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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with 2 µg pcDNA3.1 CoV-2 Spike using Lipofectamine2000 (Thermo Fisher). At 24 hours post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... Freshly-thawed SARS-CoV-2 was diluted in infection media (DMEM (Gibco) supplemented with 0.1% bovine serum albumin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... SARS-Cov-2 ORF1b FAM assay (PN 4332079, Life Technologies assay# APGZJKF) or spike (S ...
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bioRxiv - Microbiology 2021Quote: ... anti-SARS-CoV-2 (PA1-41098, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA), anti-PEDV (9191 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SARS-CoV-2 RBD were purified by nickel affinity columns (Invitrogen) while ACE2-Fc and antibodies were purified by protein A affinity columns (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-SARS-CoV-2 S antibody (PA5-81795, Invitrogen, 1:1000); rabbit anti-TDRD3 antibody (Bethyl Laboratory ...
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bioRxiv - Microbiology 2020Quote: ... anti-SARS CoV-2 nucleocapsid mouse monoclonal (1:10) (Thermo Fisher Scientific) and anti-ACE2 mouse monoclonal (1:20 ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with 2.5 µg of a SARS-CoV spike protein polyclonal antibody (PA1-41165, Thermo Fisher Scientific) for 30 min on ice before washing with 2.5 mL of FACS buffer and centrifuging at 1750 RPM for 7 min ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Microbiology 2020Quote: cDNA of SARS-CoV-2 was made using SuperScript IV Reverse Transcriptase (Invitrogen) following manufacturer’s instructions with modifications ...
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bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 stocks were titrated on Vero E6 cells using MEM (Gibco), 2% FCS (Labtech ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using Ion AmpliSeq SARS-CoV-2 Kit (Thermo Scientific, USA) following the manufacturer’s Protocol ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 S genes using Lipofectamine 3000 transfection reagent (ThermoFisher, CA)15 ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Microbiology 2022Quote: ... 1:5,000 dilution) (rabbit anti-Calreticulin, Cell Signaling, Cat #: 12238, 1:2,000 dilution) (mouse anti-Spike S2 or SARS-CoV Spike, Thermo Fisher, Cat #: MA5-35946, 1:2,000 dilution) (rabbit anti-anti-Spike S2 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Pathology 2020Quote: ... and SARS Spike protein (Thermo Fisher, 1:250), and with Phalloïdin-647 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 viruses were propagated in VeroE6 cells in Opti-MEM I (Invitrogen) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S RBD-serum mixtures were loaded onto MaxiSorp 96F plates (Nunc) coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Immunology 2022Quote: The SARS-CoV-2 VFLIP ectodomain (81) was produced in Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC-SARS-CoV-2-mCherry-2A was digested with BstBI (FastDigest, Thermo Fisher) and BamHI (FastDigest ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 S2 variants were transiently transfected in ExpiCHO-S cells (Thermo Fisher). CHO cells were maintained and transfected according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Microbiology 2022Quote: ... human Anti-SARS COV-2 Nucleocapsid monoclonal (E16C) (Thermo Fisher, MA1-7403, 0.1 mg/mL), was incubated as a 1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... to 1 µg/ml and mouse SARS-CoV-2 Nucleoprotein monoclonal antibody (MA1-7404, Thermofisher) to 10 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2020Quote: ... codon-optimized (for human cells) SARS-CoV-2 DNA (GeneArt Gene Synthesis, Thermo Fisher Scientific) based on the publicly available protein sequence in the National Center for Biotechnology Information database (NCBI Reference Sequence ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with mouse anti-SARS-CoV-2 N (#MA5-29981; Thermo Fisher Scientific) and anti-mouse IgG Alexa488 (#A-11029 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Validation and SARS-CoV-2 genome detection was performed with TaqMan detection systems (Applied Biosystems) based qPCR using SARS-CoV-2 specific primers and probes with the following sequences ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Immunology 2021Quote: ... RBD binding inhibition was measured using a SARS-CoV-2 Neutralizing Ab ELISA Kit (Invitrogen). For anti-Spike IgG experiments ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 RBD 6xHisTag constructs were expressed by transient transfection of Expi293F cells (Gibco) and purified using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 pseudoparticles were produced by co-transfecting HEK293T cells using Lipofectamine 2000 (Invitrogen) with plasmids encoding HIV-1 gag/pol ...
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bioRxiv - Pathology 2022Quote: ... and either pMD2.G or pTwist-EF1alpha-SARS-CoV-2-S-2xStrep using LTX (Invitrogen) transfection reagents according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 infected cells were washed with PBS and lysed with TRIzol reagent (Invitrogen). Total RNA was extracted using RNeasy mini kit (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of N501Y-SARS-CoV-2 BAC were transfected into Vero E6 (ATCC) with Lipofectamine 3000 (Invitrogen) in a 6-well plate according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: Viral RNA from SARS-CoV-2 infected HBEC cell lysates was extracted using TRIzol (Life Technologies) and purified using Direct-zol RNA MiniPrep Plus according to manufacturer’s instructions (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: ... complementary cDNA of SARS-CoV-2 clinical samples was generated using SuperScript II Reverse Transcriptase (Invitrogen), random primers and 10μL of RNA ...