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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and 20 IU/mL penicillin plus 20 mg/mL streptomycin) and digested with 0.025% trypsin-EDTA (Gibco) for 8 min at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cells were treated with 20 μM Niraparib or 20 μM PARG inhibitor in FluoroBrite medium (ThermoFisher) supplemented with 10% FBS for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts (20 µg) were separated on a Tris-Glycine 4-20% gradient precast polyacrylamide gel (Invitrogen), and transferred to 0.22 µm pore nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Control samples were incubated in 20 μl of TMG(50) plus 20 ng of RNase A (Invitrogen) for 10 min at 30 °C prior to the telomerase assay ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... diluted first in 20 μl of Pluronic™ F-127 (20% w/v in DMSO, P3000MP, Invitrogen), and subsequently in 80 μl of PBS ...
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bioRxiv - Cell Biology 2024Quote: Cells were treated for 25 minutes with 20 µM EdU or 20 µM OPP (Thermo Fisher C10456). Following this pulse ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Genomics 2020Quote: ... Cells were sorted with a BD Influx Sorter into 96-well plates and lysed using the Arcturus PicoPure Kit (Applied Biosystems) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit) for 1h and spread on Luria–Bertani agar plates (245×245 mm, Thermo Fisher Scientific) containing 100 µg/mL ampicillin ...
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bioRxiv - Microbiology 2022Quote: HK1 grown in 100 mm plates were transfected with 5 μg of RFP-LMP1 or GFP-PD-L1 plasmid using Lipofectamine 3000 transfection kit (Invitrogen, L3000015). Lipofectamine reagents were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Total protein content was determined by the Lowry method (77) adapted to a 96-well plate assay with the Microplate BCA Protein Assay Kit (23252, Thermo Scientific), using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted in 96-well PCR plates in 9 µl of RT/pre-amp mix from the CellsDirect One-Step qRT-PCR Kit (Life Technologies) and were kept at −80°C at least overnight ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
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bioRxiv - Microbiology 2019Quote: ... The DNA amplicons in each PCR reaction was normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001). The normalized reactions were pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Cell Biology 2019Quote: ... The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... Samples were quantified in a 96-well plate reader using the Quant-IT DNA high sensitivity assay kit (Invitrogen, Cat #Q33232) according to the manufacturer’s protocol and pooled into 1 tube ...
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bioRxiv - Immunology 2020Quote: ... or luminal cells collected in vaginal washes were plated in 96-well plates and incubated with Live/Dead Fixable Aqua Dead Cell Stain kit (Molecular Probes) for 15 minutes at room temperature (RT ...
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bioRxiv - Genomics 2021Quote: Protein lysates derived from same plates used for metabolomics collection were assayed for total protein concentration with Pierce BCA Protein Assay Kit (ThermoFisher 23225) for normalizing raw metabolite values ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated into black 96-well clear bottom plates and analyzed using CyQuant cell proliferation assay kits (Thermo Scientific, c7026).
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bioRxiv - Immunology 2022Quote: ... Amplified linear DNA cassettes encoding monoclonal heavy and light chain genes were co-transfected into Expi293 cells in 96-well deep-well plates using the Expi293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... A volume of 200 μL of milk samples were used as starting material for magneticbased DNA extraction using a 96-well plate and CORE kit in a KingFisher instrument (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted from 8-μm snap frozen sections of distal femur and proximal tibial growth plates in Axin2CreERT2;R26RTdTomato mice using the Picopure RNA Isolation Kit (KIT0204, Thermo Fisher) as previously described.56 Microdissections of resting chondrocytes and proliferative chondrocytes were performed using a Leica LMD 7000 laser microdissection system (Leica Microsystems) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 96 well plates (6055302, Revvity) and stained with Blue/Green ReadyProbes Cell Viability Imaging Kit (Invitrogen, R37609) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted from a single well of a 6-well plate using a mixture of RIPA lysis buffer and protease inhibitor then quantified using Pierce BCA kit (ThermoFisher Scientific), were separated via SDS-PAGE and subsequently transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilised in ice-cold 100% methanol for 10 minutes at -20°C and labelled using an Alexa Fluor™ 647 HCS assay kit (ThermoFisher Scientific). Cells were blocked in 5% normal goat serum and 2% BSA for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...
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bioRxiv - Bioengineering 2021Quote: ... RNA samples that were not degraded were converted to cDNA in 20 μL reactions using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed from oligo(dT)20 primers (Integrated DNA Technologies) using the SuperScript III first strand synthesis kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... pooled from 20-24 jellyfish was extracted using the RNAqueous-Micro kit according to the manufacturer’s instructions (Ambion, Invitrogen, Thermo Fisher Scientific, #AM1931). Two biological replicates were generated for each sample type ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer cells were fixed in 4% PFA for 20 min at RT and stained according to the Click-iT EdU imaging kit protocol (Invitrogen, California, USA). Stained cancer cells were imaged and quantified using the ImageXpress Micro Confocal microscope (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from 0.5-1 g of total RNA in a 20 μL volume using a High-Capacity cDNA Reverse Transcription Kit (Cat#: 4368814, Applied Biosystem, Thermo Fisher Scientific). 5 minutes at 25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA by reverse transcription was performed in 20 μL reactions with 200 ng of total RNA with the High-Capacity cDNA reverse transcription kit (Applied Biosystems, USA). For the subsequent PCRs ...
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bioRxiv - Immunology 2021Quote: ... DNase-I-treated total RNAs were diluted 10-fold into cDNA synthesis reactions (20 μL final volume) with or without reverse transcriptase using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher™). For ddPCR (gDNA or cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... M or BS samples were captured into separate caps of 0.5 mL micro-tubes filled with 20 uL of extraction buffer (Arcturus PicoPure RNA isolation kit, ThermoFisher Scientific; MA, US).
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of RNA from each sample were reverse transcribed in 20 μl to produce cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... and Atxn2Q100/+ cohorts were concentrated from 80 µL to 20 µL using the GeneJET Purification Kit (Thermo Fisher Scientific, cat no. K0702). Two minimal modifications to the manufacturer’s protocol were made ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 flow cell from each condition was removed from the system and the bacterial cells were stained with 300 µl of 3.34 µM SYTO9 and 20 µM propidium iodide (PI) (LIVE/DEAD™ BacLight™ Bacterial Viability Kit, Molecular Probes) for 15 min for the observation of live and dead cells respectively using confocal laser scanning microscopy ...
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bioRxiv - Genomics 2021Quote: ... RNA samples were rigorously treated with DNase for 20 min at 37 °C using the TURBO DNA-freeTM Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™). 5 μl of the resulting cDNA was used for a final volume of 20 μl PCR with indicated oligo primers ...
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bioRxiv - Genetics 2021Quote: ... Purified RNA was diluted to 20 ng/μl and used to produce cDNA pools with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). RT-PCR was conducted using the Q5 High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... primers and 20 ng of cDNA were mixed in a 10-µl template and amplified using an SYBR Green Supermix kit (Thermo Fisher Scientific) with a Light Cycler 480 II instrument (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 μg of the total RNA was used for cDNA synthesis within 20 μL reaction mixtures according to the protocol provided by a reverse transcription kit (Thermo Fisher Scientific). When the reaction finished ...