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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1×105 cells of Vero E6 cells were seeded in 24-well plate (Thermo Fisher Scientific, MA, USA). Cells with approximately 80–90% confluency were transfected with 1 μg of IVT ChulaCov19 using Lipofectamine™ MessengerMax™ (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Products were resolved by spotting 1 μL of quenched reactions onto polyethyleneimine (PEI) TLC plates (Fisher Scientific M1055790001). TLC plates were developed in 0.3 M potassium phosphate ...
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bioRxiv - Microbiology 2022Quote: ... the plates were developed using 50 μL of 1-Step Ultra TMB (3,3’,5,5’-tetramethylbenzidine; Thermo Fisher Scientific), and the reaction was stopped with an equal volume of 2 M sulfuric acid before the optical density (OD ...
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bioRxiv - Cell Biology 2022Quote: ... worms were transferred to bacteria-seeded plates containing 1 mM indole-3-acetic acid (Acros Organics, Cat #122160250) and incubated for the indicated time periods before analysis.
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bioRxiv - Microbiology 2024Quote: ... 1 in 10 serial dilutions solutions were created using sterile PBS in a 96 well plate (ThermoFisher Scientific). Bacterial counts (CFU/mL ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed 4 times with wash buffer before being developed with 1-Step PNPP substrate (ThermoFisher) for ∼ 30 minutes ...
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bioRxiv - Genetics 2023Quote: Single colonies picked from plates were inoculated and cultured in YPD (1% yeast extract (LP0021B, Oxoid, Thermo Scientific), 2% peptone (LP0037B ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 5,000 LECs were first seeded on a U-bottom 96-well plate pre-coated with 1% agarose (Invitrogen) o/n to let the LECs form spheroids under non-adherent conditions ...
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bioRxiv - Developmental Biology 2023Quote: Organoids were transfered 1 organoid per well in 24-well plates (Non-treated) (144530 Thermo Fisher / Life Technologies) pre-coated as follows ...
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bioRxiv - Developmental Biology 2023Quote: Organoids were transfered 1 organoid per well in 24-well plates (Non-treated) (144530 Thermo Fisher / Life Technologies) pre-coated as follows ...
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bioRxiv - Genomics 2023Quote: ... Cells on remaining plates were washed with PBS and fixed with 1% methanol-free formaldehyde (Thermo Scientific 28908) in DMEM at room temperature for 8 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 1 million live cells per sample were transferred to a 96-well conincal bottom plate (Thermo Scientific, 249570) and stained.
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Immunology 2021Quote: ... The plates were read at 450 nm on a VarioSkan Plate Reader (Thermo Fisher Scientific) within 10 minutes of adding stop solution.
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bioRxiv - Immunology 2021Quote: ... The plates were read at 450 nm on a VarioSkan Plate Reader (Thermo Fisher Scientific) within 10 minutes of adding stop solution.
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bioRxiv - Microbiology 2019Quote: ... Ninety-six-well plates (MicroAmp EnduratePlate Optical 96-well fast clear reaction plates; Applied Biosystems) were used and heated from 25 to 95°C with a ramp rate of 0.05°C/s and read by a thermocycler (QuantumStudio 5 real-time PCR system ...
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bioRxiv - Molecular Biology 2020Quote: ... Each well of the microtiter plate (MicroAmp fast optical 96-well reaction plate, Applied Biosystems) was filled with 20 µL of final solution ...
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bioRxiv - Neuroscience 2022Quote: Plates were transported from plate hotels using a CRS CataLyst Express robot from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed every hour using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 620 nm.
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bioRxiv - Neuroscience 2022Quote: Assay plates (Nunc™ MaxiSorp™ 96-well polystyrene plate catalog #449824; Thermo Fisher Scientific) were coated with 100 μL/well of the capture antibody (2B7 or anti-PP2A ...
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bioRxiv - Microbiology 2023Quote: ... Cells were cultured in 96-well plates using a spectrophotometer plate reader (Thermo Fisher Scientific) with antibiotic concentrations ranging from 0.25 μg/mL to 2048 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... The plate absorbance was measured by a plate reader at 450nm (Multiscan Sky, Thermo Scientific). The number of epitopes was calculated by comparison with a calibration line obtained using rabbit red blood cells [27].
