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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 1 pulse) with 60 pmoles sgRNA (Synthego) and 20 pmoles SpCas9 nuclease (Synthego) using the Neon Transfection System (ThermoFisher Scientific) and the 10 μl tip per the manufacturer’s instructions.) ...
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bioRxiv - Immunology 2022Quote: ... monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco], 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], and 10 μg/ml gentamicin [Gibco]). The parental Prugniaud strain (PruΔHPT ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM D-Glucose, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 140 mM KCl and 20 mM HEPES, Invitrogen) for longer imaging sessions ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... the beads were washed four times by 1 ml IP buffer and resuspended in 20 µl 1x LDS sample buffer (Invitrogen) containing 2.5% β-mercaptoethanol and then boiled at 95 °C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were stained with DAPI (1 µg/ml in PBS) for 20 min at RT and Alexa Fluor647 NHS ester (A20006, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody diluted in PBS with 0.2% Tween- 20 and 1% Bovine Serum Albumin (BSA; #BP9700100; Fisher Scientific, Hampton, NH) was added to the apical chamber and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were recovered as outlined before,20 counted and diluted to 1×107/ml using pre-warmed complete medium (RPMI1640+Glutamax (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was removed after being centrifuged for 30 min at 20,000 × G and 1 mL of cold (4 °C) 80/20 methanol/water (Optima, LC/MS Grade, Fisher Scientific) was added to the remaining contents ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then delipidated by adding 1 mL of cold (−20 °C) isopropanol (Optima, LC/MS Grade, Fisher Scientific) and left overnight (12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... equilibrated with two resin-beds of equilibration buffer was added 1:20 lysate and end-over-end shaker (Thermo Fisher) incubated at 4C overnight ...
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bioRxiv - Cell Biology 2020Quote: DNA was stained using NucBlue Live (Hoechst 33342) according to manufacturer’s instructions (20 minutes, 1 drop/ml, RT, Thermo Fisher) or using Hoechst 33342 within mounting dye (VECTACHSIELD ...
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bioRxiv - Biochemistry 2021Quote: ... and 300 mM NaCl] was incubated with 20-fold excess (1 mM final concentration) of tetramethylrhodamine-5-maleimide (TMR) (Invitrogen) at room temperature in the dark for 2–4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mL per 50 mL of 50X B27 supplement and EGF 20 ng/ml (all from Life Technologies, Carlsbad, CA). After 5-7 days ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed once more with PBS + 0.1% Tween-20 prior to incubation in 1 μM DAPI (Life Technologies) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Newly synthesized cDNA was diluted 1:20 (vol:vol) in pure H2O and analyzed by qPCR using PowerUp SYBR Green Master Mix (Cat# A25742, ThermoFisher Scientific) on a CFX96 Real-Time PCR Detection System (Cat# 1855195 ...
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bioRxiv - Genomics 2020Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (Fisher Scientific 15437270). After electroporation ...
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bioRxiv - Microbiology 2020Quote: Cells were fixed with 2% PFA at room temperature for 20 min before incubating in 1:10,000 Hoescht 33342 (Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... 130-090-51) was from Miltenyi. FOXP3 (PCH1.1) (dilution: 1/20; Cat. Number: 53-4776-42) was from Thermo Fisher eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Plate was washed for three time with DPBS + 0.05 % Tween 20 (Scharlau, Spain) and then blocked with PBS + 1 % BSA (ThermoFisher, USA) + 0.050 % Tween-20 for 2 hours at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Plate was washed for 3 times with DPBS + 0.05 % Tween 20 (Scharlau, Spain) and then blocked with PBS + 1 % BSA (ThermoFisher, USA) + 0.050 % Tween 20 for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM l-glutamine, 1 mM sodium pyruvate, 30% L929-conditioned medium, 20% heat-inactivated fetal bovine serum [FBS; Invitrogen] ...
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bioRxiv - Neuroscience 2022Quote: ... or isolated human synaptoneurosomes from AD brains (Tai et al., 2014; Tzioras et al., 2019) tagged with pHrodo Red succinimidyl ester (1:20, ThermoFisher) by media change ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Cell Biology 2022Quote: ... analysis was performed in technical triplicates using 1:20 diluted cDNAs and 0.1 µM forward and reverse primers together with Power SYBR Green (Life Technologies) in a QuantStudio 7 Flex (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: Embryos/tadpoles were either injected intra-abdominally (at stage < 41) or bathed (at later stages) with/in a 1 mM 5-ethynyl-20-deoxyuridine solution (EdU, Invitrogen). They were then fixed after the desired time-period in 4% paraformaldehyde ...
