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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... by immunoprecipitation in antibody (each 20 µg) immobilized agarose beads (each 50 µl) from Pierce™ Co-Immunoprecipitation Kit (Thermo Scientific™). For each assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Single-stranded cDNA was synthesized from 1 μg RNA in a 20 μl final volume using the High-Capacity cDNA Reverse Transcription Kit with RNase inhibitor (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 150 ng of RNA in a 20 μl reaction using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4368814). qPCR was performed using TaqMan Fast Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was prepared with 2 μg of the treated RNA in 20 μL total volume of reaction mix using a high-capacity cDNA reverse transcription kit from Applied Biosystems (4368814). The cDNA was further diluted 1:10 to obtain a final concentration of 10 ng/μL ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated and cDNA was generated using 1-μg of DNase I-treated RNA using a Superscript III first strand synthesis system kit and oligo(dT)20 primer (Thermo Fisher Scientific). Samples were then treated with RNase H (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: First-strand cDNA synthesis was performed with total RNA isolated from 20 eleuthero embryos per replicate with a Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific, USA) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were digested for 20 h at 37 °C and then labeled with the TMTsixplex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of RNA was input into each 20 µl cDNA reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #4368814) under cycling conditions 25°C 10 mins ...
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bioRxiv - Microbiology 2023Quote: ... and up to 2 µg of RNA was used per 20 µl cDNA synthesis reaction (High-Capacity cDNA Reverse Transcription Kit, Thermo Fisher Scientific). 10 µl RT-qPCR reactions consisting of 2 µl cDNA ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min or with a mix of SYTO 9 (3.34 µM) and propidium iodide (PI, 20 µM) from the LIVE/DEAD BacLight Bacterial Viability kit (#L7012, Molecular Probes, Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Immunology 2024Quote: ... The precipitated DNA was resuspended in 20 µL of RNase-free H2O and in vitro transcribed with an SP6 mMessage mMachine In Vitro Transcription Kit (ThermoFisher, cat. AM1340). The generated RNA was electroporated into 1.2 × 107 BHK-21 cells ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were centrifuged at 15,000 x g at 4°C for 20 min and protein concentration was quantified using bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). 10-25 µg of proteins were resolved in 8% SDS-PAGE gels before being transferred onto polyvinylidene (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... probenecid (1:250) and PowerLoad concentrate (1:100) (Fluo-4 Calcium Imaging Kit, Molecular Probes) in BrainPhys basal media for 30 min (37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted LSK cells were cultured in 48-well-plates for 48 hours in a 1:1 ratio of Stem Pro-34 SFM (Gibco/ Thermo Scientific, Waltham, MA, USA) and Iscoves modified Dulbecco medium (IMDM ...
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bioRxiv - Microbiology 2022Quote: ... and Taqman Gene Expression Assay kits (Invitrogen; Suppl. Table 1) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Tests included 1) the Purelink Microbiome DNA Purification Kit (Thermofisher), which is optimized for microorganism lysis ...
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bioRxiv - Cancer Biology 2024Quote: ... and mixed with 1:50 B:A reagent (Thermofisher BCA Kit) to determine the cell lysate concentration in each sample ...
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bioRxiv - Neuroscience 2023Quote: ... Qubit 1 × dsDNA HS Assay kit (#Q33230, Thermo Fisher Scientific) was used to quantify libraries ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Immunology 2022Quote: Cell culture supernatants from BMDCs were analyzed by ELISA for IL-12p70 (R&D) and IFNβ (Invitrogen). Plates were read using a Tecan Sunrise microplate reader at 450 nm ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were validated using PCR and western blot and screened for steroidogenic induction using aldosterone ELISAs (Invitrogen).
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2021Quote: ... IL1β and IL6 were quantified using a specific rat enzyme-linked immunosorbent assay (ELISA, Invitrogen, Thermo Scientific) following the manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... IL1β and IL6 were quantified using a specific rat enzyme-linked immunosorbent assay (ELISA, Invitrogen, Thermo Scientific) following the manufacturer protocol.