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bioRxiv - Bioengineering 2023Quote: ... The plate absorbance was measured by a plate reader at 450nm (Multiscan Sky, Thermo Scientific).
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bioRxiv - Plant Biology 2023Quote: After coating of the 96-well plates (Nunc-Immuno® Plates, Thermo Scientific, Waltham, USA) with 100 µl per well of the sample in triplicate in different concentrations in ddH2O (12.5 µg mL-1 for AmOx and KOH ...
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bioRxiv - Immunology 2024Quote: ... Antigen-coated plates were centrifuged briefly at approximately 2,000xg in a plate spinner (Fisher Scientific) to ensure even coating then incubated on a rocker at 4°C (minimum 4 hours ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run using primer/probes at 1:20 dilution and Taqman FastVirus one-step reaction mix (Thermo Scientific), and amplified using an Applied Biosystems QS3 cycler ...
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bioRxiv - Genetics 2021Quote: ... For cDNA synthesis 5 μg of total DNase-treated RNA was reverse transcribed with 1 μL of oligo-(dT)20 primers (50 μM) and 0.25 μL of SuperscriptIII (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA from the samples was synthesized using 0.5-1 μg of total RNA in a 20 μl reaction with Superscript VILO master mix (Invitrogen) following the manufacturers’ recommendation ...
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm thick sections were then collected and immunostained with Streptavidin Alexa Fluor 405 (1:200; Thermo Fisher: S32351) and rat anti-PLVAP (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (∼1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
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bioRxiv - Immunology 2021Quote: ... 0.1% Tween-20) and incubated for 1 h with HRP-conjugated mouse or rabbit secondary antibody solutions (Thermo Fisher) diluted in 5% milk (diluted 1/10000) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Virus titres were measured from a thawed aliquot by: (1) mixing 20 μl with 200 μl of TRIzol (Invitrogen); (2 ...
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bioRxiv - Molecular Biology 2019Quote: ... a 20 µL reaction was setup by adding 1 µL each of oligodT (50 µM, Invitrogen, cat No. 18418020) and dNTP mix (10 mM ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified using two rounds of centrifugation (20,000 X g, 20 min) and then bound to 1 ml of Ni-NTA agarose beads (Invitrogen) in lysis buffer containing 5mM imidazole for 2-4 hr ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... saturated circles were cut out of the Whatman FTA cards and incubated in 800 uL TE buffer (10 mM Tris-Cl, 1 mM EDTA, pH 8.0) with 20 uL Proteinase K (Invitrogen) for 2 hours at 65°C ...
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bioRxiv - Bioengineering 2020Quote: ... and stained for 20 minutes on ice with 1 µg/ml Alexa Fluor 647-conjugated streptavidin (Thermofisher Scientific S21374). After two more washes with HyClone PBS (without BSA ...
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bioRxiv - Bioengineering 2020Quote: Growth medium (high-serum medium - 79 % Hams F-10 from Gibco, 20 % fetal bovine serum, 1 % antibiotic-antimycotic Gibco, with 2.5 ng/mL recombinant human fibroblast growth factor R&D Systems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Biochemistry 2020Quote: 100 ml of cell culture was treated with 1% formaldehyde and 20 mM disuccinimidyl glutarate (DSG) (20593; ThermoFisher Scientific) in 1X PBS with constant shaking for 20 minutes at 25°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... stained for 20 min in SYBR™ Green II RNA Gel Stain (1:10,000 in 0.5x TBE, ThermoFisher Scientific) and imaged on the iBright™ (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Plant Biology 2020Quote: ... RNA was precipitated from 200 μL of the supernatant with 1 μL 20 mg/mL glycogen (Thermo Fisher Scientific), 20 μL 8 M LiCl ...
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bioRxiv - Microbiology 2020Quote: ... The concentrations and purity of 1:20 dilutions of dsRNA were measured with a NanoDrop 1000 (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 1:200 anti-p80 antibody (H161) (Developmental studies hyrbidoma bank) in PBS/0.1 % Tween 20 (Fisher Scientific, Pittsburgh, PA) (PBST ...