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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from nasopharyngeal swab samples (labeled as S2, S3, S4, S9) collected from SARS-COV-2 infected COVID-19 patients using TRIzol (Invitrogen) reagent following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S and RBD proteins were transiently expressed in Freestyle 293F system (ThermoFisher). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... extracted DMS-modified RNA from SARS-CoV-2 infected Vero cells was fragmented using the RNA Fragmentation kit (ThermoFisher Scientific). 1.5 μg of rRNA subtracted total RNA was fragmented at 70°C for 2.5 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: The codon-optimised full-length SARS-CoV-2 S gene was designed and purchased from GeneArt (Thermo Fisher Scientific, Poland). The gene was cloned into pCAGGS vector sequence verified that was a gift from Xingchuan Huang ...
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bioRxiv - Microbiology 2021Quote: ... As controls we used SARS-CoV-2-S-RBD pre-incubated only with buffer and non-specific mouse serum (Invitrogen). Plates were extensively washed with phosphate-buffered saline/0.05% Tween-20 (PBST) ...
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bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... BHK-21 were transfected to express the SARS-CoV-2 S protein using Lipofectamine 3000 (Thermo Fisher Scientific, Madrid, Spain) and after 24h transfected cells were inoculated with a replication-deficient rVSV-luc pseudotype (MOI ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Immunology 2021Quote: ... Each dilution was incubated at 37°C and 5% CO2 for 1 hour with an equal volume of 1000 plaque forming units/ml (PFU/ml) of SARS-CoV-2 (isolate USA-WA1/2020) diluted in DMEM (Gibco) containing 2% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 prefusion stabilized S trimer was incubated with yellow-green streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... were cultured in the presence of SARS-CoV-2-S (9pmol) (PepTivator) in 24-well plates and maintained in IMDM medium (Gibco) supplemented with penicillin (100 units/mL) ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 D614G S ectodomain was produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2020Quote: ... 250,000 or 1000 SARS-CoV-2 viral copies were used to start the initial cDNA synthesis by SuperScript III reverse transcriptase (Invitrogen), using a mix of random primers ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA Mini Kit (Invitrogen). Thereafter ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Cryopreserved PBMC from the SARS-CoV-2-infected participant and a SARS-naïve donor were thawed at 37°C and stained for SARS-CoV-2-specific memory B cells with a flow cytometry panel consisting of: a viability dye (7AAD, Invitrogen), CD14 PE-Cy5 ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Microbiology 2022Quote: ... total RNA from non-infected (Ctrl) or SARS-CoV-2 infected A549-hACE2 and hPCLS was isolated using Trizol (Invitrogen). RNA was treated with DNase (DNase-Free DNase Set ...
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bioRxiv - Microbiology 2022Quote: ... were transfected into Calu-3 cells using Dharmafect-4 transfection reagent (#cat: T-2004-02, Dharmacon) 48 hours prior to infection with SARS-CoV-2: siAAK1 (ID: s22494, Ambion), siBIKE (M-005071-01 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 targeting probes were custom-made #6007037-01 directed against the ORF1a sequences between positions 401-1327 (ThermoFisher). Subsequently ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 pseudovirus-based neutralization assay were performed in Nunc 96-well cell culture plates (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Neurons from K18-hACE2 and WT mice were inoculated with SARS-CoV-2 isolate USA-WA1/2020 at 30 MOI in 100 µL Neurobasal A (Invitrogen) for 8-well chamber slides and 200 µL for 24-well plates for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Six- to 12-week-old male and female mice were inoculated by the intranasal route with 103 pfu of SARS-CoV-2 in a volume of 50ul PBS (Gibco). Prior to inoculation mice were anesthetized by inhalation of isoflurane ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Immunology 2023Quote: Complement sufficient NHS (0-10% final concentration) was mixed 103 PFU of SARS-CoV-2 and RNAse A (1mg/ml,Thermofisher) for 2 hr at 37°C ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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bioRxiv - Immunology 2024Quote: ... Serum serial dilutions were incubated with 2000 PFU of an early SARS-CoV-2 isolate in 100 µl OptiPro medium supplemented with 1x GlutaMAX (both Gibco) for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...