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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Virus samples were serially diluted (10-fold starting at 1:10 initial dilution) in serum-free DMEM (recRSV-mKate and MA-SARS-CoV-2) and supplemented with 0.5% Trypsin (Gibco, recA/California/07/2009). Serial dilutions were added to cells seeded in 96-well plate at 1×104 cells per well ...
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bioRxiv - Immunology 2022Quote: ... The spike protein was biotinylated using EZ-Link NHS-PEG4-Biotin reagent (Thermo Scientific) as follows ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and SARS-CoV S protein were confirmed by transfection with the Turbofect transfection reagent (ThermoFisher) followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from SARS-CoV-2-infected Vero-E6 cells and purified with an Oligo (dT) kit (Thermo Scientific, Wilmington, DE, USA). The polyA purified RNA was fragmented and subjected to IP using an m6A-specific antibody ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Microbiology 2023Quote: ... mock and SARS-CoV-2 infected cultures were washed with calcium/magnesium-free PBS and disassociated using TrypLE (Gibco, 5 minutes at 37° C), after which samples were centrifuged (200 x g ...
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bioRxiv - Physiology 2022Quote: Five nanograms of total RNA were mixed (Supplementary Figure S1) with ERCC spike-in mix (Thermofisher Scientific, France) according to manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... LAIR-1 Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher), No-Weight Format (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... The ΔeOD-GT8 Fc was conjugated with Biotin (Thermo Fisher Scientific A30010) and then stained with BV605 (BioLegend 405229).
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific, Waltham, MA, USA) using primers and probes described previously8,9 (N2 set or E set).
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Genomics 2024Quote: ... and 2 μL of Dynabeads M-280 magnetic beads (Sheep Anti-Mouse IgG, Sheep Anti-Rabbit IgG, Invitrogen) using the SX-8G IP-Star Automated System (Diagenode) ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Systems Biology 2022Quote: ... ERCC synthetic spike-in 1 or 2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: ... ERCC synthetic spike-in 1 or 2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2×106 diluted ERCC spike-in RNAs (Ambion)] ...
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bioRxiv - Immunology 2021Quote: ... ACE2-Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific) at a molar ratio of 1:2 ...
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bioRxiv - Immunology 2020Quote: ACE2-Fc was biotinylated with EZ-Link NHS-PEG4-Biotin t (Thermo scientific) at a molar ratio of 1:2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The SARS-CoV-2 nucleocapsid gene was reverse transcribed and amplified using the TaqMan® RNA-to CT™1-Step Kit (ThermoFisher Scientific, Waltham, MA, USA), using Forward primers ...
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bioRxiv - Immunology 2022Quote: ... B.1.617.2 (delta) and B.1.1.529 (Omicron)] and MERS-CoV spike using Lipofectamine 2000 (ThermoFisher Scientific cat.# 11668019) to produce single-round infection-competent pseudoviruses (80) ...
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bioRxiv - Microbiology 2022Quote: ... Unbound biotin or SULFO-TAG was removed using Zeba spin desalting columns (ThermoFisher Scientific), and the incorporation ratio for each label was measured (See Dataverse file) ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV related coronaviruses) into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) (Naldini et al. ...
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bioRxiv - Microbiology 2020Quote: ... Drosten) and SARS-CoV (isolate HKU39849) were propagated on Vero cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (100 IU/mL ...
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bioRxiv - Immunology 2021Quote: ... The pool was incubated with biotinylated SARS CoV2 Spike extracellular domain bound to 5 μL streptavidin M280 dynabeads (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 Spike proteins (recombinant SARS-CoV-2 Spike Protein (SP-RBD, Arg319-Phe 541; cat# RP-87678, HEK293 cell expressed and binds ACE2) was obtained from Life Technologies Corporation ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and the MERS-CoV S protein with Avi-tag was conjugated to streptavidin-AF647 (Invitrogen cat.# S21374). After incubating the cells with Ab mixture for cell surface markers for 15 min in dark ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM Glutamax and 10% FCS (Invitrogen) in humid environment containing 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2% FCS (Thermo Fisher Scientific), 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation of Spike or RBD proteins was performed using the EZ-Link™ NHS-PEG4-Biotin (Life Technologies) using a 3-fold molar excess of reagent and using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... sheep anti-UMOD monoclonal antibody (R&D) was incubated with Dynabeads Biotin Binder (Thermo Fisher, Waltham, MA, CAT 11047). For each kidney isolate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen, Carlsbad, CA) followed by a donkey anti-rabbit IgG (H+L) ...
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bioRxiv - Microbiology 2021Quote: HEK293/TLR4 and HEK293/null cells (both Invitrogen) were maintained in 4.5 g/L glucose DMEM (Gibco ...