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bioRxiv - Immunology 2022Quote: ... in tumor digestion medium (RPMI-1640 containing 10 mM HEPES, 1% penicillin/streptomycin and 20 μg/mL gentamicin; Gibco/Invitrogen) in a humidified incubator at 37°C with 5% CO2 with gentle rotation for 2-3h ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then blocked with 5% FBS in PBS for 20 min before stained with Alexa Flour 594 phalloidin (1:100, Invitrogen) for 1 h and 10 μg/ml DAPI (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample 1/20 of digests) was loaded onto a fused silica trap column (Acclaim PepMap 100, 75 μm × 2 cm, ThermoFisher). After washing for 5 min at 5 μl/min with 0.1% TFA ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with PBST for 40 minutes (20 mins x2) followed by incubation with a secondary antibody solution (1:200 Alexa 555 anti-rabbit [Invitrogen] ...
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bioRxiv - Neuroscience 2022Quote: ... Worms were immobilized with 20 mM sodium azide and mounted in a 1% low-melting-point agarose gel (UltraPure, Invitrogen). The TagBFP ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated LuECs were cultured with EC growth media (DMEM; Corning; 10-013-CV, 20% FBS, 1× Penicillin-Streptomycin-Glutamine; Gibco, USA ...
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bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated using 2.5% FBS neurobasal media (1X B27, 20 mM KCl, 1% Pen-Strep, 2 mM Glutamax (Thermo Fisher) 50 ng/mL Brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Microbiology 2024Quote: ... The slides were incubated for 1h at RT with primary antibody (1:20) Mouse Monoclonal Ab anti-AID (ZA001, Invitrogen), in PBS-0.1% BSA-0.4% Tween20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Bones were washed with BMDM media (20% FBS, 1% sodium pyruvate, 0.1% β-mercaptoethanol, 10% conditioned supernatant from 3T3 fibroblasts, in Gibco DMEM containing glucose and 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Developmental Biology 2024Quote: ... A mixture of hydrophilic and hydrophobic magnetic beads was used to clean-up the proteins at a ratio of 20:1 beads:proteins (Sera-Mag Speed beads, Fisher Scientific). After addition of ACN to a final concentration of 50% ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an overnight incubation at 4 °C with 20 μg ml-1 laminin (Thermo Fisher Scientific, 23-017-015). After being thoroughly washed with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 10∼20 ml of high-glucose DMEM (gibco) supplemented with 10% fetal bovine serum (FBS) (gibco) and 1% penicillin-streptomycin (ThermoFisher) were added ...
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bioRxiv - Neuroscience 2023Quote: ... and the secondary staining incubation lasted for 20 h and included streptavidin-conjugated Alexa Fluor 635 (1:1000, Molecular Probes) to label biocytin ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... tissue sections were digested during 1 hour at 37°C and at 300rpm (20 mg/mL proteinase K (Invitrogen 4333793) at 7.5 mg/mL in PKD buffer (Quiagen 1034963)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cas9 expression was induced by 200 ng/mL doxycycline followed by transfection with 20 nM equimolar crRNA:tracrRNA duplexes with 1:1000 RNAiMax (Life Technologies). Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then washed and incubated with 1 μg/ml DAPI/20% FCS/0.3% TritonX-100/PBS and either anti-rabbit Alexa Fluor 488 (Invitrogen, A11070) and anti-mouse Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei were stained with Hoechst (1:20) for 10 min in the dark and the coverslips were mounted in Immu-mount (ThermoFisher).
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 4-5 days at a ratio 1:15-20 using 0.5 mM EDTA (KD Medical, RGE-3130) or TrypLE Select (Gibco, 12563029) to dissociate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labelled with phycoerythrin (PE)-conjugated anti-human-HLA-ABC (clone W6/32, Invitrogen 12-9983-42; 1:20), biotin-conjugated anti-mouse-H-2Kb (clone AF6-88.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were washed three times with PBS + 0.05 % Tween 20 and stained with anti-rat AF564 (1:100, Invitrogen, A11007) and SYTOX™ Green (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Tissue culture was performed at 37°C in a 5 % CO2 humidified incubator using 1 ml of DMEM media supplemented with 20 % FBS (GIBCO), 100 U/ml penicillin (Invitrogen ...