofisher, 11161D) according to the manufacturer’s instructions at 37°C under 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... and were activated for 72h using Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermofisher, 11161D) according to the manufacturer’s instructions at 37°C under 5% CO2 ...
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bioRxiv - Immunology 2024Quote: Human monocytic cell line THP1 (TIB-202, ATCC, Manassas, VA, USA) were seeded 106/ml on glass chamber slides (Thermo Scientific) precoated with poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Bioengineering 2024Quote: Human fibroblast (ATCC) and SKOV-3 cells (graciously donated from the Dr. Hoshino lab) were cultured on T75 flasks (Fisher Scientific) at 5% CO2 at 37° C using McCoys 5A media (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and PANC-1 (CRL-1469, ATCC) and the human colorectal cancer cell line HT29 (HTB-38, ATCC) were grown in RPMI-1640 (31870025, Thermofisher Scientific), DMEM (11965092 ...
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bioRxiv - Cell Biology 2023Quote: NIH 3T3 immortalised mouse fibroblast and U2OS human osteosarcoma cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher), supplemented with 10% (v/v ...