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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Developmental Biology 2023Quote: ... Su(H) coding sequence was cloned from GH10914 (DGRC Stock 5973) into pAc5.1/V5-His A (Invitrogen) using the following primers ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... the crude DEL sample was loaded in Novex™ Hi-Density TBE Sample Buffer (5X, Invitrogen LC6678), and electrophoresis was run at 12 W constant power ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite software (v5.2.2 ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... PcPotD-cpsfVenus-E284 was synthesized (Integrated DNA Technologies) and cloned into the pBAD/His-B vector (Invitrogen) using Gibson assembly.
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bioRxiv - Cell Biology 2020Quote: The HaCaT cell line (Human adult low Calcium high Temperature) were grown in Dulbecco’s Modified Eagle Media (DMEM, Invitrogen) supplemented with 2 mM essential amino acids (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by performing a Ficoll-Paque gradient (Thermo Fisher) on whole blood ...
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bioRxiv - Molecular Biology 2021Quote: ... kidney) and HEK293T (Human embryonic kidney) cell lines were cultivated in Dulbecco’s modified Eagle (DMEM) (Thermo Scientific #12100-046) medium ...
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bioRxiv - Microbiology 2021Quote: Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were cultured in keratinocyte serum-free medium (KSFM) (Gibco) with 0.5 ng/mL human recombinant epidermal growth factor and 0.05 mg/mL bovine pituitary extract ...
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bioRxiv - Molecular Biology 2020Quote: Human female osteosarcoma U2OS and breast cancer MCF7 cells were cultured in DMEM + Glutamax (Thermo Fisher Scientific, 31966-047) supplemented with 10% FCS and 1% Penicillin/Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... The human monocytic THP-1 cell line was maintained in RPMI-1640 (made inhouse, RPMI 1640 powder (Life Technologies), 23.8mM Sodium Bicarbonate (NaHCO3 ...
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bioRxiv - Developmental Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of geltrex (Gibco, A14133-01), in E8 Basal Medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes) of human complete MSC medium (RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney (HEK)-293 cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11965-092) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... and MDA-MB-231 (ATCC HTB-26) human breast cancer cells were cultured in medium composed from standard Dulbecco’s Modified Eagle Medium (DMEM, Gibco), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... Human ACE2 ectodomain (NCBI Reference Sequence: NP_001358344.1, residues Q18-S740) was expressed in Expi293™ cells (Thermo Fisher Scientific) with a preceding murine IgG1 signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... Wild type/native human basigin (BSG-2, residues M1-L206) was expressed in Expi293™ cells (Thermo Fisher Scientific) with C-terminal rat CD4 domains 3+4 (CD4d3+4 ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed once with PBS and incubated with 1 ug/ml anti-human (Alexa Fluor 647; Invitrogen) secondary Abs and the viability dye AquaVivid (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of Geltrex (Gibco, A14133-01), in Essential 8 Basal Medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (hTERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK ...
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bioRxiv - Microbiology 2021Quote: ... USA) and Huh7.5 (human liver cell line) (kind gift from Dr. Craig Cameron) were cultured in DMEM (Life Technologies) containing 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Systems Biology 2020Quote: ... and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific). The primary antibodies included ...
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bioRxiv - Molecular Biology 2021Quote: ... and Caco-2 cells (human, male, colon; HTB-37, ATCC, RRID: CVCL_0025) were cultured in minimum essential medium (GIBCO) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and U-2 OS (female, human osteosarcoma epithelial) cells from ATCC were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies) supplemented with 10% v/v fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... and human foreskin fibroblast cells immortalized with human telomerase reverse transcriptase (HFF-hTERT cells)96 were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293T) cells were cultured in high glucose DMEM with 10% FBS and 1% Penicillin/Streptomycin (Gibco) in a 37°C incubator with 5% CO2 ...
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bioRxiv - Synthetic Biology 2022Quote: Codon-optimized DNAs encoding the variable library parts were codon optimized for expression in human cells using ThermoFisher GeneArt’s website tool and synthesized by ThermoFisher GeneArt ...
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bioRxiv - Biochemistry 2022Quote: Human Embryonic Kidney Expi293 cells were grown to a density of approximately 2×106 in Expi293 medium (Thermo Scientific). Following stress induction upon addition of 100 mM (final concentration ...
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bioRxiv - Cell Biology 2022Quote: Human mammary epithelial MCF-10A cells were cultured in basal Dulbecco’s Modified Eagle Medium (DMEM) (Gibco by Life Technologies) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Human mammary epithelial MCF-10A cells were cultured in basal Dulbecco’s Modified Eagle Medium (DMEM) (Gibco by Life Technologies) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... All media for human cell lines were supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco) and FBS (Peak Serum) ...
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bioRxiv - Microbiology 2020Quote: ... ATCC CCL-81) and human embryonic kidney cells (HEK293T; ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle media (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinomic lung epithelial (Calu-3) cells (ATCC, HTB-55) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Caco-2 and human skin fibroblast cells were grown in Dulbecco’s modified Eagles medium (DMEM) with Glutamax (Life Technologies) and supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... Human vaginal epithelium cells (hVECs, VK2/E6E7) were cultivated in Keratinocyte-Serum Free medium (Thermo Fisher Scientific, Massachusetts, USA) at 37°C in 5% CO2 as the suggestion by ATCC.
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bioRxiv - Immunology 2022Quote: ... Human monocyte-derived macrophages (MDMs) were derived from CD14+ peripheral blood monocytes by culturing cells in RPMI/1640 (Gibco) media containing 10% heat-inactivated human AB serum (Sigma-Aldrich ...