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Citations for Thermo Fisher :
3301 - 3350 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Peptides were eluted along an optimized 90 min gradient from 6 to 40% Mixture B (80% ACN/0.5% formic acid) with an EASY-nLC 1,200 system (Thermo Fisher Scientific). Spray voltage was set to 2.2 kV ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... of at least 6 were used for cDNA synthesis with the SuperScriptTM First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen). Real-time PCR was conducted using PowerUpTM SYBRTM Green Master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with a total of 1 µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2023Quote: ... then 36 cycles of 20s at 94°C and 60s at 54°C performed on an ABI9700 thermocycler followed by a final point read of the fluorescence on an ABI QuantStudio 6 real-time PCR system and using the QuantStudio software 1.3 (Applied Biosystems). For the genotyping of crude biological samples (whole blood or neutralized NaOH treatment solution of ear biopsies) ...
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bioRxiv - Genomics 2023Quote: ... then 36 cycles of 20 s at 94°C and 60 s at 54°C performed on an ABI9700 thermocycler followed by a final point read of the fluorescence on an ABI QuantStudio 6 real-time PCR system and using the QuantStudio software 1.3 (Applied Biosystems).
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bioRxiv - Biochemistry 2023Quote: Agar plate preparation: 7.5g agar (BD Difco™ Dehydrated Culture Media: Potato Dextrose Agar, Fisher Scientific, Cat# DF0013-17-6) was added to the 500 mL of liquid media ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded onto 0.1% gelatine-coated 6-well plates in DMEM supplemented with 10% foetal calf serum (Gibco, ThermoFischer, #26140079) and 1x Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Immunology 2023Quote: Purified CD4+ naïve T (Tresp) cells from CD45.1+ C57BL/6 mice were stained with CellTrace violet (CTV) (Thermo Fisher Scientific). Tresp cells (5 × 104 ...
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bioRxiv - Bioengineering 2023Quote: ... and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT–PCR system (Applied Biosystems). Relative quantities of viral and cellular RNA were calculated using the standard curve method ...
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bioRxiv - Bioengineering 2023Quote: PAAm gels prepared on coverslips were transferred to multiwell plates before covering with 0.2 mg/mL sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH) (Thermo Fisher) solution ...
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bioRxiv - Neuroscience 2023Quote: ... brains were washed trice with PBS for 5 min and free amines were anchored with 0.1mg/mL succinimidyl ester of 6-((Acryloyl)amino)hexanoic acid (Acryloyl-X, SE, Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded onto 6-well or 96-well plates for 24 hours and followed by siRNA (10 nM) transfection using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and 50 ng Myc-tagged GFP constructs were forward transfected into HFT cells seeded in a 6-well plate using Lipofectamine 3000 (Invitrogen). The cells were harvested 48 or 72 hours post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 January 2014) were thawed from cryovials and added to pre-warmed newborn calf serum (NCS, Gibco, Breda, The Netherlands), and centrifuged at 450g for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were plated 24 hours before transfection in a cell-culture treated 6-well plate (Fisher Scientific, 14-832-11). To transfect ...
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bioRxiv - Immunology 2023Quote: ... and a FAM-ZEN/Iowa Black FQ labeled probe sequence (Integrated DNA Technologies) on the QuantStudio 6 system (Applied Biosystems). An Ultramer DNA oligonucleotide spanning the amplicon (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... cranial neural crest induction was initiated (D0) by replacement of the stem cell maintenance media with neural crest induction media A (Essential 6 base medium (Gibco), 600nM CHIR99021 (Biogems) ...
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bioRxiv - Plant Biology 2023Quote: ... for 40 cycles (15 seconds at 95°C, 1 minute at 60°C) in a QuantStudio 6 Flex instrument (ThermoFisher). Percentage of input was calculated as 100* e(CtIN – log2(DF ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was centrifuged at 40,000 g and supernatant was loaded onto 6 ml of HisPure Ni-NTA resin (Thermo Scientific). The column was then washed with 7-column bed volumes (CV ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cultured to approximately 60% confluency on 24-or 6-well plate and transfected with siRNAs at final concentrations of 10-25nM using lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Neonatal HDFs were seeded in 6-well plates and transfected with 500ng of each plasmid per well using Lipofectamine 3000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... CRL-3216] and Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... and randomly distributed on 0.4µm Greiner Thincerts and placed in a 6-well plate containing 2ml DMEM/F12 (Thermo Fisher #31330038) + 10% FCS (Merck Millipore S0615 ...
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bioRxiv - Molecular Biology 2023Quote: The full length TF and corresponding IDR S2 cell lines were expanded to 6-well plates and prepared for imaging by resuspending and transferring to 27 mm glass bottom cell culture dishes (ThermoFisher). Cells were imaged on a Zeiss (Germany ...
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bioRxiv - Genomics 2023Quote: ... The IVT product was mixed with 6 μL ExoSAP-ITTM PCR Product Cleanup Reagent (Thermo Fisher Scientific, Cat. # 78200.200.UL) and incubated at 37°C for 15 mins ...
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were seeded in 6-well plates the day before transfection with the WSN reverse genetics plasmids in Opti-MEM (Gibco) using FuGene®6 (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Vero cells infected with unlabeled HSV-1 or OK15 at 10PFU/cell and were harvested from 6-well plates at the various timepoints and conditions indicated above using 0.3mL Trizol reagent (Life Technologies), and immediately stored at -20⁰C ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... a working solution of 0.83 mg/mL solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, 22589; Thermo Fisher) in PBS was prepared from a stock solution of 83 mg/mL sulfo-SANPAH in DMSO (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5×105 Tet-mCherry-CENP-A HeLa-TRex cells were transfected with 1ug of either CENP-C-LAP or empty vector plasmid in 6-well plates with Lipofectamine 3000 (Invitrogen) according to manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: Zebrafish larvae (6 day-post-fertilization) were incubated in embryo medium with 1% DMSO and 5 mM EdU (Invitrogen C10340) for 8 hours at 29°C after the 16 hour Vc infection ...
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...