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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the media in each plate was replaced with 40 μL of 1× Hank’s Balanced Salt Solution (HBSS) containing 20 mM HEPES pH 7.4 and 4× dilution of Fluo4 (Thermo Fisher). The assay plate was incubated at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... The testes were then washed with two portions of PBST and incubated in PBSTX (0.1% Tween-20 and 0.3% Triton X-100 in 1×PBS) with 5% normal goat serum (Life Technologies) at room temperature for at least 1 h ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (ThermoFisher, #15437270). After electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized with 0.5% Triton X-100 (in PBS) followed by Phalloidin 594 (1:20 dilution in PBS; A12381, Invitrogen) staining for 1 hr at room temperature.
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bioRxiv - Neuroscience 2021Quote: ... 500 μl of washing buffer was added (Tris-HCL 10 mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0.04 U/μL ...
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bioRxiv - Biochemistry 2020Quote: ... beads were washed three times with 0.5-1 ml of SA-RNP lysis buffer (20 mM Tris-HCl (pH 7.5, Ambion, AM9850G, and Ambion, AM9855G), 150 mM NaCl (Ambion ...
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bioRxiv - Immunology 2022Quote: ... 0.1% Tween-20) and incubated for 1 hour with HRP-conjugated mouse or rabbit secondary antibody solutions (Thermo Fisher) diluted in 5% milk (diluted 1/10000) ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries in the flowthrough were precipitated at −20 °C overnight in ethanol with 0.3 M sodium acetate and 1 µl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on a Tapestation (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM β-mercaptoethanol [BME]) in two steps using Slide-A-Lyzer G2 (20 kDa cutoff; Thermo Fisher Scientific), with 60 μg of Tobacco etch virus protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was precipitated from 400 μL of the supernatant with 1 μL 20 mg/mL glycogen (Thermo Fisher Scientific), 40 μL 8 M LiCl ...
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bioRxiv - Bioengineering 2019Quote: ... The myotubes were washed 3 times using PBS containing Tween-20 (0.1% v/v) and incubated with AlexaFluor 488-conjugated goat anti-mouse IgG (1:250; ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Genetics 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (~1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Neuroscience 2019Quote: ... iBBB monolayers were then washed twice in TBS (1X) with 0.025% Tween 20 and mounted with Gelvatol containing Hoescht (1:1000; Invitrogen) for nuclear staining ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 20 min at room temperature with SYTO RNASelect green fluorescent dye in PBS (1:10.000, S-32703, Invitrogen). Samples were washed with PBS and mounted with ProLong Gold antifade reagent ...
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bioRxiv - Microbiology 2019Quote: ... The quantitative PCR was performed in 20 μL reaction mix containing 1× TaqMan Gene Expression Master Mix (Applied Biosystems), 900 nM each primer ...
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bioRxiv - Biochemistry 2021Quote: ... Extracts were clarified using two rounds of centrifugation (20,000 X g, 20 min) and then bound to 1 ml of Ni-NTA agarose beads (Invitrogen) in lysis buffer containing 5mM imidazole for 2-4 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Caco2 and MDCK cells were routinely grown in DMEM 4.5 g/l glucose supplemented with 20% (Caco2 cells) or 10% fetal bovine serum and 1% penicillin-streptomycin (Gibco, Thermo Fischer Scientific ...
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bioRxiv - Immunology 2020Quote: ... 0.1% Tween-20) and incubated for 1 hour with HPR-conjugated mouse or rabbit secondary antibody solutions (Thermo Fisher) diluted in 5% milk (diluted 1/10000) ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended at 1 x 106 cells per mL in amastigote medium (Schneider’s Drosophila medium [Gibco], 20% FBS [Gibco] and 15 µg mL-1 HEMIN [Sigma] ...
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bioRxiv - Microbiology 2021Quote: ... cells persistently infected with BoDV-1 strain huP2br 20 were cultured in high-glucose (4.5%) Dulbecco’s modified Eagle’s medium (DMEM) (11965092; Thermo Fisher Scientific) supplemented with 5% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... Three 5-minute washes with block (1% BSA with 0.1% Tween-20) were done prior to incubation in secondary antibodies (ThermoFisher Alexafluor goat anti-mouse 488 and Alexafluor goat anti-rat 594 ...
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bioRxiv - Genomics 2022Quote: ... supernatant was fully removed and cells were resuspended in 50 uL of CLB (Nuclei buffer, 0.1% IGEPAL, 1% SuperaseIn RNase Inhibitor (20 U/μL, Ambion), 1% BSA (20 mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 20ug of each sample were resolved by SDS-PAGE and Western blotted with following antibodies in TBST (Tris-buffered saline, 0.1% Tween 20): mouse anti-Flag at 1:1000 (Invitrogen) and rabbit anti-Vinculin at 1:2000 (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... 4 μL (1 μg) of sample was first trapped on an Acclaim PepMap 100 20 mm × 0.075 mm trapping column (ThermoFisher Cat# 164,535 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in TBS plus 0.1% Tween 20 at the following dilutions: AT8 (Thermo Fisher, 1:1000) and HT7 (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in wash buffer (PBS + 0.2% Tween 20) and incubated with DAPI staining solution (1 μg/ml, Invitrogen) for 10 min before mounted in Vectashield mounting medium (Vector Laboratories) ...
