Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Leucine Rich Repeats And Immunoglobulin Like Domains Protein 3 LRIG3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... These gB domain sequences were cloned into pcDNA3.1(+) mammalian expression vector (Invitrogen) and transiently transfected into Expi293F™ cells for five days ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies to viral proteins were directly conjugated using antibody labeling kits (ThermoFisher). For viability staining ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
-
bioRxiv - Biochemistry 2022Quote: ... followed by secondary AlexaFluor 647 goat anti-mouse immunoglobulin G (1:1,000, Invitrogen A-21235) with Hoechst 33342 dye (1:3,000 ...
-
bioRxiv - Microbiology 2019Quote: ... The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies) in a total volume of 50 µl ...
-
bioRxiv - Immunology 2020Quote: ... Immunoglobulin epsilon (ε) heavy chain transcripts were amplified (StepOnePlus Real-Time PCR System, Applied Biosystems) using custom TaqMan gene expression kits (Applied Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... and 1:1,000 for AlexaFluor 488- or 594-labeled donkey anti-mouse immunoglobulin (ThermoFisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... RNA ladders were obtained by alkaline hydrolysis of the CRISPR repeat RNA (Thermo Fisher Scientific, RNA Protocols).
-
bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
-
bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
-
Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... HRP conjugated rec-Protein G antibodies (Invitrogen, 101223) were diluted (diluent mixture described above ...
-
bioRxiv - Cell Biology 2019Quote: ... antibodies coupled to Protein G-Dynabeads (ThermoFisher Scientific) for 1 hour at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... antibodies were coupled to Dynabeads Protein G (Invitrogen) and incubated overnight at 4°C with 10-fold diluted chromatin ...
-
bioRxiv - Microbiology 2019Quote: ... antibody was crosslinked to DynabeadsTM protein G (Invitrogen) beads using 5 mM BS3 crosslinker (CovaChem ...
-
bioRxiv - Cell Biology 2020Quote: Anti-MAP2 antibody (microtubule associated protein 2; Thermofisher) was used to identify neurons ...
-
bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
-
bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
-
bioRxiv - Plant Biology 2019Quote: ... About 20 µg of total soluble proteins of each sample was loaded to 3-12% Bis-Tris 1.0 mm Invitrogen NativePAGETM protein gels from ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... antibodies were 3-fold serially diluted in MEM medium (Gibco) from 20 μg/mL for preparing the working solution ...
-
bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800 ...
-
bioRxiv - Immunology 2023Quote: ... antibodies for 3 hours and stained for TMRM (Thermo Fisher) and active-Caspase3 (BD Biosciences) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and Cyanine 3-conjugated anti-mouse IgG antibody (M30010, Invitrogen). Embryos were washed with PBS for three times and then PBS was replaced with 10% ...
-
bioRxiv - Biochemistry 2022Quote: ... The antibody-protein complexes were isolated by incubation with magnetic beads (Invitrogen Dynabeads protein A and protein G) for 6 hours at 4℃ ...
-
bioRxiv - Cancer Biology 2019Quote: ... A PCR for CG-rich sequences was performed on 50ng DNA using the PCRX Enhancer System (Thermo Fisher Scientific), containing 10X PCRX amplification buffer ...
-
bioRxiv - Microbiology 2022Quote: ... all culture being conducted using a commercially-prepared serum-free medium supplemented with lipid-rich bovine serum albumen (Gibco RPMI with 0.5% AlbuMAX™ II lot number 2177881) ...
-
bioRxiv - Cell Biology 2023Quote: ... yeast cells were grown in rich medium (YPD) containing 20 g/l peptone (Gibco™ Bacto™ BD Biosciences), 10 g/l yeast extract (Bacto™ BD Biosciences ...
-
bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used as primary antibodies: FFAR 3 (1: 100, #PA5-97745, Invitrogen) and GPR 43(1 ...
-
bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
-
bioRxiv - Molecular Biology 2020Quote: Proteins of interest were precipitated using antibodies pre-conjugated to Dynabeads Protein A (Invitrogen). To this end ...
-
bioRxiv - Neuroscience 2023Quote: ... antibody-bound proteins were coupled to Dynabeads Protein G beads (30µL, Thermo Fisher Scientific) by gentle agitation for 1.5h at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibody-protein complexes were pulled down using 50 µL of protein-G Dynabeads (Invitrogen). Beads were washed 3 times with 300 μL of PBS supplemented with 0.02% Tween-20.
-
bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
-
bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
-
bioRxiv - Biochemistry 2021Quote: ... with 50ug of protein separated using 3-8% tris-acetate gels (Invitrogen EA0378) and transferred using an iBlot2 transfer system (Invitrogen IB21001) ...
-
bioRxiv - Biophysics 2021Quote: ... and loaded into NativePAGE™ 3–12% Bis-Tris protein gels (ThermoFisher Scientific). BN-PAGE was performed using two different running buffers ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein complexes were separated on 3-12% NativePAGE Novex Bis-Tris gels (Invitrogen). Electrophoresis was performed for 30 min at 150 V followed by 2 hours at 250 V ...
-
bioRxiv - Biochemistry 2023Quote: ... Native PAGE™ Novex® 3-12% Bis- Tris Protein Gels (Thermo Fisher) gels were loaded with 40 μg of mitochondrial proteins ...
-
bioRxiv - Microbiology 2023Quote: ... for LapA or 3 ul Spectra Multicolor High Range Protein Ladder (Thermo Fisher) for MapA ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
-
bioRxiv - Immunology 2020Quote: ... and purified using Nickel-NTA resin for gB Domain II (Thermo Fisher Scientific), and lectin resin (VWR ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell line identities were verified by short tandem repeat genetic profiling (AmpFISTR Identifiler PCR Amplification Kit; Applied Biosystems), routinely tested for mycoplasma (Mycoplasmacheck ...
-
bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
-
Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
-
bioRxiv - Neuroscience 2023Quote: ... Lysates containing 60ug of total protein were electrophoresed for 3 hrs at 40C and 100 V using 3%-12% Bis-Tris polyacrylamide gels (ThermoFisher, BN1001BOX) and a running buffer containing 15mM Bis-Tris ...
-
bioRxiv - Physiology 2019Quote: ... or Alexa Fluor 488-conjugated cross-adsorbed goat anti-rabbit immunoglobulin G (Life Technologies, Burlington, ON) diluted 1:200 in DPBS containing 10% NSS ...
-
bioRxiv - Microbiology 2021Quote: ... pneumococcal-specific Abs were detected using horseradish peroxidase–conjugated goat anti-mouse immunoglobulin M (IgM; Invitrogen), IgG (Millipore Sigma) ...
-
bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 555 donkey anti-rabbit immunoglobulin G (1:200, Thermo Fisher Scientific; A-31572).