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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... biotin anti-human IgA Fab (Invitrogen A24462) was added at a dilution of 1:100,000 for 2 hours at 4 C to detect human IgA ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... pseudokinase domain of mouse and human MLKL were expressed in Sf21 insect cells using the Bac-to-Bac system (ThermoFisher Scientific) and purified using established procedures28 ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pulsed with either 100µg/ml transferrin from human serum conjugated with Alexa Fluor™ 647(#T23366; Thermo Fisher Scientific) or vehicle (PBS ...
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bioRxiv - Bioengineering 2022Quote: The human monocyte cell line THP-1 was purchased from ATCC (TIB-202) and routinely cultured in RPMI 1640 medium (Life Technologies) containing 10% (v/v ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... Cells were re-suspended at 100µL/well with 1:1000 diluent with anti-human IgG-Alexa 488 (#A28175, ThermoFisher, Waltham, MA). Plates were incubated for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and cultured in a 10 cm dish containing irradiated MEFs in human embryonic stem cell (huES) medium containing DMEM-F12 (Life Technologies), knockout 10% serum replacement (Life Technologies) ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media (TH.Geyer, L0102) supplemented with 10% FBS (Thermo Fisher, 10500-064) and 100 U/ml Penicillin and 100 μg/ml Streptomycin (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2021Quote: ... at a concentration of 1×105 cells/100μl of complete medium with or without Human T-Activator CD3/CD28 Dynabeads® (ThermoFisher Scientific). NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes ...
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bioRxiv - Microbiology 2021Quote: ... and the human microglial cell line HMC3 (ATCC® CRL-3304™) were cultured in Dulbecco’s Modified Eagle Medium (10566016, Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells (authenticated by ATCC through their human STR profiling cell authentication service) were maintained in high glucose DMEM with Glutamax (Gibco 31966021), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Human Embryonic Kidney (HEK293T) cells were cultured in Dulbecco’s modified Eagle’s Medium–Glutamax medium (DMEM) (Gibco, Waltham, MA, USA, #6196-026) 10% FBS at 37°C and 5% CO2 with renewal of culture medium three times a week.
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid DNA construct was used to transfect Expi293F human cells using the Expi293F transfection kit and Expi293F expression medium (Thermo Fisher). Cells were harvested by centrifugation at 1,000 g for 30 min and resuspended in 1 x PBS supplemented with 30 mM imidazole ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Microbiology 2020Quote: ... A549 (human lung carcinoma) cells were used for infection experiments and were maintained in Ham’s F12 nutrient mixture (Thermo Fisher Scientific) with 8.3% FBS.
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bioRxiv - Molecular Biology 2021Quote: ... A549 cells (human, lung; CRM-CCL-185, ATCC) were cultivated in DMEM/F-12 medium with Nutrient Mix (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The human pancreas adenocarcinoma cell line Bx-PC3 obtained from ATCC (ATCC CRL-1687) was grown in RPMI-1640 medium (ThermoFisher Scientific) supplemented with 10% fetal calf serum at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Molecular Biology 2022Quote: ... Whitehead Institute Center for Human Stem Cell Research, Cambridge, MA) (Lengner et al., 2010) were maintained on MEFs in hESC media (DMEM/F12 [Thermo Fisher Scientific] supplemented with 15% fetal bovine serum [FBS] [Hyclone] ...
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bioRxiv - Physiology 2022Quote: ... ATCC) and human HEK-293T cells (catalog no. CRL-3216, ATCC) were grown in DMEM (catalog no. 31966-021, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (catalog no ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... The human anti-TNFα IgG1 monoclonal antibody was expressed in HEK-293F FreeStyle cell line expression system (Life Technologies, Carlsbad, CA) with co-transfection of vectors encoding p21 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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bioRxiv - Immunology 2022Quote: ... and human lung fibroblast (MRC-5 cells, ATCC® CCL-171™) were cultured in Minimum Essential Media (MEM, Life Technologies) supplemented with 4% FBS and 2 mM L-glutamine at 37°C in an atmosphere of 5% CO2.
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bioRxiv - Cell Biology 2022Quote: ... RPE1-hTERT (female, human epithelial cells immortalized with hTERT, Cat. No. CRL-4000) from ATCC were grown in DMEM (Life Technologies) or DMEM/F12 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... MEF dPS-/- (presenilin 1 and 2 knock out) and the human neuroblastoma SH-SY5Y cell lines (ATCC) were grown in DMEM/F12 medium (Thermofisher Scientific) and were supplemented with 10% of FBS ...
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bioRxiv - Microbiology 2021Quote: ... IgG opsonized cells were washed and incubated with 3μg/ml of an APC-conjugated anti-human total IgG secondary Ab (Invitrogen, catalog A21445) at 4°C for 25mins ...