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bioRxiv - Pathology 2024Quote: ... and CXCL1 levels were measured in the supernatant of human precision-cut liver slices using ELISA (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... ELISA were performed as previously described and bound phage were detected with TMB substrate (Thermo Fisher Scientific) following a 30-min incubation with HRP-conjugated anti-M13 monoclonal antibody (Antibody Design Laboratories)19 ...
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bioRxiv - Biochemistry 2023Quote: ... assay was developed by incubating cells with 200 μl of TMB-ELISA (Thermo Scientific, Cat no: 34028) until the light blue color appeared ...
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bioRxiv - Neuroscience 2023Quote: ... Optical density was measured by 450nm light with an ELISA microplate reader (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Immunology 2023Quote: ... washed 5 times with PBS-T and incubated with 1x TMB ELISA substrate solution (Thermo Fisher Scientific). The reaction was stopped with 2 N H2SO4 ...
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bioRxiv - Biophysics 2023Quote: ... left to incubate for 16 hours and the lysate is recovered for tPA determination by Elisa (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... IL-6 was analyzed from blood serums by sandwich enzyme-linked immunosorbent assay (ELISA; Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA following manufacturer’s instructions (Thermo Fisher Scientific #88808688).
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bioRxiv - Microbiology 2020Quote: ... The amplified DNA from one 96-well plate was normalized using a SequalPrep Normalization Plate (Invitrogen), and pooled into a single library ...
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bioRxiv - Bioengineering 2020Quote: ... or rectangular uniwell plates with overall dimensions of 96-well plates (Thermo Fisher Scientific, Waltham, MA) using custom-written software (see also below ...
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bioRxiv - Immunology 2022Quote: HLA-G+ EVTs were seeded on fibronectin-coated tissue culture plates (Nunc Cell-Culture Plates, ThermoFisher) at a density of 1 million cells/ml EVT culture medium ...
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bioRxiv - Molecular Biology 2020Quote: ... The assay was performed on 96-well plates (MicroAmp Fast 96-Well Reaction Plate, Applied Biosystems), in a total volume of 25 μL for each reaction ...
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bioRxiv - Immunology 2022Quote: HLA-G+ EVTs were seeded on fibronectin-coated tissue culture plates (Nunc Cell-Culture Plates, ThermoFisher) at a density of 1 million cells/ml EVT culture medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were prepared as follows in 384 well plates (MicroAmp Optical 184-Well Reaction Plate, Thermofisher): 5 µL Luna one-step reaction mix ...
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bioRxiv - Microbiology 2023Quote: ... with a collection plate (deep well storage plate, 96-well, 2,2 mL; Thermo Fisher Scientific, Germany).
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bioRxiv - Bioengineering 2022Quote: ... prior to removal of DNA template by addition of 1 μl DNase (Ambion MEGAscript kit, Durascribe kit) per 20 μl IVT reaction mixture ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies were rat α-Kit (1:500, Rat α-Kit clone ACK4, ThermoFisher, product MA5-17836), mouse α-calbindin (1:2000 ...
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... 20 μl IP eluate was separated by 4-20% SDS-PAGE and stained with GelCode Blue (Thermo Scientific). Each lane was cut into 15 pieces ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 WT and 20 s741 mutant larvae were incubated with 6.4 μM BODIPY FL C5 (Invitrogen, Waltham, MA) that was suspended in an egg yolk feed at room temperature with agitation for 7 hours ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 20 min at 37°C with tetramethylrhodamine-ethyl-esther (TMRE, 20 nM, Thermo Fisher) and MitoTrackerGreen (MTG ...
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bioRxiv - Cell Biology 2022Quote: ... 10-20 μg of each lysate was loaded onto the 4-20% Tris-Glycine gel (Thermo Fisher Scientific) or 4-12% NuPAGE Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 2min at 85C and loaded on a TrisGlycine 4-20% SDS-PAGE gel (4-20%, Invitrogen) along with a PrecisionPlus Kaleidoscope protein ladder (BioRad 1610375) ...
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bioRxiv - Microbiology 2020Quote: Stock solutions were diluted in DMSO and 2µL of the resulting solutions plus 148µL of a bacterial suspension standardized to an OD600 of ∼ 0.1 and diluted 1:500 in 10:90 was added to a 96 well plate (Nunc) in triplicate ...