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Citations for Thermo Fisher :
3151 - 3200 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections were prepared from whole ovaries at 6 µm thickness and placed on charged glass slides (Fisher Scientific). Ovary sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T cells were seeded onto 6-well culture plates and transiently transfected with different PTH2R constructs using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h ...
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bioRxiv - Developmental Biology 2020Quote: Mouse small intestinal organoids derived from the duodenum of C57BL/6 mice were cultured in ENR medium: Advanced DMEM/F12 (Invitrogen) with 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were washed 3 × 5 minutes in PBS and finally mounted onto glass slides using Prolong Gold mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher). Images were acquired via confocal microscopy using a Zeiss LSM-880 confocal microscope (Plan-Apochromat 40x/1.3 Oil DIC UV-IR M27objective ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Microbiology 2021Quote: ... After 7 days of differentiation the BMDMs were seeded at a density of 0.8×106 cells/well in 6-well plates in MGM 10/10 media containing Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco®) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific) and allowed to set overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated onto 6-well plates and transfected the following day (70-80% confluence) using Lipofectamine 2000 (LF2000) (Invitrogen) for 4 h in DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2019Quote: ... in the iPSC media or in a feeder-free condition in StemFit AK02N (AJINOMOTO) in 6-cm dishes coated with 0.5 μg/cm2 vitronectin (Life Technologies). The cells were expanded using glass capillaries manually ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Molecular Biology 2021Quote: ... or stress granules (G3BP1 antibody, Supplementary File 6) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 400 ng of each RNA was used following supplier’s recommendations and carried out on a Quant 6 RT PCR (Applied Biosystems). Gene expression data was analyzed by excel and normalized to the mock transfected values ...
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bioRxiv - Immunology 2021Quote: ... Real time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). Raw Ct values were first normalized using the inter-plate calibrator UniSp3 ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... Protein samples either from ESCs or liver extracts were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5 % nonfat milk in PBS containing 0.1% Tween 20 (PBST) ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested 6 days following transfection and passed through a 0.45 μm filter prior to purification using CaptureSelect™ CH1-XL (ThermoFisher) affinity chromatography.
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bioRxiv - Immunology 2021Quote: The RBD was conjugated to CuMVTT using the cross-linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, MA) at 10-molar excess for 60 minutes at 23°C ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Cell Biology 2021Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... then incubated for 2h at room temperature with Alexa Fluor secondary antibodies (S2 Table) and nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Images were captured on a BX51 fluorescence microscope (Olympus ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression analysis was then performed by Real Time quantitative PCR (qPCR) using a QuantStudio 6 Flex thermocycler (Thermo Fisher). We used the following Taqman primers from Thermo Fisher (Supplementary Table 3) ...
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bioRxiv - Immunology 2021Quote: ... 293T cells were transduced as outlined above while being seeded at 20% confluency on either 6-well or 96-well plates (ThermoFisher). For 6-well plates ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Neuroscience 2022Quote: ... or 300K cells per 6-well tissue culture plates were seeded in Microglia Differentiation medium (Advanced DMEM-F12 (Gibco, #12634010) + 1x N2 supplement (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Hoechst 43222 (H1399) and ProLong Gold Antifade Mountant with 4′,6- diamidino-2-phenylindole (DAPI; P36931) were purchased from Invitrogen.
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064; 10 % horse serum, Gibco 26050-088 ...
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bioRxiv - Cell Biology 2022Quote: ... The plates were then washed with PBS 3×10min at RT on a shaking plate and cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064 ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was completed using the ThermoFisher Taqman Gene Expression Assays according to manufacturer’s protocol in the QuantStudio 6 Flex System (Applied Biosystems) mRNA targets included ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using the iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5% non-fat milk in TBS containing 0.1% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using SYBR Select Master Mix and QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with the following parameter ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed with 1xPBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306). Coverslips were then mounted in Vectashield (LSBio ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Cell Biology 2022Quote: PBMCs were thawed in a 37°C water bath and plated into a 6-well plate (353046, Falcon) with RPMI media (11875-093, Gibco), supplemented with 10% fetal bovine serum (900-108 ...