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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The digested peptides were then desalted using Pierce C18 Spin Tips & Columns system according to the manufacturer’s instructions (ThermoFisher Scientific). Three independent biological replicates for each condition were analyzed by mass spectrometry.
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bioRxiv - Neuroscience 2024Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: Enriched crosslinked peptides were injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific™) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on a EASYspray C18 column (50 cm x 75 um, Thermo Fisher Scientific, San Jose, CA) over a 65-minute gradient at a flow rate of 250 nL/min ...
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bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 75 µm ID x 25 cm C18 Acclaim PepMap® RSLC column (Thermo Scientific, USA) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
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bioRxiv - Biochemistry 2024Quote: The tryptic peptides were injected onto a pre-column (PepMap C18, 5 mm × 300 μm × 5 μm, Thermo Fisher Scientific) and separated ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
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bioRxiv - Biochemistry 2024Quote: ... and the resulting peptides were analysed by LC-MSMS on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) using CID+ETD activation ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific) per channel ...
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bioRxiv - Molecular Biology 2024Quote: The resulting peptide mixtures were analyzed in data-dependent acquisition mode with an Orbitrap Exploris high-resolution mass spectrometer (MS) (ThermoFisher) coupled to an UltiMate 3000 LC system (Dionex-LC Packings) ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were first loaded onto an Acclaim PepMap C18 nano-trap column (100Å, 3µm, 75µm x 2cm, Thermo Scientific 164946) in mobile phase A ...
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bioRxiv - Physiology 2024Quote: ... and 10 μg of peptide per sample was labeled with TMTpro18 plex reagents (Cat. # A52045; Thermo Fisher Scientific, Darmstadt, Germany). TMT-labeled samples were adjusted to equal amounts and pooled ...
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bioRxiv - Systems Biology 2024Quote: ... 4 µg of peptides were analyzed in a QExactive mass spectrometer coupled to a nano EasyLC 1000 (Thermo Fisher Scientific). Solvent composition at the two channels was 0.1% (v/v ...
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bioRxiv - Plant Biology 2019Quote: ... Two μL (~1μg) peptides were analyzed by LC-MS with a Dionex RSLCnano HPLC coupled to a LTQ-Orbitrap Velos Pro (Thermo Scientific) mass spectrometer using a 2 h gradient ...
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bioRxiv - Biophysics 2021Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were separated on an EASY-Spray™ Acclaim PepMap C18 column (50 cm × 75 μm inner diameter, Thermo Fisher Scientific) using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptides were analyzed on a LC-MS/MS platform consisting of an Easy-nLC 1200 UHPLC system (Thermo Fisher Scientific) interfaced with an QExactive HF orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the extracted peptides were completely dried to completion and thereafter desalted using the SPE Pierce C18 Spin Columns (Thermo Fisher Scientific). These columns were activated by 2 × 200 μL of 50% acetonitrile (CAN ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described (36).
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bioRxiv - Molecular Biology 2022Quote: ... Target peptides for each of these proteins were purchased in stable isotope labelled synthetic peptides (SIS) form in crude quality from Thermo Scientific. The C-terminal lysine or arginine in the SIS peptides were replaced by isotope labelled lysine (13C6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
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bioRxiv - Cell Biology 2022Quote: Fractions of crosslinked peptides were analyzed by liquid chromatography coupled to tandem mass spectrometry using an LTQ Orbitrap Elite (Thermo Scientific) instrument 65 ...
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bioRxiv - Biochemistry 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Immunology 2019Quote: ... and resulting peptides were fractionated and sequenced by nanoflow LC-electrospray MS/MS on an Orbitrap Velos Pro hybrid mass spectrometer (Thermo Scientific), in the UT Austin mass spectrometry core facility as described previously (26) ...
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bioRxiv - Molecular Biology 2020Quote: ... High pH reversed fractionation was achieved by attaching a XBridge Peptide BEH C18 column (Cat. 186003570; Waters Corp.) to an Accela liquid chromatography system (Thermo Scientific) using the HPLC application ...
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bioRxiv - Molecular Biology 2020Quote: ... The column was enclosed into a column oven (Sonation) and the peptides were sprayed into a Q Exactive Plus mass spectrometer (Thermo Fisher). The mass spectrometer was operated in a data-dependent acquisition mode using a top10 method ...
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bioRxiv - Immunology 2022Quote: The encapsulation efficiency of the peptide into the NLC formulation was determined via the CBQCA protein quantitation kit (Thermo Fisher Scientific). Three separate NLC peptide formulations were created using the optimized formula previously determined in the DOE ...
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bioRxiv - Genomics 2020Quote: ... Flag peptide and small size of non-specific proteins were removed by 20K Slide-A-Lyzer dialysis cassette (ThermoFisher Scientific, 66003) with 1L binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to Sequelon-AA membrane (Applied Biosystems) as described previously [40].
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bioRxiv - Immunology 2019Quote: ... prior to injection of 2 µg of purified peptides into an Ultimate3000 nano-LC system coupled to a Q Exactive mass spectrometer (ThermoFisher Scientific) for mass spectrometry ...
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bioRxiv - Genetics 2020Quote: ... Peptide mixtures were analysed by nLC-ESI-MS/MS on a LTQ-Orbitrap XL coupled to an Easy nLC (Thermo Scientific). The sample preparation and the nLC-ESI-MS/MS analysis were performed as previously described (Rappsilber et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by MS measurement of peptides and their fragmentation spectra (LC-MS/MS) with a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2019Quote: The peptide samples resulting from the above digestion were separated by an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, USA). First ...
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bioRxiv - Plant Biology 2019Quote: The separated peptides were analyzed on-line by using a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operated in standard data-dependent acquisition mode controlled by Xcalibur version 4.0.27.19 ...
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bioRxiv - Synthetic Biology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1575 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
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bioRxiv - Cell Biology 2019Quote: ... in accordance with the manufacturer’s instructions and then fractionated using a Pierce high-pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Eight fractions were collected ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Peptides were separated on a column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, Thermo Fisher Scientific). The column had a 100 μm inner diameter microcapillary ...
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bioRxiv - Molecular Biology 2019Quote: Peptide fractions from the small-scale high pH reversed phase separation were analysed on an Orbitrap Tribrid Fusion (Thermo Fisher Scientific) with the same MS parameters as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Cell Biology 2019Quote: ... Extracted peptides were desalted and used for LC-MS/MS data acquisition on an Orbitrap Luminos mass spectrometer (Thermo Fisher Scientific) coupled with a 3000 Ultimate high-pressure liquid chromatography instrument (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 ml/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluted peptides were subjected to electrospray ionization coupled to an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo-Fisher Scientific) to produce tandem mass spectra whose b-and y-ion series patterns were compared to theoretical digests of the proteome in Sequest (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the peptides were separated with high-performance liquid chromatography (HPLC, Ultimate 3000 Nano LC System, Thermo Scientific, Rockwell, IL, USA) in a 15-cm long C18 column (Acclaim® PepMap™ RSLC inner diameter of 75 μm ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...