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bioRxiv - Biophysics 2022Quote: ... Laurell Technologies) with 20 μl of a 1:250 dilution of multifluorescent beads (TetraSpeck™ beads, ThermoFisher, 0.1 μm) allowing post-processing of the stage drift.
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bioRxiv - Cancer Biology 2024Quote: 2.5 × 105 cells were collected and washed with 1 ml of wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine (ThermoFisher, #13274)) before being resuspended in 1ml of wash buffer ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM EDTA), 20% SDS, and phenol:chloroform:isoamyl alcohol (25:24:1, pH 7.9) (Cat. No. 15593-049, Invitrogen, Waltham, MA) and bead beating with 0.1-mm diameter zirconia-silicate beads (Cat ...
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bioRxiv - Genomics 2024Quote: ... These selected colonies were grown in King’s B medium consisting of 20 g l-1 Bacto proteose peptone No.3 (Gibco), 1.5 g l-1 Potassium phosphate dibasic trihydrate (P5504 ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed three times for 10 min in DPBS + 0.05% Tween-20 (DPBS-T) and incubated with 1:1500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen) diluted in blocking solution for 1 hr ...
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bioRxiv - Biophysics 2023Quote: ... sonicated plasma membranes were fixed with 4% PFA before incubation with primary (GFP Thermo Fisher A11122 Rabbit 1/20) and secondary antibodies coupled to gold beads (rabbit-gold 815.011 AURION goat 1/25) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA libraries in the flowthrough were precipitated at −20°C for 18 hours in ethanol with 0.3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... in the presence of 20 nl ERCC spike-in per well (Ambion™ 4456740, 1:5000 diluted in H2O).
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was removed leaving ∼50 µL of buffer above the pellets and 500 µL of blocking buffer was added to resuspend before filtering through a 20 µm filter into FACS tubes and adding 1 µL of DAPI (0.1 mg/mL, D3571, Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... 1 μL of the reaction was diluted directly in 20 μL of formamide-containing denaturing RNA loading buffer (ThermoFisher) and incubated at 65°C for 10 min prior to its loading on a denaturing formaldehyde agarose gel (Fig ...
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bioRxiv - Biophysics 2023Quote: ... The flow cell is washed with 2 × 200 µL wash buffer (PBS supplemented with 0.02% v/v Tween-20 and 0.3% w/v BSA) before introducing 1-μm MyOne streptavidin-coated magnetic beads (Invitrogen). After 10 minutes incubation with magnetic beads ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were dried and incubated with 20 ng μL-1 trypsin protease (Pierce MS Grade, Thermo Fisher Scientific) solution in 100 mM NH4HCO3 pH 8.0 and 1 mM CaCl2 overnight at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The tissue sections were then rinsed in 0.1% PBS Tween-20 and stained with DAPI (1:10,000, Invitrogen, #D1306) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the tissue sections were incubated with a streptavidine FluorTM 660 conjugate (#50-4317-80, Thermo Fisher Scientific, 1:20) for 30 min at RT ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Actin filaments were stained using 1/20 dilution of a 200U stock solution containing Alexa Flour 647-phalloidin (Invitrogen) for two hours and washed with PBS + 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μL of washing buffer was added (Tris-HCL 10mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0,04 U/μL ...
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed again in PBS-T (0.05% Tween-20 in PBS) and PBS before incubation with 1:20,000 dilution of HRP-conjugated Streptavidin (Thermo scientific) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of total RNA was reverse transcribed in a 20 µl reaction using Taqman RT Reagents (ThermoFisher Scientific) with hexamers at 48°C for quantification of viral RNA copies or SuperScript IV Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with DPBS + Tween-20 and incubated with secondary antibodies for Pax7 (ThermoFisher #A-11008, 1:500) for 1 h at room temperature ...
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bioRxiv - Systems Biology 2024Quote: cDNA was produced in 20 µl reactions (1 per 10 µDNAse reaction) using Maxima Reverse transcriptase (#EP0743; ThermoFisher Scientific) and a gene specific (targeting the GFP reporter ...
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bioRxiv - Neuroscience 2024Quote: ... frozen coronal spinal cord sections (20 µm thick) were stained with FluoroMyelin green fluorescent myelin stain (1:1000, Invitrogen) and mounted with DAPI Fluoromount-G (Southern Biotech) ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA mixture was deacylated (37°C for 45 minutes in 20 mM Tris-HCl pH 9.0, RNaseOUT (1 µg/µL, Invitrogen) and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB) ...